Particular Role of JAK/STAT3 Signaling in Functional Control of Mesenchymal Progenitor Cells

Abstract

The role of JAK/STAT3-mediated signaling pathway in the realization of the growth potential of mesenchymal precursor cells was examined in vitro. The stimulating role of JAKs and STAT3 towards proliferating activity of progenitor cells and their different role in the regulation of differentiation of the progenitor elements were demonstrated. Inhibitors of JAKs and STAT3 reduced the yield of fibroblast CFU and their mitotic activity. Blockade of JAKs accelerated and selective inactivation of STAT3 decelerated differentiation of progenitor cells.

TMEM119 promotes gastric cancer cell migration and invasion through STAT3 signaling pathway

“…STAT signaling pathway plays an important role in many physiological processes such as cell growth and differentiation. 34 In physiological conditions, STAT is temporarily activated. However, the abnormal activation of STAT, especially the continuous activation, is closely related to the occurrence of many tumors.…”

Halogenated (Cl-ion) songorine is a new original agonist of fibroblast growth factor receptors of neuronal-committed progenitors possessing neuroregenerative effect after cerebral ischemia and hypoxia in experimental animals

“…For this PSA-NCAM + cells at a concentration of 105 / ml were incubated for 7 days at 37°С in humidified atmosphere of 95% air and 5% CO2 in «MACS Neuro Medium» (Miltenyi Biotec, Germany). After incubation, we counted content of neuronal-committed progenitors (clonogenic PSA-NCAM + cells) that formed neurospheres containing more than 100 cells (CFU-N) [24][25][26][27].…”

“…Inhibitors of individual signaling molecules and their concentrations were used in the experiment: NF-кB «oridonine» (1 μM); NF-кB, IKK, PKC «aurothiomalate» (50 μM); PI3K «LY294002» (50 μM); ERK1/2 «PD98059» (100 μM); p38 and PKB «SB203580» (10 μM); adenylate cyclase «2',5'dideoxyadenosine» (30 μM); IKK-2 «Inhibitor IV» (2 μM); JNK «SP600125» (10 μM); p53 (5 μM) (Pifithrin-, Cyclic); JAK1.3 «CP 690550» (blocks JAK1 or JAK3, depending on the concentration of 1 nM and 100 nM, respectively); JAK2 was induced with AG 490 (50 μM); STAT3 «STAT3 Inhibitor XIV, LLL12» (5 μM). The working concentrations of the antagonists in vitro were determined in accordance with instructions of the developers of these chemical agents and confirmed as the optimum in own preliminary experiments on the test cultures [14,[27][28][29][30][31]. The specimens of nervous tissue were taken from subventricular area of cerebral hemispheres.…”

Neuroprotective properties of the C-Jun N-terminal kinase (JNK) inhibitor in hypoxic hypoxia

“…Выявленная нами ранее специфика внутриклеточной сигнальной трансдукции в различных типах клеток-предшественников послужила основой для разработки нового запатентованного направления таргетной терапии в регенеративной медицине -«Стратегии фармакологической регуляции внутриклеточной сигнальной трансдукции в регенераторно-компетентных клетках» [7]. Данный подход предполагает использование в качестве «мишеней» фармакологического воздействия отдельных внутриклеточных сигнальных молекул, вовлеченных в процесс реализации ростового потенциала родоначальных ýлементов, либо клеток-регуляторов их функций [7][8][9]. В НИИФиРМ им.…”