Partitioning and extraction protease from Aspergillus tamarii URM4634 using PEG-citrate aqueous two-phase systems

Silva, Osmar Soares da; Gomes, Matheus Henrique Gouveia; Oliveira, Rodrigo Lira de; Converti, Attílio; Porto, Tatiana Souza

doi:10.1016/j.bcab.2016.12.012

Cited by 29 publications

(12 citation statements)

References 39 publications

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“…Alkaline protease is the best choice to remove sericin while not attacking the fiber. It has been proven that fiber break is not amenable, and silk threads are found to be much stronger than when previous traditional treatments were used (Yadav et al, 2011; da Silva et al, 2017; Radha et al, 2017).…”

Section: Silk Degummingmentioning

confidence: 99%

Microbial Proteases Applications

Razzaq

Shamsi

Ali³

et al. 2019

Front. Bioeng. Biotechnol.

347

182

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The use of chemicals around the globe in different industries has increased tremendously, affecting the health of people. The modern world intends to replace these noxious chemicals with environmental friendly products for the betterment of life on the planet. Establishing enzymatic processes in spite of chemical processes has been a prime objective of scientists. Various enzymes, specifically microbial proteases, are the most essentially used in different corporate sectors, such as textile, detergent, leather, feed, waste, and others. Proteases with respect to physiological and commercial roles hold a pivotal position. As they are performing synthetic and degradative functions, proteases are found ubiquitously, such as in plants, animals, and microbes. Among different producers of proteases, Bacillus sp. are mostly commercially exploited microbes for proteases. Proteases are successfully considered as an alternative to chemicals and an eco-friendly indicator for nature or the surroundings. The evolutionary relationship among acidic, neutral, and alkaline proteases has been analyzed based on their protein sequences, but there remains a lack of information that regulates the diversity in their specificity. Researchers are looking for microbial proteases as they can tolerate harsh conditions, ways to prevent autoproteolytic activity, stability in optimum pH, and substrate specificity. The current review focuses on the comparison among different proteases and the current problems faced during production and application at the industrial level. Deciphering these issues would enable us to promote microbial proteases economically and commercially around the world.

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Section: Silk Degummingmentioning

confidence: 99%

Microbial Proteases Applications

Razzaq

Shamsi

Ali³

et al. 2019

Front. Bioeng. Biotechnol.

347

182

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“…substrate concentration, while decreasing that of the less-influencing or even negativelyinfluencing one, i.e. yeast extract concentration [39].…”

Section: A C C E P T E Dmentioning

confidence: 99%

Optimized production of Aspergillus aculeatus URM4953 polygalacturonases for pectin hydrolysis in hog plum (Spondias mombin L.) juice

Silva

França

Mélo

et al. 2019

Process Biochemistry

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“…ATPE is typically formed by mixing two hydrophilic polymers such as PEG (polyethylene glycol) and dextran or a polymer and a salt, such as PEG and potassium phosphate, in which phase separation occurs spontaneously apart from the critical concentrations of phase components (Silva et al, 2017;Ferreira et al, 2011). Under favorable conditions, the target protein tends to be partitioned into the top phase, which is less dense, less polar, for example, the PEG phase, while contaminant proteins are found in the denser, more polar and hydrophilic one, i.e., the dextran or salty phase, and can be removed through centrifugation (Arshad et al, 2014).…”

Section: Aqueous Two-phase Extraction (Atpe)mentioning

confidence: 99%

“…The main reason for this could be attributed to the poor knowledge about the mechanisms involved in the partitioning equilibrium of macromolecules and, consequently, the lack of a comprehensive theory able to predict the experimental trends (Rocha and Nerli, 2013). Also, high concentrations of salts in wastewater are known to cause environmental problems (Silva et al, 2017). Rabelo et al (2004) studied the purification of bromelain using an ATPE system formed by thermoseparating copolymers: PEO (polyethylene oxide)-PPO (polypropylene oxide)-PEO (polyethylene oxide) and the best result (purification factor of 1.25) was achieved when using the conditions: copolymer with 10% EO (m/m) and molecular mass of 2000 g/ mol, copolymer concentration of 5% (m/m) and temperature 5ºC above the cloud point (25 ºC in this case), pH 6.0 and salt concentration of 15 mM.…”

Section: Aqueous Two-phase Extraction (Atpe)mentioning

confidence: 99%

Bromelain Separation and Purification Processes From Pineapple Extract

Abreu

Figueiredo

2019

Braz. J. Chem. Eng.

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The concern about the effective use of agro-industrial residues from the food industry has increased. Bromelain is an enzyme mainly obtained from the pineapple plant (A. comosus), including its parts normally considered as waste. It is a high value-added product with a wide variety of applications such as in food, cosmetic and pharmaceutical industries. Many approaches have been used to purify this enzyme, but an efficient method to produce highly active and pure bromelain is still highly desirable. The investigated methods include ion exchange chromatography, precipitation, an aqueous two-phase system as well as affinity and filtration membranes. This paper gives an overview about the advantages and limitations of each one. Chronologic results and advances for these separation processes are also shown. The major tendency is the use of combined processes, such as hybrid or integrated ones.

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Partitioning and extraction protease from Aspergillus tamarii URM4634 using PEG-citrate aqueous two-phase systems

Cited by 29 publications

References 39 publications

Microbial Proteases Applications

Microbial Proteases Applications

Optimized production of Aspergillus aculeatus URM4953 polygalacturonases for pectin hydrolysis in hog plum (Spondias mombin L.) juice

Bromelain Separation and Purification Processes From Pineapple Extract

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