Particles in loose hips

Campbell, Paul Douglas; Kossovsky, Nir; Tp, Schmalzried

doi:10.1302/0301-620x.75b1.8421019

Cited by 5 publications

(2 citation statements)

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“…Our research center has been previously instrumental in the development of techniques to isolate and characterize PE particles from metal-on-polyethylene THAs [6,7,35,43]. Expanding on this earlier experience, we have now developed a novel protocol to meet the demands of analyzing newer crosslinked PE particles including enhanced particle separation, purification, and particle display.…”

Section: Introductionmentioning

confidence: 99%

The John Charnley Award: An Accurate and Sensitive Method to Separate, Display, and Characterize Wear Debris: Part 1: Polyethylene Particles

Billi

Benya

Kavanaugh

et al. 2012

Clinical Orthopaedics &Amp; Related Research

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Background Numerous studies indicate highly crosslinked polyethylenes reduce the wear debris volume generated by hip arthroplasty acetabular liners. This, in turns, requires new methods to isolate and characterize them. Questions/purposes We describe a method for extracting polyethylene wear particles from bovine serum typically used in wear tests and for characterizing their size, distribution, and morphology.Methods Serum proteins were completely digested using an optimized enzymatic digestion method that prevented the loss of the smallest particles and minimized their clumping. Density-gradient ultracentrifugation was designed to remove contaminants and recover the particles without filtration, depositing them directly onto a silicon wafer. This provided uniform distribution of the particles and high contrast against the background, facilitating accurate, automated, morphometric image analysis. The accuracy and precision of the new protocol were assessed by recovering and characterizing particles from wear tests of three types of polyethylene acetabular cups (no crosslinking and 5 Mrads and 7.5 Mrads of gamma irradiation crosslinking). Results The new method demonstrated important differences in the particle size distributions and morphologic parameters among the three types of polyethylene that could not be detected using prior isolation methods. Conclusion The new protocol overcomes a number of limitations, such as loss of nanometer-sized particles and artifactual clumping, among others. Clinical Relevance The analysis of polyethylene wear particles produced in joint simulator wear tests of prosthetic joints is a key tool to identify the wear mechanisms that produce the particles and predict and evaluate their effects on periprosthetic tissues.

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Section: Introductionmentioning

confidence: 99%

The John Charnley Award: An Accurate and Sensitive Method to Separate, Display, and Characterize Wear Debris: Part 1: Polyethylene Particles

Billi

Benya

Kavanaugh

et al. 2012

Clinical Orthopaedics &Amp; Related Research

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show abstract

“…Cellular response to the debris is the primary cause that limits the longevity of such implants [1][2][3][4][5][6][7][8][9] . Various cell lineages phagocytose/endocytose such non-biodegradable particulates resulting in the release of chemical entities, which has been hypothesized as one of the fundamental events that occur when wear-debris come in contact with surrounding cells [10][11][12][13][14][15] .…”

Section: Introductionmentioning

confidence: 99%

Phagocytosis of Simulated Nano-wear Debris by Osteoblasts

Musib¹

2012

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In this study I have used well characterized simulated wear-nano-particles of ultra-high molecular weight polyethylene (UHMWPE) of known size and shape to study particulate phagocytosis by MG63 osteoblast-like cells. The particles were treated to decrease their propensity to form aggregates in aqueous suspension and scanning electron microscopy (SEM) was performed to image them as individual particles on 0.01 µm pore size polycarbonate filter membranes. These images were further subjected to morphometric analysis of the particles using ASTM F1877 descriptors [equivalent circle diameter (ECD in μm), aspect ratio (AR), elongation (E), roundness (R), and formfactor (FF)]. The mean (±SD) ECD of the particles were 0.056±0.03 µm. They were subsequently introduced to confluent MG63 cells at particle:cell ratio of 100:1 and incubated for 24 h. Transmission electron micrography (TEM) showed the nanoparticles inside the cytosol. No time dependent response was observed beyond 24 h. The nanoparticles seemed to re-agglomerate once inside the cell. The damage to the cells was evident from the compromised cell membrane. This study will help further our knowledge of the wear-mediated osteolytic process.

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Isolation of predominantly submicron‐sized UHMWPE wear particles from periprosthetic tissues

Campbell

Yeom

et al. 1995

J. Biomed. Mater. Res.

338

190

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A method of tissue digestion using sodium hydroxide was applied to the isolation and recovery of ultra-high-molecular-weight polyethylene (UHMWPE) particles from tissues around failed total hip replacements. Density gradient ultracentrifugation of the digested tissues was performed to separate the UHMWPE from cell debris and other particulates. Fourier transform infrared spectroscopy and differential scanning calorimetry (DSC) verified that the recovered particles were UHMWPE. When viewed by scanning electron microscopy, individual particles were clearly observed and were either rounded or elongated. The majority were submicron in size. The application of this method to the study of particles from periprosthetic tissues may elucidate aspects of biomaterial particle size and shape that are important to the biologic response to, and clinical outcome of, total joint replacement.

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Particles in loose hips

Abstract: We welcome letters to the Editor concerning articles which have recently been published. Such letters will be subject to the usual stages of selection and editing; where appropriate the authors of the original article will be offered the opportunity to reply.

Cited by 5 publications

References 0 publications

The John Charnley Award: An Accurate and Sensitive Method to Separate, Display, and Characterize Wear Debris: Part 1: Polyethylene Particles

The John Charnley Award: An Accurate and Sensitive Method to Separate, Display, and Characterize Wear Debris: Part 1: Polyethylene Particles

Phagocytosis of Simulated Nano-wear Debris by Osteoblasts

Isolation of predominantly submicron‐sized UHMWPE wear particles from periprosthetic tissues

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