Schistosomiasis is a parasitic disease that affects 143 million people in endemic countries. This work analyzed overexpressed sequences from the cercaria phase to the early schistosomulum phase using bioinformatics tools to predict host interaction and selected proteins for predicting T cell epitopes. The final peptides were chemically synthesized, and their toxicity was evaluated in vitro. Peptides were formulated in the Adjuvant Adaptation (ADAD) vaccination system and injected into BALB/c mice that were challenged with S. mansoni cercariae to assess protection and immunogenicity. A total of 39 highly expressed S.mansoni proteins were identified as being of potential interest. Three T cell peptides predicted to bind MHC mouse and human class II were synthesized and formulated for vaccination. SmGSP and SmIKE reduced the number of eggs trapped in the liver by more than 50% in challenged BALB/c mice. The liver of mice vaccinated with either SmGSP or SmTNP had a significantly reduced affected liver surface. Transcriptome-based T cell peptides elicit partial protection and could be candidates for a multiantigen vaccine.
Hydroxylated monoterpenes (HMTPs) are differentially emitted by tomato plants efficiently resisting a bacterial infection. We have studied the defensive role of these volatiles in the tomato response to bacteria, whose main entrance are stomata apertures. Treatments with some HMTPs resulted in stomatal closure andPR1induction. Particularly, α-terpineol induced stomatal closure in a salicylic (SA) and abscisic acid-independent manner, and conferred resistance to bacteria. Interestingly, transgenic tomato plants overexpressing or silencing the monoterpene synthaseMTS1,which displayed alterations in the emission of HMTPs, exhibited changes in the stomatal aperture but not in plant resistance. Measures of both 2-C-methyl-D-erythritol-2,4-cyclopyrophosphate (MEcPP) and SA levels, revealed a competition for MEcPP by the methylerythritol phosphate (MEP) pathway and the SA biosynthesis activation, thus explaining the absence of phenotype in transgenic plants. These results were confirmed by chemical inhibition or activation of the MEP pathway. Besides, treatments with BTH, a SA functional analogue, conferred enhanced resistance in transgenic tomato plants overexpressingMTS1.Finally, plants overexpressingMTS1inducedPR1and stomata closure in neighbouring plants. Our results confirm the role of HMTPs in both intra and inter-plant immune signalling, and reveal a metabolic crosstalk between the MEP and SA pathways in tomato plants.Graphical AbstractMetabolic crosstalk between hydroxylated monoterpenes and salicylic acid in tomato defence response against Pseudomonas syringae pv tomato. Created with BioRender.com.
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