Practical application of aqueous two-phase partition to process development for the recovery of biological products

Extraction and Purification of Bioproducts and Nanoparticles using Aqueous Two‐Phase Systems Strategies

“…There are three main types of ATPS: (1) polymer-salt, (2) polymerpolymer, and (3) ATPS constructed with alternative compounds, e.g., ethylene oxide and propylene oxide copolymers (EOPO), hydroxylpropyl starch (HPS), iminoadiacetic acid (IAA), etc. [3]. Of the polymer-salt systems uses, polyethylene glycol (PEG)-potassium phosphate, is particularly preferred due to important advantages, including extensive characterization, low cost, and a wide range of applications [3].…”

“…[3]. Of the polymer-salt systems uses, polyethylene glycol (PEG)-potassium phosphate, is particularly preferred due to important advantages, including extensive characterization, low cost, and a wide range of applications [3]. The first studies involving ATPS date from the late 1950's and early 1960's, when Albertsson [4] demonstrated the great potential of this technique for the primary recovery of biological compounds.…”

“…The first studies involving ATPS date from the late 1950's and early 1960's, when Albertsson [4] demonstrated the great potential of this technique for the primary recovery of biological compounds. Furthermore, process integration and intensification can be achieved using strategies based upon ATPS resulting in optimized processes that are easy to scale up [1][2][3]. Process integration results when one single unit operation can achieve the same process objective of two or more discrete processing stages.…”

Continuous purification of antibodies from cell culture supernatant with aqueous two‐phase systems: From concept to process

“…This liquid-liquid extraction (LLE) technology has been successfully reported in recent times as a primary stage unit operation in the downstream processing of several biological products including therapeutic proteins, such as antibodies [4,10,11,[15][16][17][18][19][20][21][22][23][24]. Its technical feasibility at large scale has been, however, demonstrated for the downstream processing of just few biological products [25][26][27]. This may be attributed, on one hand, to the limited knowledge of the mechanism of solute partitioning in ATPS, and in another hand, to the disadvantages of batch operations as well as to the difficulties associated with the implementation of ATPE processes in a continuous mode of operation [25].…”

“…Its technical feasibility at large scale has been, however, demonstrated for the downstream processing of just few biological products [25][26][27]. This may be attributed, on one hand, to the limited knowledge of the mechanism of solute partitioning in ATPS, and in another hand, to the disadvantages of batch operations as well as to the difficulties associated with the implementation of ATPE processes in a continuous mode of operation [25]. Indeed, in most of the few ATPE processes described in the literature, the equipment assembly (agitated vessel + centrifuge) used provides only one theoretical stage and is expensive at large scale [28].…”