Passive transfer of immunity with serum in mice infected with Nematospiroides dubius: In vitro effect of immune serum on larval infectivity

Dobson, Colin; Cayzer, Catherine J.R.

doi:10.1016/0020-7519(82)90070-4

Cited by 18 publications

(5 citation statements)

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“…Most strains of mice acquire some capacity for resisting H. polygyrus following exposure to repeated or abbreviated infection protocols (Prowse, Mitchell, Ey & Jenkin, 1979;Behnke & Robinson, 1985) and the mechanisms involved have been studied in several laboratories in recent years (Dobson & Cayzer, 1982;Jacobson, Brooks & Cypess, 1982;Pritchard, Williams, Behnke & Lee, 1983;Pentilla, Ey, Lopez & Jenkin, 1985). However, immunological events during the chronic primary infection, particularly in the terminal stages, 6-10 months after initial exposure to larvae, have been relatively neglected.…”

Section: Introductionmentioning

confidence: 99%

Immunological relationships during primary infection with Heligmosomoides polygyrus (Nematospiroides dubius): the capacity of adult worms to survive following transplantation to recipient mice

et al. 1987

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Section: Introductionmentioning

confidence: 99%

Immunological relationships during primary infection with Heligmosomoides polygyrus (Nematospiroides dubius): the capacity of adult worms to survive following transplantation to recipient mice

et al. 1987

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“…In these circumstances it is not surprising that NMlgAgs had similar electrophoretic properties to some of the components bound by IMIg in affinity chromatography and that immune responses to them were amplified to the possible benefit of N. dubius. N. dubius larvae bathed in IMS survived better in mice passively immunized with IMS than larvae bathed in NMS, which further illuminates this effect (22).…”

Section: Discussionmentioning

confidence: 66%

Mice vaccinated against Nematospiroides dubius with antigens isolated by affinity chromatography from adult worms

Monroy

Dobson

1987

Immunol Cell Biol

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Summary.A crude soluble extract (AWH) obtained from homogenized adult Nematospiraides dubius worms was fractionated into normal (NMtgAg) and immune (IMlgAg) antigens by sequential passage through Sepharose 4B, and CNBr-activaled Sepharose 4B to which immunoglobulins from normal (NMIg) and N. d»ft(U5-infected mouse serum (IMIg) had been coupled. Sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGF) under reducing condilions showed that MNtgAgand IMtgAg contained several proteins with molecular weights (MW) between 20,000 and 200,0(X). IMtgAg contained 2 polypeptides, MW 24,000 and 55,(XX), which reacted strongly in western bloi analysis against IMig; the larger molecule related lo a cuticular component of the worm. Protective high MW AWH G-200 filtrates shared 5 polypeptides with IMlgAg (MW 85,000-200.000) together with several smaller molecules (MW 30,000-< 10,000). The MW 55,O(X) and 24.0(X) components concentrated in JMlgAg were restricted to non-protective low MW AWH gel fiUraies. BCM mice vaccinated with IMtgAg were protected againsi N. dubius. Worm numbers, size and feciindily were reduced to an extent commensurate with that obtained in mice vaccinated wiih AWH. Mice vaccinaled wiih NMIgAg showed partial resistance. The role of surface and dominant parasite immunogens in immunity is discussed.

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“…dubius . Moreover, Dobson and Cayzer (1982) found that N. dubius larvae bathed in immune mouse serum survived better in mice passively immunized with immune mouse serum than larvae bathed in normal mouse serum. Similarly, Ogilvie (1971) found that adult N. brasiliensis survived transplantation from rats to the intestines of mice which had been passively immunized with either rat or mouse antiserum.…”

Section: Discussionmentioning

confidence: 99%

SPECIFICITY OF PASSIVE SERUM PROTECTION AGAINST NIPPOSTRONGYLUS BRASILIENSIS AND NEMATOSPIROIDES DUBIUS IN MICE

Brindley

Dobson

1983

Aust J Exp Biol Med

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Summary Fewer and smaller worms were recovered from, and significantly fewer parasite eggs were voided by, mice injected with mouse antiserum raised against Nippostrongylus brasiliensis or Nematospiroides dubius after infection with the homologous nematode species compared with the control mice. The passive transfer of protective immunity was specific for the species which induced the antibody response in the donor mice. Passively immunized mice infected with the heterologous nematode species harboured the same number of worms, with the same epg output, as parasites in the control mice, but were stunted. Serological cross‐reactions were observed between the two donor antiserum pools and N. dubius and N. brasiliensis antigens.

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Passive transfer of immunity with serum in mice infected with Nematospiroides dubius: In vitro effect of immune serum on larval infectivity

Cited by 18 publications

References 19 publications

Immunological relationships during primary infection with Heligmosomoides polygyrus (Nematospiroides dubius): the capacity of adult worms to survive following transplantation to recipient mice

Immunological relationships during primary infection with Heligmosomoides polygyrus (Nematospiroides dubius): the capacity of adult worms to survive following transplantation to recipient mice

Mice vaccinated against Nematospiroides dubius with antigens isolated by affinity chromatography from adult worms

SPECIFICITY OF PASSIVE SERUM PROTECTION AGAINST NIPPOSTRONGYLUS BRASILIENSIS AND NEMATOSPIROIDES DUBIUS IN MICE

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