Pathobiology of cigarette smoke-induced invasive cancer of the renal pelvis and its prevention by vitamin C

Ganguly, Shinjini; Chandra, Ayan; Chatterjee, Indu B.

doi:10.1016/j.toxrep.2018.10.005

Cited by 11 publications

(12 citation statements)

References 41 publications

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“…Cells were lysed in RIPA lysis buffer (20 mM HEPES and 0.5% Triton X-100, pH 7.6) and separated by 10% SDS-PAGE [42]. Proteins were transferred to nitrocellulose membranes (GE Healthcare Life Science, Pittsburgh, PO, USA) and checked using an Immobilon Western Chemiluminescent HRP Substrate (EMD Millipore, Darmstadt, Germany) for analysis protein expression.…”

Section: Methodsmentioning

confidence: 99%

Axl Inhibitor R428 Enhances TRAIL-Mediated Apoptosis Through Downregulation of c-FLIP and Survivin Expression in Renal Carcinoma

Woo

Min

Seo

et al. 2019

IJMS

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R428, a selective small molecule Axl inhibitor, is known to have anti-cancer effects, such as inhibition of invasion and proliferation and induction of cell death in cancer cells. The Axl receptor tyrosine kinase is highly expressed in cancer cells and the level of Axl expression is associated with survival, metastasis, and drug resistance of many cancer cells. However, the effect of Axl inhibition on overcoming anti-cancer drugs resistance is unclear. Therefore, we investigated the capability of Axl inhibition as a therapeutic agent for the induction of TRAIL (tumor necrosis factor-related apoptosis-inducing ligand) sensitivity. In this study, R428 markedly sensitized cancer cells to TRAIL-induced apoptotic cell death, but not in normal human skin fibroblast (HSF) and human umbilical vein cells (EA.hy926). Moreover, knockdown of Axl by siRNA also increased TRAIL-induced apoptosis. R428 decreased c-FLIP proteins levels via induction of miR-708 expression and survivin protein levels at the post-translational level, and we found that knockdown of Axl also decreased both c-FLIP and survivin protein expression. Overexpression of c-FLIP and survivin markedly inhibited R428 plus TRAIL-induced apoptosis. Furthermore, R428 sensitized cancer cells to multiple anti-cancer drugs-mediated cell death. Our results provide that inhibition of Axl could improve sensitivity to TRAIL through downregulation of c-FLIP and survivin expression in renal carcinoma cells. Taken together, Axl may be a tempting target to overcome TRAIL resistance.

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Section: Methodsmentioning

confidence: 99%

Axl Inhibitor R428 Enhances TRAIL-Mediated Apoptosis Through Downregulation of c-FLIP and Survivin Expression in Renal Carcinoma

Woo

Min

Seo

et al. 2019

IJMS

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“…As it was reported most patients who rated CAT therapy as helpful, a little helpful and very helpful (30.3%, 23.23% and 20.2%, respectively) and only 22 of 198 patients (11.11%) rated it as not helpful at all. It should be recalled that some studies suggest the effectiveness of CAT in supporting conventional therapy [1,20,21].…”

Section: Discussionmentioning

confidence: 99%

The use of complementary and alternative therapy by advanced cancer patients receiving palliative care at home

et al. 2022

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Introduction:The purpose of this study was to evaluate the use of complementary and alternative therapies (CAT) by palliative care patients treated at home in Poland. Patients and methods: A total of 241 adult patients with advanced or metastatic cancer who were qualified for palliative care provided at home filled out the CAT screening tool. Data were analysed to assess CAT use association with several variables. Results: 82.16% of individuals who completed the survey declared using CAT at least once in the last 12 months. Self-help practices were the most used CAT category (74.47%), it was followed by herbal medicine and dietary supplements (62.66%) and visits to CAT providers (41.91%). CAT use was more prevalent among women, patients with basic education, and patients currently married and widowed. The most common reason pointed for using CAT was to improve well-being (35.4%). 50.5% of CAT users declared that they find used therapy helpful or very helpful. The study revealed an exceptionally high prevalence of spiritual practices (self-prayer, spiritual healing) in comparison to previous European studies conducted among the cancer patient population. Conclusions:The study indicated that usage of CAT among advanced cancer patients treated at home is significant, with a higher prevalence of spiritual practices than reported in previous studies among cancer patients in Europe.

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“…Cells were lysed in radioimmunoprecipitation assay (RIPA) lysis buffer (20 mM HEPES and 0.5% Triton X-100, pH 7.6) and separated by 10% SDS-PAGE [ 28 ]. Proteins were transferred to nitrocellulose membranes (GE Healthcare Life Science, Pittsburgh, PA, USA) and detected using an Immobilon Western Chemiluminescent HRP Substrate (EMD Millipore, Darmstadt, Germany) for analysis protein expression.…”

Section: Methodsmentioning

confidence: 99%

Inhibition of Drp1 Sensitizes Cancer Cells to Cisplatin-Induced Apoptosis through Transcriptional Inhibition of c-FLIP Expression

2020

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Mitochondrial fragmentation occurs during the apoptosis. Dynamin-related protein 1 (Drp1) acts as an important component in mitochondrial fission machinery and can regulate various biological processes including apoptosis, cell cycle, and proliferation. The present study demonstrates that dysfunction of mitochondrial dynamics plays a pivotal role in cisplatin-induced apoptosis. Inhibiting the mitochondrial fission with the specific inhibitor (Mdivi-1) did not affect apoptotic cell death in low concentrations (<10 μM). Interestingly, mdivi-1 enhanced cisplatin-induced apoptosis in cancer cells, but not in normal cells. Particularly in the presence of mdivi-1, several human cancer cell lines, including renal carcinoma cell line Caki-1, became vulnerable to cisplatin by demonstrating the traits of caspase 3-dependent apoptosis. Combined treatment induced downregulation of c-FLIP expression transcriptionally, and ectopic expression of c-FLIP attenuated combined treatment-induced apoptotic cell death with mdivi-1 plus cisplatin. Collectively, our data provide evidence that mdivi-1 might be a cisplatin sensitizer.

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Pathobiology of cigarette smoke-induced invasive cancer of the renal pelvis and its prevention by vitamin C

Abstract: Graphical abstract

Cited by 11 publications

References 41 publications

Axl Inhibitor R428 Enhances TRAIL-Mediated Apoptosis Through Downregulation of c-FLIP and Survivin Expression in Renal Carcinoma

Axl Inhibitor R428 Enhances TRAIL-Mediated Apoptosis Through Downregulation of c-FLIP and Survivin Expression in Renal Carcinoma

The use of complementary and alternative therapy by advanced cancer patients receiving palliative care at home

Inhibition of Drp1 Sensitizes Cancer Cells to Cisplatin-Induced Apoptosis through Transcriptional Inhibition of c-FLIP Expression

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