Particularities Associated With the Expression of Glial Acidic Fibrillary Protein on the Structural Components of Cerebellum of the Rats Influenced by the Food Additives Complex

Abstract

The aim: To define the degree for glial acidic fibrillary protein expression on the structural components of cerebellum of the rats in health and when rats influenced by the food additives complex. Materials and methods: In order to determine the degree of expression of the immunohistochemical marker GFAP on the structural components of the cerebellum of rats we applied immunohistochemical, morphometric and statistical methods in our study. Results: In histological specimens at the end of 1st week of observation in the gray matter of the cerebellum there occurred a gradual increase in 1.16 times of the average number of GFAP-positive cells. At the end of 4th week of the experimental study, the average number of GFAP-positive cells increased accurately (at p<0.05 compared to the control group) in 1.27 times, at the end of 8th week it has increased in 1.99 times, at the end of 12th week in 2.25, and at the end of 16th week in 2.39 times. Conclusions: The outcomes of our study are as follows the increase in the average number of GFAP-positive cells is directly related to the decrease in the average number of major neurons of the gray matter of the brain, while the fluctuations in the average number of astrocytic glia cells represent a compensatory mechanism in the recovery of gray matter neurons of the brain from neural stem cells with the subsequent development of reactive astrogliosis and, thereafter the possible development of neuropathology.

Immunohistochemical Characteristics of Structural Changes in the Cortex and Medulla of the Adrenal Glands Under the Influence of a Food Additive Complex

“…Ki-67 positive endotheliocytes were visualized in the walls of arterioles of the reticular zone of the adrenal glands of rats in the first week of the experimental study. The section of the arteriole itself remained of the correct oval shape, and the surrounding structural components were without signs of hyperhydration [12][13].…”

Structural Organization of the Adrenal Glands Medulla of Rats in Normal and After Influence of Food Additive Complex

“…This decrease is related to the influence of the components of food additives, similar to the reaction of the vascular bed to external factors [8][9]. Comparing changes in the average values of the total diameter and lumen of arterioles, capillaries, and venules of the medulla, it should be noted that these indicators correlate with each other in different periods of the experiment and reflect unidirectional morphological changes in these structures [10][11].…”

Morphological Characteristics of the Wall of Rats' Ileum When Using a Food Additives Complex

“…Therefore, the problem of the quality of food products, considering the significant number of food additives widely used in the food industry, remains highly relevant. According to literature data, food additives of a chemical nature can cause various disorders of the respiratory, nervous and endocrine systems and also cause allergic reactions [1,2]. Of course, the organs of the digestive system are not an exception since exogenous factors are often the provoking factor of intestinal diseases [3].…”