Pathogen‐Mimicking Polymeric Nanoparticles based on Dopamine Polymerization as Vaccines Adjuvants Induce Robust Humoral and Cellular Immune Responses

Liu, Qi; Jia, Jilei; Yang, Tingyuan; Fan, Qingze; Wang, Lianyan; Ma, Guanghui

doi:10.1002/smll.201503662

Cited by 52 publications

(36 citation statements)

References 80 publications

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“…It has been observed that the size of some particles affects the immune response [38,39]. PH GFP particles of different sizes were evaluated without finding a difference in the response of immunoglubulin G.…”

Section: Discussionmentioning

confidence: 99%

A self-aggregating peptide: implications for the development of thermostable vaccine candidates

et al. 2020

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Background: The use of biomaterials has been expanded to improve the characteristics of vaccines. Recently we have identified that the peptide PH (1-110) from polyhedrin self-aggregates and incorporates foreign proteins to form particles. We have proposed that this peptide can be used as an antigen carrying system for vaccines. However, the immune response generated by the antigen fused to the peptide has not been fully characterized. In addition, the adjuvant effect and thermostability of the particles has not been evaluated.Results: In the present study we demonstrate the use of a system developed to generate nano and microparticles carrying as a fusion protein peptides or proteins of interest to be used as vaccines. These particles are purified easily by centrifugation. Immunization of animals with the particles in the absence of adjuvant result in a robust and long-lasting immune response. Proteins contained inside the particles are maintained for over 1 year at ambient temperature, preserving their immunological properties. Conclusion:The rapid and efficient production of the particles in addition to the robust immune response they generate position this system as an excellent method for the rapid response against emerging diseases. The thermostability conferred by the particle system facilitates the distribution of the vaccines in developing countries or areas with no electricity.

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Section: Discussionmentioning

confidence: 99%

A self-aggregating peptide: implications for the development of thermostable vaccine candidates

et al. 2020

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“…Unfortunately, adoptive DC therapy is limited by high cost, inadequate cell sources, and safety concerns [66, 72]. Biomaterial approaches to modulate DC behavior have the potential to circumvent several limitations associated with exogenous delivery [31-35, 73-75]. In the present study, we have improved upon a dual-sized microparticle platform that previously demonstrated efficacy in the prevention of type 1 diabetes in NOD mice, and apply the therapy in the treatment of EAE, a mouse model of MS.…”

Section: Discussionmentioning

confidence: 99%

An antigen-specific semi-therapeutic treatment with local delivery of tolerogenic factors through a dual-sized microparticle system blocks experimental autoimmune encephalomyelitis

et al. 2017

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Antigen-specific treatments are highly desirable for autoimmune diseases in contrast to treatments which induce systemic immunosuppression. A novel antigen-specific therapy has been developed which, when administered semi-therapeutically, is highly efficacious in the treatment of the mouse model for multiple sclerosis, namely experimental autoimmune encephalomyelitis (EAE). The treatment uses dual-sized, polymeric microparticles (dMPs) loaded with specific antigen and tolerizing factors for intra- and extra-cellular delivery, designed to recruit and modulate dendritic cells toward a tolerogenic phenotype without systemic release. This approach demonstrated robust efficacy and provided complete protection against disease. Therapeutic efficacy required encapsulation of the factors in controlled-release microparticles and was antigen-specific. Disease blocking was associated with a reduction of infiltrating CD4+ T cells, inflammatory cytokine-producing pathogenic CD4+ T cells, and activated macrophages and microglia in the central nervous system. Furthermore, CD4+ T cells isolated from dMP-treated mice were anergic in response to disease-specific, antigen-loaded splenocytes. Additionally, the frequency of CD86hiMHCIIhi dendritic cells in draining lymph nodes of EAE mice treated with Ag-specific dMPs was reduced. Our findings highlight the efficacy of microparticle-based drug delivery to mediate antigen-specific tolerance, and suggest that such a multi-factor combinatorial approach can act to block autoimmunity.

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“…Ma et al incorporated a hepatitis B surface antigen (HBsAg) and CpG adjuvant onto PLGA nanoparticles via conjugation with dopamine, allowing the particles to display both the viral antigen and the immune activator. The study showed that the pathogen-mimicking particle enhanced the recruitment of immune cells to the injected site, activated bone marrow derived dendritic cells, and induced strong humoral and cellular immune responses [164]. In another study conducted by Kuai et al, a disc-like synthetic high-density lipoprotein (sHDL) was applied to integrate both target antigens of CD8+ T cells and CpG-ODN for anticancer vaccination.…”

Section: Co-delivery Of Antigens and Adjuvants By Nanoparticle Vaccinesmentioning

confidence: 99%

“…Dopamine-incorporated polymers have also been employed to adsorb proteins for macrophage-targeted delivery [205]. The versatile technique can be applied to conjugate multiple cargoes for vaccine applications [164].…”

Section: Chemical Conjugationmentioning

confidence: 99%

Nanoparticle Vaccines Adopting Virus-like Features for Enhanced Immune Potentiation

et al. 2017

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Synthetic nanoparticles play an increasingly significant role in vaccine design and development as many nanoparticle vaccines show improved safety and efficacy over conventional formulations. These nanoformulations are structurally similar to viruses, which are nanoscale pathogenic organisms that have served as a key selective pressure driving the evolution of our immune system. As a result, mechanisms behind the benefits of nanoparticle vaccines can often find analogue to the interaction dynamics between the immune system and viruses. This review covers the advances in vaccine nanotechnology with a perspective on the advantages of virus mimicry towards immune potentiation. It provides an overview to the different types of nanomaterials utilized for nanoparticle vaccine development, including functionalization strategies that bestow nanoparticles with virus-like features. As understanding of human immunity and vaccine mechanisms continue to evolve, recognizing the fundamental semblance between synthetic nanoparticles and viruses may offer an explanation for the superiority of nanoparticle vaccines over conventional vaccines and may spur new design rationales for future vaccine research. These nanoformulations are poised to provide solutions towards pressing and emerging human diseases.

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Pathogen‐Mimicking Polymeric Nanoparticles based on Dopamine Polymerization as Vaccines Adjuvants Induce Robust Humoral and Cellular Immune Responses

Cited by 52 publications

References 80 publications

A self-aggregating peptide: implications for the development of thermostable vaccine candidates

A self-aggregating peptide: implications for the development of thermostable vaccine candidates

An antigen-specific semi-therapeutic treatment with local delivery of tolerogenic factors through a dual-sized microparticle system blocks experimental autoimmune encephalomyelitis

Nanoparticle Vaccines Adopting Virus-like Features for Enhanced Immune Potentiation

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