2021
DOI: 10.21203/rs.3.rs-1153639/v1
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Pathogenesis of Aquatic Bird Bornavirus in Muscovy Ducks

Abstract: Aquatic bird bornavirus (ABBV), a type of avian bornavirus, has been associated with inflammation of the central and peripheral nervous systems and neurological disease in wild waterfowl in North America and Europe. The potential of ABBV to infect and cause lesions in commercial waterfowl species is unknown. The aim of this study was to determine the ability of ABBV to infect and cause disease in day-old Muscovy ducks (n = 174), selected as a representative domestic waterfowl. Ducklings became infected with AB… Show more

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Cited by 1 publication
(21 citation statements)
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“…All experimental procedures involving animals were approved by the University of Guelph Animal Care and Use Committee (Animal Utilization Protocol 3978), and conducted in accordance with the relevant regulations. The experimental outline is similar to what is reported in detail elsewhere (Iverson et al, 2021). A brief description follows.…”
Section: Experimental Designmentioning
confidence: 85%
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“…All experimental procedures involving animals were approved by the University of Guelph Animal Care and Use Committee (Animal Utilization Protocol 3978), and conducted in accordance with the relevant regulations. The experimental outline is similar to what is reported in detail elsewhere (Iverson et al, 2021). A brief description follows.…”
Section: Experimental Designmentioning
confidence: 85%
“…A suspension of cell-free virus was produced from immortalized duck embryo broblasts (American Type Culture Collection CCL-141) that were persistently infected with ABBV-1, as previously described (Iverson et al, 2021). Brie y, con uent 100-150 mm dishes of persistently infected CCL-141 cells were lysed with deionized water (osmotic shock) and one cycle of freeze/thaw in a ultra-cold freezer (-80 °C).…”
Section: Virus Inoculummentioning
confidence: 99%
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