Pathogen-host interaction mediated by vesicle-based secretion in schistosomes

Bischofsberger, Miriam; Winkelmann, Franziska; Rabes, Anne; Reisinger, Emil C.; Sombetzki, Martina

doi:10.1007/s00709-020-01515-y

Cited by 7 publications

(6 citation statements)

References 117 publications

(129 reference statements)

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“…A glutamine synthetase has been described having high expression in the S. japonicum 21-day schistosomulum and adult worms of that and has been suggested as a drug target [ 47 ]. None of these three proteins have been identified in extracellular vesicles studies in our work [ 48 ].…”

Section: Discussionmentioning

confidence: 93%

T Cell Peptides Derived from Invasive Stages of Schistosoma mansoni as Potential Schistosomiasis Vaccine

López‐Abán

Vicente

Kabbas-Piñango

et al. 2021

JCM

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Schistosomiasis is a parasitic disease that affects 143 million people in endemic countries. This work analyzed overexpressed sequences from the cercaria phase to the early schistosomulum phase using bioinformatics tools to predict host interaction and selected proteins for predicting T cell epitopes. The final peptides were chemically synthesized, and their toxicity was evaluated in vitro. Peptides were formulated in the Adjuvant Adaptation (ADAD) vaccination system and injected into BALB/c mice that were challenged with S. mansoni cercariae to assess protection and immunogenicity. A total of 39 highly expressed S.mansoni proteins were identified as being of potential interest. Three T cell peptides predicted to bind MHC mouse and human class II were synthesized and formulated for vaccination. SmGSP and SmIKE reduced the number of eggs trapped in the liver by more than 50% in challenged BALB/c mice. The liver of mice vaccinated with either SmGSP or SmTNP had a significantly reduced affected liver surface. Transcriptome-based T cell peptides elicit partial protection and could be candidates for a multiantigen vaccine.

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Section: Discussionmentioning

confidence: 93%

T Cell Peptides Derived from Invasive Stages of Schistosoma mansoni as Potential Schistosomiasis Vaccine

López‐Abán

Vicente

Kabbas-Piñango

et al. 2021

JCM

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“…The highest lncRNA expression level in liver samples was veri ed in Sm-lncRNA 43 and Sm-lncRNA 55. In addition to these two molecules, Sm-lncRNA 19,24,25,31,37,44,48,54, and 59 also presented high expression levels in mouse liver samples.…”

Section: S Mansoni Lncrna Expression In Mouse Liversmentioning

confidence: 99%

Profiles of LncRNAs expression in Schistosoma mansoni during intra- mammalian development

Rocha

Oliveira

Castro-Borges

et al. 2022

Preprint

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Background Schistosomes infect over 200 million people, resulting in chronic disease and hundreds of thousands of deaths. It is believed that the complexity of the differentiation and developmental programs observed among the different developmental stages and in the environments where the parasite lives are influenced by the regulation of gene expression. In this scenario, long noncoding RNAs (lncRNAs) would be key molecules. Recently, our research group identified a set of 170 new lncRNAs in Schistosoma mansoni, and 15 were experimentally analyzed. Continuing this investigation, we evaluated the expression of 47 of these lncRNAs in S. mansoni developmental stages, as well as in liver samples of a murine schistosomiasis model. Methods We experimentally analyze 47 S. mansoni lncRNAs, and the validated ones had their expression analyzed using RT-qPCR in samples of the definitive-host-related developmental stages of the worm: cercariae, schistosomula 3.5 h, adult worms, and eggs; as well as in liver samples of BALB/c mice infected (100 cercariae) and uninfected, collected 9 weeks post-infection. Putative target protein-coding genes expression and Gene Ontology were collected in WormBase Parasite. Results We demonstrate that 25 of the 47 lncRNAs analyzed were primarily expressed in adult worms. 20 of these also show to be differentially expressed among the developmental stages evaluated, demonstrating that these molecules probably play a role in stage-specific gene expression. We also detect the expression of 22 S. mansoni-specific lncRNAs host liver samples, suggesting the action in the host-pathogen relationship. Finally, we describe lncRNA–protein coding gene correlations that identify lncRNAs with prospective roles in gene regulation. Conclusions Our results show clear differential expression patterns of lncRNAs in host-dependent development stages of S. mansoni and ascribe potential functional roles in development based on predicted intracellular interactions as well as potential for being biomarkers.

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“…EVs are secreted by almost all types of cells, and contents of EVs include proteins, lipids, polysaccharides, and other molecules such as nucleic acids ( O'Brien et al., 2020 ). Schistosome EV microRNAs (miRNAs) cargo had been shown to modulate the host immune response ( Liu et al., 2019 ; Bischofsberger et al., 2020 ; Meningher et al., 2020 ). Moreover, the contents of the EVs released within an infected host can serve as promising biomarkers for the disease diagnosis and help in understanding the infection-induced host pathology ( Meningher et al., 2017 ; Cai et al., 2020 ).…”

Section: Introductionmentioning

confidence: 99%

Characterization of MicroRNA Cargo of Extracellular Vesicles Isolated From the Plasma of Schistosoma japonicum-Infected Mice

Giri

Liu

et al. 2022

Front. Cell. Infect. Microbiol.

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Schistosoma is a genus of parasitic trematodes that undergoes complex migration in final hosts, finally developing into adult worms, which are responsible for egg production and disease dissemination. Recent studies documented the importance of extracellular vesicles (EVs) in the regulation of host-parasite interactions. Herein, we investigated the microRNA (miRNA) profiles of EVs isolated from host plasma at different stages of Schistosoma japonicum infection (lung stage: 3 days post-infection (dpi), and liver stages: 14 and 21 dpi) to identify miRNA cargo potentially involved in the pathogenesis and immune regulation of schistosomiasis. Characterization of the isolated plasma EVs revealed their diameter to be approximately 100 nm, containing typical EV markers such as Hsp70 and Tsg101. Deep sequencing analysis indicated the presence of 811 known and 15 novel miRNAs with an increasing number of differential miRNAs from the lung stage (27 miRNAs) to the liver stages (58 and 96 miRNAs at 14 and 21 dpi, respectively) in the plasma EVs of infected mice compared to EVs isolated from the uninfected control. In total, 324 plasma EV miRNAs were shown to be co-detected among different stages of infection and the validation of selected miRNAs showed trends of abundance similar to deep sequencing analysis. For example, miR-1a-3p and miR-122-5p showed higher abundance, whereas miR-150-3p and miR-126a showed lower abundance in the plasma EVs of infected mice at 3, 14, and 21 dpi as compared to those of uninfected mice. In addition, bioinformatic analysis combined with PCR validation of the miRNA targets, particularly those associated with the immune system and parasitic infectious disease, indicated a significant increase in the expression of Gbp7and Ccr5 in contrast to the decreased expression of Fermt3, Akt1, and IL-12a. Our results suggested that the abundance of miRNA cargo of the host plasma EVs was related to the stages of Schistosoma japonicum infection. Further studies on the roles of these miRNAs may reveal the regulatory mechanism of the host-parasite interaction. Moreover, the differentially abundant miRNA cargo in host EVs associated with S. japonicum infection may also provide valuable clues for identifying novel biomarkers for schistosomiasis diagnosis.

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Pathogen-host interaction mediated by vesicle-based secretion in schistosomes

Cited by 7 publications

References 117 publications

T Cell Peptides Derived from Invasive Stages of Schistosoma mansoni as Potential Schistosomiasis Vaccine

T Cell Peptides Derived from Invasive Stages of Schistosoma mansoni as Potential Schistosomiasis Vaccine

Profiles of LncRNAs expression in Schistosoma mansoni during intra- mammalian development

Characterization of MicroRNA Cargo of Extracellular Vesicles Isolated From the Plasma of Schistosoma japonicum-Infected Mice

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