Pathogen-responsive wheat PR4 genes are induced by activators of systemic acquired resistance and wounding

Bertini, Laura; Leonardi, Luca; Caporale, Carlo; Tucci, Marina; Cascone, Nunzia; Berardino, Iris Di; Buonocore, Vincenzo; Caruso, Carla

doi:10.1016/s0168-9452(03)00112-2

Cited by 67 publications

(52 citation statements)

References 44 publications

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“…TaPR4 genes were induced by treatment with the SA analog benzothiadiazole (BTH) and MeJA, indicating that TaWRKY78 is involved in both SA-and JA-dependent defense response pathways. 26 In Arabidopsis, WRKYs are most commonly associated with SA signaling, while wheat WRKY TFs examined in the present study have also been strongly influenced by the MeJA treatment (Fig. 2).…”

Section: Transcription Factorsmentioning

confidence: 55%

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Development of marker genes for jasmonic acid signaling in shoots and roots of wheat

Liu

Carvalhais

Kazan

2016

Plant Signaling & Behavior

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The jasmonic acid (JA) signaling pathway plays key roles in a diverse array of plant development, reproduction, and responses to biotic and abiotic stresses. Most of our understanding of the JA signaling pathway derives from the dicot model plant Arabidopsis thaliana, while corresponding knowledge in wheat is somewhat limited. In this study, the expression of 41 genes implicated in the JA signaling pathway has been assessed on 10 day-old bread wheat seedlings, 24 h, 48 h, and 72 h after methyljasmonate (MeJA) treatment using quantitative real-time PCR. The examined genes have been previously reported to be involved in JA biosynthesis and catabolism, JA perception and signaling, and pathogen defense in wheat shoots and roots. This study provides evidence to suggest that the effect of MeJA treatment is more prominent in shoots than roots of wheat seedlings, and substantial regulation of the JA pathway-dependent defense genes occurs at 72 h after MeJA treatment. Results show that the expression of 22 genes was significantly affected by MeJA treatment in wheat shoots. However, only PR1.1 and PR3 were significantly differentially expressed in wheat roots, both at 24 h post-MeJA treatment, with other genes showing large variation in their gene expression in roots. While providing marker genes on JA signaling in wheat, future work may focus on elucidating the regulatory function of JA-modulated transcription factors, some of which have well-studied potential orthologs in Arabidopsis.

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Section: Transcription Factorsmentioning

confidence: 55%

“…The activation of PR4a genes has been reported to be involved in both JA and SA-dependent defense response pathways. 26 Besides protecting wheat against fungal pathogens, wheatwin genes were developmentally regulated in the grain and may play a role in response to high temperatures.…”

Section: Pathogenesis-related Genesmentioning

confidence: 99%

Development of marker genes for jasmonic acid signaling in shoots and roots of wheat

Liu

Carvalhais

Kazan

2016

Plant Signaling & Behavior

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“…Wheatwin1, a pathogenesis-related protein of class 4 (PR4) from wheat, has antifungal activity against F. cumlorum (Caporale et al 2004). Induction of genes encoding wheatwin1 has been observed in wheat in response to wounding, F. culmorum infection and after treatments with resistance inducers (Bertini et al 2003). Thus, wounding might trigger some of the same defence responses as the resistance inducer treatments.…”

Section: Discussionmentioning

confidence: 99%

“…In wheat, induction of defence responses has been registered after pathogen attack, after treatment with fungal cell wall fractions and after treatment with several resistance inducers (Caruso et al 1999;Bertini et al 2003;Kuwabara et al 2002;Oka and Cohen 2001;Görlach et al 1996). Enhanced expression of genes often associated with induced disease resistance might also play a role in resistance to Fusarium spp.…”

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confidence: 99%

The effect of potential resistance inducers on development of Microdochium majus and Fusarium culmorum in winter wheat

et al. 2010

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The effect of potential resistance inducing chemicals on disease development of Fusarium head blight was studied in winter wheat (Triticum aestivum L.). As a pre-screening test, the effect of different treatments on development of Microdochium majus (syn. Microdochium nivale var. majus) was studied in detached leaves. Based on these tests, DL-3-aminobutyric acid, Bion (benzo-(1,2,3) thiadiazole-7-carbothioic acid S-methyl ester), and a foliar fertilizer containing potassium phosphite were selected for further studies. Greenhouse-grown winter wheat was sprayed with aqueous solutions of the potential resistance inducers 7 days prior to Fusarium culmorum point inoculation of the heads. Disease development was registered as number of bleached spikelets per inoculated spike. Spraying plants with the foliar fertilizer reduced the disease severity of F. culmorum by up to 40%. A reduced disease development of M. majus was also observed in detached leaves pre-treated with the foliar fertilizer. When the foliar fertilizer was added to the growth medium, a reduced in vitro growth of M. majus and F. culmorum was observed, indicating that the effect on disease development is at least partly due to a fungistatic effect. No significant reduction in disease development was observed in wheat pre-treated with DL-3-aminobutyric acid or Bion, although these compounds tended to reduce disease development, especially when applied in combination with other potential resistance inducers. We conclude that spraying winter wheat with a solution containing potassium phosphite can reduce development of M. majus and F. culmorum.

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“…All these four genes were significantly upregulated when the transgenic plants were infected with the pathogen, indicating that the abundant expression of AtPR1 and AtPR4 may be activated by the combined effect of pathogen inoculation and MrWRKY30 regulation. It is reported that PR1 and PR5 are SA inducible genes (Ward et al 1991;Yang et al 1997;Bertini et al 2003), while PR4 is induced by SA/JA (Bertini et al 2003), and PDF1.2 is JA/ET inducible gene (Penninckx et al 1998). The expression of MrWRKY30 in response to SA, JA, and ET in Noble showed that this gene may participate in the defense signaling pathways activated by these phytohormones.…”

Section: Mrwrky30 Is Involved In Downy Mildew Resistancementioning

confidence: 98%

Isolation of a WRKY30 gene from Muscadinia rotundifolia (Michx) and validation of its function under biotic and abiotic stresses

Jiang

Zhang

et al. 2015

Protoplasma

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WRKY transcription factors (TFs) play important roles in many plant processes, including responses to biotic and abiotic stresses. In the present study, Muscadinia rotundifolia MrWRKY30 dramatically accumulated in grapevine leaves in response to inoculation of Plasmopara viticola, a pathogen causing grapevine downy mildew disease. Similar responses were also found on grapevines treated with exogenous SA/JA/ET. Ectopic expression of MrWRKY30 in Arabidopsis thaliana "COL0" enhanced its resistance to downy mildew pathogen Peronospora parasitica. Pathogenesis-related (PR) genes, including AtPR1, AtPR4, AtPR5, and AtPDF1.2, were significantly upregulated in transgenic A. thaliana after P. parasitica inoculation. In the mean time, two critical genes in SA and JA signaling pathways, AtEDS5 and AtJAR1, were abundantly expressed as well, indicating that MrWRKY30 may enhance disease resistance of A. thaliana through SA and JA defense system. The transgenic A. thaliana plants also enhanced tolerance to cold stress accompanied with upregulation of AtCBF1, AtCBF3, AtICE1, and AtCOR47. MrWRKY30 might protect A. thaliana from cold damage by activating the AtCBF-mediated signaling pathway to induce the downstream AtCOR47 gene. Interestingly, the transgenic seedlings had a negative effect on salt tolerance. Reverse transcription PCR (RT-PCR) analysis revealed that antioxidant enzyme genes AtAPX (ascorbate peroxidase), AtCAT (catalase), and AtGST (glutathione-S-transferase) were suppressed in transgenic plants, which may lead to reactive oxygen species (ROS)-mediated sensitivity to salt stress.

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Pathogen-responsive wheat PR4 genes are induced by activators of systemic acquired resistance and wounding

Cited by 67 publications

References 44 publications

Development of marker genes for jasmonic acid signaling in shoots and roots of wheat

Development of marker genes for jasmonic acid signaling in shoots and roots of wheat

The effect of potential resistance inducers on development of Microdochium majus and Fusarium culmorum in winter wheat

Isolation of a WRKY30 gene from Muscadinia rotundifolia (Michx) and validation of its function under biotic and abiotic stresses

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