Partitioning of whey proteins, bovine serum albumin and porcine insulin in aqueous two-phase systems

Alves, José Guilherme Lembi Ferreira; Chumpitaz, Lucy D. A.; Silva, L. H. M. Da; Franco, Telma Teixeira; Meirelles, Antônio J. A.

doi:10.1016/s0378-4347(00)00111-0

Cited by 62 publications

(47 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…65%) produced by recombinant E. coli was reported using an one-stage ATPS process [16]. These reports and others [2][3][4][5][6][7][8][9][10][11][12][13][14][15] clearly demonstrated the benefits of ATPS processes at bench scale for the recovery of products. Furthermore, the type of proteins products that have been recovered with acceptable process yield (i.e.…”

Section: Atps Processes For the Recovery Of Proteinsmentioning

confidence: 74%

“…A critical analysis of reports [2][3][4][5][6][7][8][9][10][11][12][13][14][15] dealing with the use of ATPS in bioproducts recovery exhibited that the application of ATPS for the recovery of proteins has resulted in processes designed as primary purification operations. Such processes have been characterised by single or multi-staged operations.…”

Section: Atps Processes For the Recovery Of Proteinsmentioning

confidence: 99%

See 1 more Smart Citation

Practical application of aqueous two-phase partition to process development for the recovery of biological products

Rito‐Palomares

2004

Journal of Chromatography B

311

180

View full text Add to dashboard Cite

Section: Atps Processes For the Recovery Of Proteinsmentioning

confidence: 74%

Section: Atps Processes For the Recovery Of Proteinsmentioning

confidence: 99%

Practical application of aqueous two-phase partition to process development for the recovery of biological products

Rito‐Palomares

2004

Journal of Chromatography B

311

180

View full text Add to dashboard Cite

“…For instance, PEG + sodium citrate ATPS were used to partition glycomacropeptide with a K P of 33.41 . PEG 1500 + potassium phosphate ATPS were used to partition ␣-lactalbumin and ␤-lactoglobulin with K P values of 12.614 and 0.063 (Rojas et al, 2004), and 8.0 and 0.056 (Alves et al, 2000), respectively. PEG 1500 + potassium phosphate ATPS were used to partition BSA, ␣-lactalbumin, and ␤-lactoglobulin, with a K P of 10, 27, and 0.07, respectively (Capezio et al, 2005).…”

Section: Partition Coefficientmentioning

confidence: 99%

“…The two phases consist primarily of water (between 70% and 90%) and provide an adequate environment with favourable conditions for biomolecule distribution, in addition to a low cost of implementation and ease of scale-up (Albertsson, 1986;Coimbra and Teixeira, 2010;Zaslavsky, 1995). ATPS are used to concentrate and purify whey proteins (Alves et al, 2000;Anandharamakrishnan et al, 2005;Capezio et al, 2005;Silva et al, 2009). These proteins have high biological and functional value and confer technological and nutritional properties to food products.…”

Section: Introductionmentioning

confidence: 99%

Partitioning of bovine lactoferrin in aqueous two-phase system containing poly(ethylene glycol) and sodium citrate

Costa

Coimbra

Ferreira

et al. 2015

Food and Bioproducts Processing

View full text Add to dashboard Cite

ATPS Molar mass PEG pH Phase diagram Whey protein a b s t r a c tThe partitioning of the whey protein lactoferrin, which is an iron transporter glycoprotein, in an aqueous two-phase system composed of poly(ethylene glycol) (PEG) and sodium citrate was evaluated. Equilibrium data at 25 • C were determined for each system studied using PEG with a molar mass of 1000 and 4000 g mol −1 at pH values of 5.5, 6.5, and 7.5. An increase in the molar mass of the polymer promoted the expansion of the two-phase region and caused the migration of the lactoferrin to the salt-rich bottom phase. An increase in pH also led to the expansion of the biphasic region. However, changing the pH over the tested range slightly affected protein partitioning. Lactoferrin recovery percentages greater than 94% were observed for all of the systems evaluated. The results indicated that lactoferrin can be successfully partitioned in an aqueous two-phase system formed of 14% (w/w) PEG and 10% (w/w) sodium citrate at pH 5.5 and 25 • C. The protein was concentrated 1000-fold in the salt-rich bottom phase in this system. of purity and recovery; and (iii) be efficient, effective, and economical for large-scale processes. Aqueous two-phase systems (ATPS) are an alternative for separating, concentrating, and purifying biologically active molecules such as proteins in complex mixtures. ATPS are formed by mixing either two polymers (such as poly(ethylene glycol)/dextran) or one polymer http://dx.

show abstract

“…Purification is troublesome because of system complexity and the need to retain biological activity. Usually, biological material has been purified by precipitation with salts or organic solvents, and by using various chromatographic techniques, all of them having enormous difficulties in large-scale applications (Alves et al, 2000). Besides, according to Diamond and Hsu (1992), 50-90% of the biological products production costs are determined by the purification strategy.…”

Section: Introductionmentioning

confidence: 99%

Large scale purification of Clostridium perfringens toxins: a review

Cavalcanti¹,

Porto²,

Porto³

et al. 2004

Rev. Bras. Cienc. Farm.

View full text Add to dashboard Cite

Clostridium perfringens, a Gram-positive anaerobic bacterium, is widespread in the environment and commonly found in the intestines of animals, including humans. C. perfringens strains are classified into five toxinotypes (A, B, C, D and E) based on the production of four major toxins (α, β, ε, ι). However the toxins (theta, delta, lambda and enterotoxin) are also synthesized by C. perfringens strain. Many attempts to purify the toxins produced by C. perfringens have been proposed. In this review we discuss the purification methods used to isolate toxins from C. perfringens reported in last four decades.

show abstract

Partitioning of whey proteins, bovine serum albumin and porcine insulin in aqueous two-phase systems

Cited by 62 publications

References 8 publications

Practical application of aqueous two-phase partition to process development for the recovery of biological products

Practical application of aqueous two-phase partition to process development for the recovery of biological products

Partitioning of bovine lactoferrin in aqueous two-phase system containing poly(ethylene glycol) and sodium citrate

Large scale purification of Clostridium perfringens toxins: a review

Contact Info

Product

Resources

About