Particle separation by dielectrophoresis

Gascoyne, Peter R. C.; Vykoukal, Jody

doi:10.1002/1522-2683(200207)23:13<1973::aid-elps1973>3.0.co;2-1

Cited by 742 publications

(551 citation statements)

References 94 publications

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“…32 In that method, cells reach equilibrium levitation heights above an electrode array and are carried through the separation channel by a hydrodynamic flow profile that causes cell types levitated to different heights to emerge from the channel outlet at different times, thereby effecting fractionation. 10,19,27 During the course of our DEP-FFF studies, the need arose for fractionated cell subpopulations to be collected for pathological examination.…”

Section: Discussionmentioning

confidence: 99%

Dielectrophoretic Segregation of Different Human Cell Types on Microscope Slides

et al. 2005

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A new method for preparing cells for microscopic examination is presented in which cell mixtures are fractionated by dielectrophoretic forces and simultaneously collected into characteristic zones on slides. The method traps cells directly from the suspending medium onto the slide, reducing cell loss. Furthermore, it exploits differences in the dielectric properties of the cells, which sensitively reflect their morphology. Because different cell types are trapped in characteristic zones on the slide, the technique represents an advance over existing methods for slide preparation, such as centrifugation and smears where cells are randomly distributed. In particular, the new method should aid in the detection of rare and anomalous cell subpopulations that might otherwise go unnoticed against a high background of normal cells. As well as being suitable for traditional microscopic examination and automated slide scanning approaches, it is compatible with histochemical and immunochemical techniques, as well as emerging molecular and proteomic methods. This paper describes the rationale and design of this so-called electrosmear instrumentation and shows experimental results that verify the theory and applicability of the method with model cell lines and normal peripheral blood subpopulations. KeywordsSlide preparation; Dielectrophoresis; Cell separation; Cell discrimination; Microelectrodes Advances in cytological slide preparation techniques have facilitated the identification and characterization of different cell types and have improved the ease and accuracy of disease diagnosis. In particular, numerous staining procedures, including chemical dye-, 1 fluorescent-, 2 enzyme-linked-, 3 immunosensitive-, 4 and specific molecular-methods 5 have been developed. While these techniques allow target cells to be differentiated from other cell types, specimen screening efficiency depends on how well the pathologist discriminates target components such as bacteria, precancerous lesions or cancerous cells from background cells. In diseases characterized by the presence of extremely small numbers of marker cells against a background of large numbers of normal cells, identifying the randomly distributed target cells is tedious, time consuming, and prone to error. Recently, a number of instruments have been introduced that automatically scan slides and attempt to identify putative marker cells for later review by a pathologist. 6 These instruments are based on machine vision through a high quality microscope and they are expensive and relatively slow.

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Section: Discussionmentioning

confidence: 99%

Dielectrophoretic Segregation of Different Human Cell Types on Microscope Slides

et al. 2005

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“…DEP refers to the induced motion of particles (either charged or non-charged) in a nonuniform electric field [4]. The electric field gradients in these devices are created by either microelectrodes [5,6] or micro insulators [7][8][9]. In electrode-based dielectrophoresis (eDEP), pairs of microelectrodes are placed inside a microchannel.…”

Section: Introductionmentioning

confidence: 99%

Joule heating effects on electroosmotic flow in insulator‐based dielectrophoresis

et al. 2011

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Joule heating effects on electroosmotic flow in insulator-based dielectrophoresisInsulator-based dielectrophoresis (iDEP) is an emerging technology that has been successfully used to manipulate a variety of particles in microfluidic devices. However, due to the locally amplified electric field around the in-channel insulator, Joule heating often becomes an unavoidable issue that may disturb the electroosmotic flow and affect the particle motion. This work presents the first experimental study of Joule heating effects on electroosmotic flow in a typical iDEP device, e.g. a constriction microchannel, under DC-biased AC voltages. A numerical model is also developed to simulate the observed flow pattern by solving the coupled electric, energy, and fluid equations in a simplified two-dimensional geometry. It is observed that depending on the magnitude of the DC voltage, a pair of counter-rotating fluid circulations can occur at either the downstream end alone or each end of the channel constriction. Moreover, the pair at the downstream end appears larger in size than that at the upstream end due to DC electroosmotic flow. These fluid circulations, which are reasonably simulated by the numerical model, form as a result of the action of the electric field on Joule heatinginduced fluid inhomogeneities in the constriction region.

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“…Since the dielectrophoretic force is proportional to the electric field gradient, it can appear either when direct current (DC) or alternate current (AC) is applied. The non-uniform electric field can be generated using an array of electrodes (electrode based, eDEP) [5][6][7] or an array of insulating posts (insulator based, iDEP).…”

Section: Introductionmentioning

confidence: 99%

A new approach to design an efficient micropost array for enhanced direct-current insulator-based dielectrophoretic trapping

et al. 2016

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Aquesta és una còpia de la versió author's final draft d'un article publicat a la revista Analytical and bioanalytical chemistry.La publicació final està disponible a Springer a través de http://dx.doi.org/10.1007/s00216-016-9629-2 This is a copy of the author 's final draft version of an article published in the journal Analytical and bioanalytical chemistry. AbstractDirect-current insulator-based dielectrophoresis (DC-iDEP) is a well-known technique that benefits from the electric field gradients generated by an array of insulating posts to separate or trap biological particles. In this work, we propose a novel figure of merit to find an efficient design of the post-array distribution in a microfluidic channel. To characterization can be used to reduce the required electric field to achieve effective particle trapping and, therefore, avoid the negative effects of Joule heating in cells or viable particles.

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Particle separation by dielectrophoresis

Abstract: The application of dielectrophoresis to particle discrimination, separation, and fractionation is reviewed, some advantages and disadvantages of currently available approaches are considered, and some caveats are noted.

Cited by 742 publications

References 94 publications

Dielectrophoretic Segregation of Different Human Cell Types on Microscope Slides

Dielectrophoretic Segregation of Different Human Cell Types on Microscope Slides

Joule heating effects on electroosmotic flow in insulator‐based dielectrophoresis

A new approach to design an efficient micropost array for enhanced direct-current insulator-based dielectrophoretic trapping

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