Parvoviridae: Second Report

Bachmann, Peter; Hoggan, David; Kurstak, Edouard; Melnick, Joseph L.; Pereira, H. G.; Tattersall, Peter; Vago, C.

doi:10.1159/000149041

Cited by 39 publications

(20 citation statements)

References 2 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Furthermore, in the morphogenesis of some picornaviruses, a wide range of morphogenic intermediates has been detected with sedimentation coefficients of 45, 70, 80,90, 125, 130, 160 and 220S [44][45][46], During the initial studies of the biophysical characteristics of HAV, the observations of multiple buoyant densities and sedimentation coefficients resulted in some confusion about the classification of HAV. The instability of heavy density (1.38-1.41 g/cm3) particles, the stability of light density (1.32-1.34 g/cm3) particles, and the appearance of 'empty' par ticles with a mean density of 1.29 g/cm3 were consistent with HAV being either a picornavirus [41,43,46] or a parvovirus [47][48][49], Its acid, ether and heat resistance (60° for 1 h) [50] was consistent with these suggestions, while its size (27-32 nm) was more in keeping with that expected of a picornavirus (26-34 nm) [5,7]. Definitive classification of HAV was achiev able only after it became possible to charac terize the capsid polypeptide and the nucleic acid.…”

Section: Characterization Of Hepatitis a Virusmentioning

confidence: 55%

“…Analysis of the laboratory grade BSA (track C) revealed only the presence of p66 and p68. and differed completely from the profile typi cally seen in the family Parvoviridae [7]. A fourth low-molecular-weight polypeptide, which is common to picornaviruses, was not detected probably because of the insensitivity of the Coomassie blue staining technique and the known lability of the picornaviral VP4 [5,25,26], In a later study, Coulepis et al [30] radiolabeled purified HAV with 1251 and then ana- lyzed it by discontinuous SDS-PAGE in a slab gel system.…”

Section: Biochemical Characterization O F Ha V (I) Polypeptide Composmentioning

confidence: 95%

“…The particles were morphologically similar to members of the small RNA-containing picornaviruscs [4,5] and the DNAcontaining parvoviruses [6,7].…”

mentioning

confidence: 99%

See 2 more Smart Citations

Biophysical and Biochemical Characterization of Hepatitis A Virus

et al. 1982

View full text Add to dashboard Cite

Biophysical and biochemical analysis of hepatitis A virus has shown it to be a 27- to 32-nm icosahedral particle with 32 capsomers. The mature virion has a buoyant density of 1.33–1.34 g/cm³, a sedimentation coefficient of 156–160S, and is composed of four polypeptides with molecular weights of 30,000–33,000 (VPl), 24,000–27,000 (VP2), 21,000–23,000 (VP3), and 7,000–14,000 (VP4). The genome of hepatitis A virus consists of a single piece of single-stranded RNA which sediments at 32–35S and has a buoyant density of 1.64 g/cm³. The molecular weight of RNA is 2.25 × 10⁶ when measured under nondenaturing conditions and 2.8 × 10⁶ when measured under fully denaturing conditions. The genome contains a 40–80 nucleotide sequence of polyadenylic acid and is capable of infecting cell cultures. These findings, together with the observation that the virion is stable at pH 3.0 and resistant to ether and a temperature of 60° for 1 h, indicate that hepatitis A virus should now be classified as an Entero-virus within the family Picornaviridae.

show abstract

Section: Characterization Of Hepatitis a Virusmentioning

confidence: 55%

Section: Biochemical Characterization O F Ha V (I) Polypeptide Composmentioning

confidence: 95%

See 1 more Smart Citation

Biophysical and Biochemical Characterization of Hepatitis A Virus

et al. 1982

View full text Add to dashboard Cite

show abstract

“…

The second and most recent report on the taxonomy of Parvoviridae [6] was submitted for publication in May 1978. Since then, a vast amount of additional information con cerning the characteristics of these small and exceedingly stable viruses has accumulated.

…”

mentioning

confidence: 99%

Characteristics and Taxonomy of <i>Parvoviridae</i>

Siegl¹,

Bates²,

Berns³

et al. 1985

Intervirology

Self Cite

280

144

View full text Add to dashboard Cite

“…Although inclusion bodies were not demonstrated in the tissues of the intestinal tract, histological studies showed ~ mild intestinal cetarrh. Parvm~uses, in general, grow effectively in rapidly dividing cells and in rapidly-replicating tissues (2). Rabbit parvovirus may grow in epithelial cells of the intestinal tract.…”

Section: Diseussionmentioning

confidence: 99%

Experimental infection of young rabbits with rabbit parvovirus

Matsunaga

Chino

1981

Archives of Virology

View full text Add to dashboard Cite

A newly isolated parvovirus of rabbits (F-7-9 strain) was inoculated into young female rabbits by the oral or intravenous route. Virus was recovered from the feces 3 to 13 days after inoculation. Viremia was demonstrated in orally inoculated rabbits. The virus was also isolated from tissue extracts of liver, pancreas, spleen, small intestine, appendix and mesenteric lymph node on days 10 and/or 14, and from the small intestine on day 30 after inoculation. Hemagglutination inhibiting antibody appeared in the serum after 8 days and neutralizing antibody was detected later. An 11S neutralizing antibody was demonstrated in fecal extracts between 15 and 30 days after inoculation. Infected rabbits showed very mild clinical signs of listlessness and inappetence lasting for a few days. Histologically, mild to moderate catarrhal enteritis was observed in the small intestine.

show abstract

Parvoviridae: Second Report

Cited by 39 publications

References 2 publications

Biophysical and Biochemical Characterization of Hepatitis A Virus

Biophysical and Biochemical Characterization of Hepatitis A Virus

Characteristics and Taxonomy of <i>Parvoviridae</i>

Experimental infection of young rabbits with rabbit parvovirus

Contact Info

Product

Resources

About