Particulate lipid class composition of three marine phytoplankters Chaetoceros gracilis, Isochrysis galbana (Tahiti) and Dunaliella tertiolecta grown in batch culture

Lombardi, AT; Wangersky, Peter J.

doi:10.1007/bf00007853

Cited by 63 publications

(35 citation statements)

References 20 publications

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“…Afify et al (2010) found a similar value, 9.2% dw of lipid for the filamentous cyanobacterium Spirulina platensis. There are many reports in the literature of eukaryotic microalgae that responded to nitrogen starvation with expressive increases in lipid production (Lombardi & Wangersky, 1995;Guevara et al, 2005;Chen et al, 2011;Karatay & Dönmez, 2011), contrasting with our study.…”

Section: Gross Chemical Compositioncontrasting

confidence: 99%

An assessment of the usefulness of the cyanobacterium Synechococcus subsalsus as a source of biomass for biofuel production

Setta¹,

Barbarino²,

Passos³

et al. 2014

lajar

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ABSTRACT. Nowadays algal biofuels are considered one of the most promising solutions of global energy crisis and climate change for the years to come. By manipulation of the culture conditions, many algal species can be induced to accumulate high concentrations of particular biomolecules and can be directed to the desired output for each fuel. In this context, the present study involved the assessment of the effects of CO 2 availability and nitrogen starvation on growth and chemical composition of the cyanobacterium Synechococcus subsalsus, testing a fast-growing native strain. The control experiments were performed with Conway culture medium in 12-day batch cultures, in 6-liter flasks and 12 h photoperiod, with addition of 2 L min -1 filtered air to each flask. Other two experimental conditions were also tested: (i) the placement into the cultures of additional dissolved nutrients except nitrogen, one week after the start of growth (N-), and (ii) the input of pure CO 2 into the flasks from the 5 th day of growth (C+). In all cultures, daily cell counts were done throughout the cultivation, as well as measurements of pH and cell biovolumes. Maximum cell yield were found in N-experiments, while cell yields of C+ and control were similar. Dissolved nitrogen was exhausted before the end of the experiments, but dissolved phosphorus was not totally consumed. Protein and chlorophyll-a concentrations decreased from the exponential to the stationary growth phase of all experiments, except for protein in the control. In all experiments, carbohydrate, lipid and total carotenoid increased from the exponential to the stationary growth phase, as an effect of nitrogen limitation. Increments in carbohydrate concentrations were remarkable, achieving more than 42% of the dry weight (dw), but concentrations of lipid were always lower than 13% dw. The addition of pure CO 2 did not cause a significant increase in biomass of S. subsalsus nor generated more lipid and carbohydrate than the other treatments. Nitrogen starvation caused an intense accumulation of carbohydrate, but the increments of lipid were small. Despite the fast growth, the cyanobacterium S. subsalsus has a virtually null potential for biodiesel production, given its low lipid concentrations. The high concentrations of carbohydrate combined with fast growth point to the potential use of this species as raw material for other possible biotechnological processes, after a demonstration of technical and economic viability. Keywords: biodiesel, biomass, cultivation, lipid, microalgae, cyanobacteria, productivity.Evaluación de la utilidad de la cianobacteria marina Synechococcus subsalsus como fuente de biomasa para la producción de biocombustibles RESUMEN. Actualmente los biocombustibles elaborados con algas se consideran una de las soluciones más prometedoras de la crisis energética mundial y el cambio climático para los años venideros. Mediante la manipulación de las condiciones de cultivo, muchas especies de algas pueden ser inducidas para acumular altas concentracione...

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Section: Gross Chemical Compositioncontrasting

confidence: 99%

An assessment of the usefulness of the cyanobacterium Synechococcus subsalsus as a source of biomass for biofuel production

Setta¹,

Barbarino²,

Passos³

et al. 2014

lajar

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“…Probably, this state represents an`optimum' for P. tricornutum cells as suggested by the fact that biomass productivity reached a maximum at this dilution rate; the biomass productivity decreased when culture was moved in either direction from this optimum point (data not shown). A reduction in polar lipids and an increase in TAG are usually associated with culture ageing (A Â lvarez Cobelas, 1989;Hodgson et al, 1991;Dunstan et al, 1993;Lombardi and Wangersky, 1995;Brown et al, 1996), however, the speci®c response may be dierent in dierent microalgae (Lombardi and Wangersky, 1995), or the response may depend on the steady state reached as shown in this work. When dilution rate increases (i.e.…”

Section: Lipid Class Compositionmentioning

confidence: 74%

“…Increase in lipid content is usually associated with culture ageing (i.e., stationary phase) Siron et al, 1989;Hodgson et al, 1991;Ahlgren et al, 1992;Dunstan et al, 1993;Lombardi and Wangersky, 1995;Bell and Pond, 1996;LoÂ pez Alonso et al, 1998). On the other hand, nitrogen stress (Pohl and Zurheide, 1979;Shifrin and Chisholm, 1980;Parrish and Wangersky, 1987;A Â lvarez Cobelas, 1989), and nutrient limitations in general (Reitan et al, 1994) have also been positively associated with increase in lipid abundance, although the response was species-speci®c (Shifrin and Chisholm, 1980;Lombardi and Wangersky, 1995). Moreover, ageing eects may be indistinguishable from nutrient limitation eects because ageing generally involves depletion of some nutrient (A Â lvarez Cobelas, 1989) at least in batch culture which was the usual culture system in most earlier works.…”

Section: Introductionmentioning

confidence: 99%

Acyl lipid composition variation related to culture age and nitrogen concentration in continuous culture of the microalga Phaeodactylum tricornutum

et al. 2000

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The in¯uence of culture age and nitrogen concentration on the distribution of fatty acids among the dierent acyl lipid classes has been studied in continuous cultures of the microalga Phaeodactylum tricornutum. The culture age was tested in the range of 1.15±7 days, controlled by adjusting the dilution rate of fresh medium supplied. The eect of nitrogen concentration was tested from saturating conditions to starvation by modifying nitrate concentration in the fresh medium. Culture age had almost no in¯uence on the fatty acid content; 16:0, 16:3 and 20:5 increased moderately wherein the level of 16:1 decreased when the culture age decreased. Culture age had no eect on the total fatty acid content that remained around 11% of dry weight. Conversely, culture age had a greater impact on lipid classes, producing changes in amounts of triacylglycerols (TAG) which ranged between 43% and 69%, and galactolipids (GLs) that oscillated between 20% and 40%. In general, the content of polar lipids of the biomass decreased with culture age. The other factor assayed, nitrogen content, aected the fatty acid pro®le. Saturated and monounsaturated fatty acids accumulated when the nitrogen concentration was decreased. The experiments regarding the eect of nitrogen concentration on lipid species were carried out with cells of an average age of 3.5 days. A decrease of the nitrogen concentration caused the GL fraction to decrease from 21 to 12%. Conversely, both neutral lipids (NLs) and phospolipids (PLs) increased from about 73 to 79% and from 6 to 8%, respectively. In these experiments, TAG was the lipid class with the highest increase, from 69 to 75%. 7

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“…This is important for aquaculture when polar lipids contribute to the production of PUFAs through esterification in the polar lipids (Meireles et al 2003); PUFAs are known as effective growth promoters in aquaculture (Ferreira et al 2009, Hill et al 2011. AMPL is a polar lipid class that may contain 35-50% chlorophyll (Parrish and Wangersky 1987, Lombardi and Wangersky 1991, 1995, Illija et al 2009). This in fact explains the significant correlation of chlorophyll a with the AMPL lipid class.…”

Section: Resultsmentioning

confidence: 99%

Growth and biochemical composition of Chlorella vulgaris in different growth media

Chia

Lombardi

Melão

2013

An. Acad. Bras. Ciênc.

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The need for clean and low-cost algae production demands for investigations on algal physiological response under different growth conditions. In this research, we investigated the growth, biomass production and biochemical composition of Chlorella vulgaris using semi-continuous cultures employing three growth media (LC Oligo, Chu 10 and WC media). The highest cell density was obtained in LC Oligo, while the lowest in Chu medium. Chlorophyll a, carbohydrate and protein concentrations and yield were highest in Chu and LC Oligo media. Lipid class analysis showed that hydrocarbons (HC), sterol esthers (SE), free fatty acids (FFA), aliphatic alcohols (ALC), acetone mobile polar lipids (AMPL) and phospholipids (PL) concentrations and yields were highest in the Chu medium. Triglyceride (TAG) and sterol (ST) concentrations were highest in the LC Oligo medium. The results suggested that for cost effective cultivation, LC Oligo medium is the best choice among those studied, as it saved the cost of buying vitamins and EDTA associated with the other growth media, while at the same time resulted in the best growth performance and biomass production.

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Particulate lipid class composition of three marine phytoplankters Chaetoceros gracilis, Isochrysis galbana (Tahiti) and Dunaliella tertiolecta grown in batch culture

Cited by 63 publications

References 20 publications

An assessment of the usefulness of the cyanobacterium Synechococcus subsalsus as a source of biomass for biofuel production

An assessment of the usefulness of the cyanobacterium Synechococcus subsalsus as a source of biomass for biofuel production

Acyl lipid composition variation related to culture age and nitrogen concentration in continuous culture of the microalga Phaeodactylum tricornutum

Growth and biochemical composition of Chlorella vulgaris in different growth media

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