Passionfruit ringspot virus isolated from Adenia lobata in Ivory Coast

Abstract

During a study on passionfruit viruses in Ivory Coast, a survey for wild Passifloraceae in the neighbourhood of the ORSTOM, revealed that Adenia lobata was very often present in het forest border. Plants showing mosaic symptoms in their leaves were first found in 1970. Check inoculations on Passiflora edulis var. flaviearpa andP.foetida caused the same symptoms in these test plants as passionfruit ringspot virus (PRV)did (De Wijs, 1974). A. lobata plants with less obvious symptoms and even without symptoms gave the same reaction. One plant was chosen for further indentification of the virus involved. From the beginning of the study PRV was used for comparison with the Adenia virus (AV).The preparation of inoculum, determination of the in vitro properties, aphid transmission experiments, purification of the AV from A. lobata, length measurements of the virus particles and serological testing of the crude juice of P. edulis were done as described earlier (De Wijs, 1974). Purified virus preparations were examined in an electron microscope after contrasting with neutral 2 ~ (w/v) phosphotungstate. For antigen from A. lobata the crude sap had to be clarified more thoroughly to avoid aspecific reactions with the available antiserum (homologous titre 4096) against PRV. Crude sap of A. lobata had to be emulsified with an equal volume of chloroform for 30 min and clarified by low-speed centrifugation, 10 min. at 12,000 g, in the SS 34 rotor of a Sorvall RC2B refrigerated centrifuge. The supernatant was deep frozen at -18 ~ for 18 h and once more clarified by low-speed centrifugation after thawing to get rid of fraction 1 protein (Van Regenmortel, 1964). Antigen was then concentrated by high-speed centrifugafion in a Spinco L50 preparative centrifuge: 150 min at 54,000 g in the R 30 rotor. The pellets were resuspended in a 0.9 % NaC1 solution and clarified by low-speed centrifugation. Host range and symptoms.

Virus and Phytoplasma Diseases of Passion Fruit

“…Flavicarpa in the Ivory Coast, shriveled fruits with a reduced pulp cavity were also found, however, without "woodiness" (De Wijs, 1974a). De Wijs and Mobach (1975) isolated passion ringspot virus from Adenia lobata in Ivory Coast.…”

“…in Ivory Coast (De Wijs, 1975). De Wijs and Mobach (1975) found a virus on A. lobata causing ringspot symptoms. The causal virus is passion fruit ringspot virus.…”

The distribution of passionfruit ringspot virus in its main host plants in Ivory Coast

“…Adenia lobata, a member of the Passifloraceae and indigenous to West Africa (De Wilde, 1971), harbours the virus (De Wijs and Mobach, 1975). A survey has now been undertaken in Ivory Coast for the virus in wild Passifloraceae, especially Adenia spp.…”