A self-aggregating peptide: implications for the development of thermostable vaccine candidates

Abstract

Background: The use of biomaterials has been expanded to improve the characteristics of vaccines. Recently we have identified that the peptide PH (1-110) from polyhedrin self-aggregates and incorporates foreign proteins to form particles. We have proposed that this peptide can be used as an antigen carrying system for vaccines. However, the immune response generated by the antigen fused to the peptide has not been fully characterized. In addition, the adjuvant effect and thermostability of the particles has not been evaluated.Results: In the present study we demonstrate the use of a system developed to generate nano and microparticles carrying as a fusion protein peptides or proteins of interest to be used as vaccines. These particles are purified easily by centrifugation. Immunization of animals with the particles in the absence of adjuvant result in a robust and long-lasting immune response. Proteins contained inside the particles are maintained for over 1 year at ambient temperature, preserving their immunological properties. Conclusion:The rapid and efficient production of the particles in addition to the robust immune response they generate position this system as an excellent method for the rapid response against emerging diseases. The thermostability conferred by the particle system facilitates the distribution of the vaccines in developing countries or areas with no electricity.

Candidate Effectors from Botryosphaeria dothidea Suppress Plant Immunity and Contribute to Virulence

“…For target gene deletion, polyethylene glycol (PEG)-mediated homologous-recombination gene deletion was performed and verified while using previously described strategies [ 10 ]. We generated a gene homologous recombination (GHR) plasmid containing a hygromycin-resistance gene ( Hph ) with flanking sequences of the target gene.…”

“…A cassette, including the 5′- and 3′-flanking sequences of the target gene, was amplified from the GHR plasmid and then transformed into the protoplasts of HTLW03 by the PEG-mediated transformation method, and the gene deletion transformants were screened, as described by Dong and Guo [ 10 ]. The gene deletion mutants were verified by PCR while using the primer pairs that are listed in Table S5 .…”

“…Although genomic data for B. dothidea are available [ 1 , 8 , 9 ], the pathogenesis of B. dothidea is still unknown, which is mainly due to the lack of gene disruption protocol for this pathogen. Our recently established gene deletion protocol [ 10 ] makes genetic manipulation of this coenocytic woody plant pathogen possible.…”

Plasmid Replicons for the Production of Pharmaceutical-Grade pDNA, Proteins and Antigens by Lactococcus lactis Cell Factories

“…The FnbpA mediates the invasivity of L. lactis in nonphagocytic host cells, improving its ability to deliver DNA. Zurita-Turk et al [ 72 ] successfully used this invasive strain to deliver interleukin-10 (IL-10) to IL-10 deficient mice, using pValac as the gene-carrying plasmid.…”

The Cutinase Bdo_10846 Play an Important Role in the Virulence of Botryosphaeria dothidea and in Inducing the Wart Symptom on Apple Plant

“…However, their roles in the infection process are unknown, mainly due to the lack of gene disruption protocol for this pathogen. Our recently established gene deletion protocol [ 39 ] makes genetic manipulation of B. dothidea possible. In this study, we investigate the mechanism of warts formation on apple shoots and explore the role of a cutinase gene in the interaction between B. dothidea and the apple ( Malus domestica ) plant.…”