Immunological relationships during primary infection with <i>Heligmosomoides polygyrus</i> (<i>Nematospiroides dubius</i>): the capacity of adult worms to survive following transplantation to recipient mice

Behnke, Jerzy M.; Williams, Diana; Hannah, John; Pritchard, David

doi:10.1017/s0031182000057991

Cited by 17 publications

(11 citation statements)

References 27 publications

(35 reference statements)

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“…Infection can lead to anaemia, loss of condition and death of the host, especially lambs [1, 2]. Highly complex and specialized host-parasite interactions are involved in the evasion of host immune response by nematode parasites that help in their prolonged survival with in the mammalian hosts [3, 4]. To survive in the face of the host's immune system, the parasitic nematodes produce an array of molecules, located on the cuticle surface and/or being released as excretory secretory proteins (ESP), during host-parasite interface [5, 6].…”

Section: Introductionmentioning

confidence: 99%

Characterization of a secreted macrophage migration inhibitory factor homologue of the parasitic nematode Haemonchus Contortus acting at the parasite-host cell interface

Wang

et al. 2017

Oncotarget

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Modulation and suppression of the immune response of the host by nematode parasites have been reported extensively and the migration inhibitory factor (MIF) is identified as one of the major immunomodulator. In the present study, we cloned and produced recombinant MIF protein from the small ruminant’s nematode parasite Haemonchus contortus (rHCMIF-1), and investigated its immunomodulatory effects on goat monocyte. Enzymatic assays indicated that rHCMIF-1 possessed tautomerase activity. Immunohistochemical test demonstrated that the native HCMIF-1 protein was predominantly localized at the body surface and internal surface of the worm’s gut. We demonstrated that rHCMIF-1 could be distinguished by antisera from goats experimentally infected with H. contortus and could bind by goat monocytes. The immunomodulatory effects of HCMIF-1 on cytokine secretion, MHC molecule expression, NO production and phagocytosis were observed by co-incubation of rHCMIF-1 with goat monocytes. The results showed that the interaction of rHCMIF-1 decreased the production of TNF-α, IL-1β and IL-12p40, where as, it significantly increased the secretion of IL-10 and TGF β in goat monocytes. After rHCMIF-1 exposure, the expression of MHC-II on goat monocytes was inhibited. Moreover, rHCMIF-1 could down-regulate the LPS induced NO production of goat monocytes. Phagocytotic assay by FITC-dextran internalization showed that rHCMIF-1 could inhibit the phagocytosis of goat monocytes. Our findings provided potential targetas immunoregulator, and will be helpful to elucidate the molecular basis of host–parasite interactions and search for new potential protein as vaccine and drug target candidate.

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Section: Introductionmentioning

confidence: 99%

Characterization of a secreted macrophage migration inhibitory factor homologue of the parasitic nematode Haemonchus Contortus acting at the parasite-host cell interface

Wang

et al. 2017

Oncotarget

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“…The IgG1 isotype is a very abundant immunoglobin produced during H. bakeri infection and nearly 50% of total serum IgG1 is specific to Heligmosomoides during infection (Pritchard et al ., 1983). Adult worms produce immunomodulatory factors to dampen the effects of the host immune response (Telford et al ., 1998; Rzepecka et al ., 2006), allowing susceptible mouse strains to remain infected for many months, depending on infection intensity (Behnke et al ., 1987). Although all mice will eventually clear a primary H. bakeri infection, susceptibility to chronic H. bakeri infection varies among mouse strains.…”

Section: Introductionmentioning

confidence: 99%

Chronic calorie restriction increases susceptibility of laboratory mice (Mus musculus) to a primary intestinal parasite infection

Kristan

2007

Aging Cell

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SummaryLong-term calorie restriction (CR) has numerous benefits; however, effects of CR on susceptibility to intact pathogens are not well understood. Because CR enhances immune function of laboratory mice ( Mus musculus ), it was hypothesized that mice subjected to CR would be less susceptible to experimental infections of the intestinal parasite Heligmosomoides bakeri . Furthermore, because H. bakeri must combat a greater host immune response by CR mice compared to fully fed mice, it also was also hypothesized that (i) worms living in CR hosts would have lower reproduction than worms from ad libitum -fed mice, and (ii) CR mice would have a more female-biased sex ratio as male worms may be more vulnerable to host immune response than female worms. Mice were subjected to CR for 6.7 months and were then infected with H. bakeri for one additional month. As expected, CR mice had equal or enhanced immune response (eosinophils and immunoglobin G1 production) to H. bakeri infection compared to ad libitum -fed mice, and CR mice harbored a more femalebiased sex ratio than ad libitum -fed mice. Contrary to predictions, CR mice had more worms than ad libitum -fed mice and the worms from CR mice produced more eggs than worms from ad libitum -fed mice. These data indicate that, despite the evidence that long-term CR enhances traditional measures of immune function, CR may actually increase susceptibility to intact parasite infection. Furthermore, changes in worm reproduction and differential survival of male vs. female worms may influence hostparasite transmission dynamics during long-term host CR.

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“…The parasitic nematode Haemonchus contortus typically occurs in small ruminants and is an especially severe threat to the production and health of goats and sheep in warm and tropical temperate zones [1]. Specialized and highly complex interactions between parasitic nematodes and their hosts involve the parasite escaping from immunological responses, which allows the parasite to survive within its host [2,3]. In order to evade the host immune system, a variety of molecules produced by nematode parasites are released as excretory/secretory proteins and/or located on the cuticle surface at the host-parasite interface [4,5].…”

Section: Introductionmentioning

confidence: 99%

Characterization of a rhodanese homologue from Haemonchus contortus and its immune-modulatory effects on goat immune cells in vitro

et al. 2020

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Background Modulation of the host immune response by nematode parasites has been widely reported. Rhodaneses (thiosulfate: cyanide sulfurtransferases) are present in a wide range of organisms, such as archaea, bacteria, fungi, plants and animals. Previously, it was reported that a rhodanese homologue could be bound by goat peripheral blood mononuclear cells (PBMCs) in vivo. Methods In the present study, we cloned and produced a recombinant rhodanese protein originating from Haemonchus contortus (rHCRD), a parasitic nematode of small ruminants. rHCRD was co-incubated with goat PBMCs to assess its immunomodulatory effects on proliferation, apoptosis and cytokine secretion. Results We verified that the natural HCRD protein localized predominantly to the bowel wall and body surface of the parasite. We further demonstrated that serum produced by goats artificially infected with H. contortus successfully recognized rHCRD, which bound to goat PBMCs. rHCRD suppressed proliferation of goat PBMCs stimulated by concanavalin A but did not induce apoptosis in goat PBMCs. The production of TNF-α and IFN-γ decreased significantly, whereas secretion of IL-10 and TGF-β1 increased, in goat PBMCs after exposure to rHCRD. rHCRD also inhibited phagocytosis by goat monocytes. Moreover, rHCRD downregulated the expression of major histocompatibility complex (MHC)-II on goat monocytes in a dose-dependent manner, but did not alter MHC-I expression. Conclusions These results propose a possible immunomodulatory target that may help illuminate the interactions between parasites and their hosts at the molecular level and reveal innovative protein species as candidate drug and vaccine targets.

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Immunological relationships during primary infection with Heligmosomoides polygyrus (Nematospiroides dubius): the capacity of adult worms to survive following transplantation to recipient mice

Cited by 17 publications

References 27 publications

Characterization of a secreted macrophage migration inhibitory factor homologue of the parasitic nematode Haemonchus Contortus acting at the parasite-host cell interface

Characterization of a secreted macrophage migration inhibitory factor homologue of the parasitic nematode Haemonchus Contortus acting at the parasite-host cell interface

Chronic calorie restriction increases susceptibility of laboratory mice (Mus musculus) to a primary intestinal parasite infection

Characterization of a rhodanese homologue from Haemonchus contortus and its immune-modulatory effects on goat immune cells in vitro

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