Partitioning of doxorubicin into Langmuir and Langmuir–Blodgett biomimetic mixed monolayers: Electrochemical and spectroscopic studies

Nieciecka, Dorota; Królikowska, Agata; Joniec, Aleksandra; Krysiński, Paweł

doi:10.1016/j.jelechem.2013.03.004

Cited by 9 publications

(17 citation statements)

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“…The resulting changes are quite similar to those reported by us for the case of mixed monolayers of the same molecules, containing additionally a small amount of octadecanethiol anchor molecules. 30 As reported previously, the presence of DOX in the subphase causes an increase of mean area per molecule for all monolayers studied. Moreover, the change of the slope of the isotherms shows the fluidizing effect of doxorubicin on these films.…”

Section: ■ Results and Discussionsupporting

confidence: 71%

“…The functional form of the L-B isotherms contains information on film quality, as we have discussed previously. 29,30 First, we analyzed the Langmuir isotherms for the case of C 18 NH 2 , DHP and DMPC monolayers in the absence and presence of 1 × 10 −5 M DOX present in the subphase (Figure 1a−c). It is evident from this figure that DOX affects the pressure/area isotherms of these amphiphiles differently.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

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Interactions of Doxorubicin with Organized Interfacial Assemblies. 1. Electrochemical Characterization

et al. 2013

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Doxorubicin is an anthracycline that has found wide use as a chemotherapeutic agent, with the primary target of its action being nuclear DNA. Despite the large body of knowledge on this family of compounds, the mechanism of doxorubicin penetration through the cellular or nuclear membrane remains understood to a limited extent. The plasma membrane acts as a barrier to the permeation of polar molecules, and this effect is mainly due to the hydrophobicity of membrane interior. The partitioning of DOX molecules into the lipid bilayer must thus be the basis for its passive transport across the biological membrane and therefore a key area of research activity lies in understanding how the structure of the anthracycline influences its interactions with amphiphilic interfaces. We have studied interactions between doxorubicin and Langmuir/Langmuir-Blodgett monomolecular films of octadecylamine (C18NH2), dihexadecylphosphate (DHP) and 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC), and DMPC bilayer films (Langmuir-Schaeffer) on a polycrystalline gold surface using ellipsometry, cyclic voltammetry, electrochemical impedance spectroscopy, and quartz crystal microbalance measurements. For all biomimetic films there is a substantial interaction between doxorubicin and the interface, and the extent of this interaction depends on the hydrophobic/hydrophilic properties of the film formed and its organization.

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Section: ■ Results and Discussionsupporting

confidence: 71%

Section: ■ Results and Discussionmentioning

confidence: 99%

Interactions of Doxorubicin with Organized Interfacial Assemblies. 1. Electrochemical Characterization

et al. 2013

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“…When referring to the lipid monolayer, it is inevitable to mention the Langmuir trough, where various parameters such as lipid composition, subphase, and temperature can be chosen and modified in order to mimic the biological conditions. The Langmuir trough or Langmuir balance provides information about the characteristics of a monolayer on the aqueous surface by measuring the changes in surface tension upon compressing the monolayer.Lipid monolayers are formed at the air-water interface in a Langmuir trough and offer the possibility of using methodologies that are able to detect membrane drug penetration through Langmuir monolayer techniques[62] and the drug's effect on lipid monolayer structural properties by Brewster angle microscopy (BAM) [63](Figure 3). More detailed information about the application of Langmuir monolayer associated techniques to the study of interactions between biomolecules and cell membranes can be found in the following reviews[64][65][66].…”

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confidence: 99%

Biophysics in cancer: The relevance of drug-membrane interaction studies

Alves

Ribeiro

Nunes

et al. 2016

Biochimica et Biophysica Acta (BBA) - Biomembranes

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Lipidomics has been proving that membrane lipids play a crucial role in several cell functions and are involved in several pathologies, including cancer. In fact, beyond a scaffold where proteins and other components are embedded, the cell membrane can also act as a barrier or a target for anticancer drugs. From this point of view, the development of new chemotherapeutic agents should also take into account the role of the membrane in their activity. This Review aims to highlight the importance of anticancer drug-membrane interactions as a powerful strategy to improve cancer therapy. Biophysical techniques emerge, therefore, as essential tools to unveil such interactions.

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“…Electrostatic interactions are certainly important in attracting drug molecules to the membrane; however, in the subsequent penetration process, hydrophobic interactions play an important role. This was evidenced for doxorubicin [211] and for dipyridamole and its derivatives, which -due to their dual application -in anticancer therapy (as coactivator of the anti-tumor effects of other drugs) and in coronary heart disease, are discussed later, with cardiac drugs.…”

Section: Accepted Manuscriptmentioning

confidence: 92%

Interactions of bioactive molecules & nanomaterials with Langmuir monolayers as cell membrane models

et al. 2015

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Partitioning of doxorubicin into Langmuir and Langmuir–Blodgett biomimetic mixed monolayers: Electrochemical and spectroscopic studies

Cited by 9 publications

References 46 publications

Interactions of Doxorubicin with Organized Interfacial Assemblies. 1. Electrochemical Characterization

Interactions of Doxorubicin with Organized Interfacial Assemblies. 1. Electrochemical Characterization

Biophysics in cancer: The relevance of drug-membrane interaction studies

Interactions of bioactive molecules & nanomaterials with Langmuir monolayers as cell membrane models

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