Particle/cell separation on microfluidic platforms based on centrifugation effect: a review

Al-Faqheri, Wisam; Thio, Tzer Hwai Gilbert; Qasaimeh, Mohammad A.; Dietzel, Andreas; Madou, Marc; Al-Halhouli, Ala’aldeen

doi:10.1007/s10404-017-1933-4

Cited by 96 publications

(77 citation statements)

References 79 publications

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“…. A variety of force fields, ranging from the ubiquitous gravity to centrifugal , acoustic , electric , magnetic , optical , and flow fields, have thus far been demonstrated to manipulate particles for microfluidic applications. Each of these fields generates a particle motion, whose dependence on particle size and other physicochemical properties is significantly different from each other (Table ).…”

Section: Introductionmentioning

confidence: 99%

“…DC electric fields are involved in capillary (zone) electrophoresis [32], which drive both fluid electroosmosis and particle electrophoresis through straight uniform (both geometrically and physicochemically) microchannels [33,34]. A hydrodynamic pumping of the particulate solution, which is required in nearly all other field Gravity b) Sedimentation and centrifugation: Relative density of particles and fluids [11] a 2 Acoustic Acoustophoresis: Relative density and compressibility of particles and fluids [12] a 2…”

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confidence: 99%

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Recent advances in direct current electrokinetic manipulation of particles for microfluidic applications

Xuan

2019

Electrophoresis

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Microfluidic devices have been extensively used to achieve precise transport and placement of a variety of particles for numerous applications. A range of force fields have thus far been demonstrated to control the motion of particles in microchannels. Among them, electric field‐driven particle manipulation may be the most popular and versatile technique because of its general applicability and adaptability as well as the ease of operation and integration into lab‐on‐a‐chip systems. This article is aimed to review the recent advances in direct current (DC) (and as well DC‐biased alternating current) electrokinetic manipulation of particles for microfluidic applications. The electric voltages are applied through electrodes that are positioned into the distant channel‐end reservoirs for a concurrent transport of the suspending fluid and manipulation of the suspended particles. The focus of this review is upon the cross‐stream nonlinear electrokinetic motions of particles in the linear electroosmotic flow of fluids, which enable the diverse control of particle transport in microchannels via the wall‐induced electrical lift and/or the insulating structure‐induced dielectrophoretic force.

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Section: Introductionmentioning

confidence: 99%

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confidence: 99%

Recent advances in direct current electrokinetic manipulation of particles for microfluidic applications

Xuan

2019

Electrophoresis

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“…A comprehensive review of centrifugation forces for cell separation can be found in ref. . Hydrodynamic filtration is another size‐based separation technique in microfluidics .…”

Section: Microfluidic Systems For Single‐cell Manipulationmentioning

confidence: 99%

Recent Advances and Perspectives in Microfluidics‐Based Single‐Cell Biosensing Techniques

Brimmo

Qasaimeh

et al. 2017

Small Methods

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Single‐cell analyses of secretory proteins are essential to fully understand cellular functional heterogeneity and unravel the underlying mechanisms of intercellular signaling and interactions. Retrieving dynamic information of protein secretion at single‐cell resolution reflects the precise, real‐time functional states of individual cells in physiological processes. Such measurements remain very challenging in single‐cell analysis, which requires highly integrated systems capable of performing on‐chip single‐cell isolation and subsequent real‐time protein detection. Here, recent advances in microfluidics‐based single‐cell manipulation and emerging approaches for label‐free single‐cell biosensing are reviewed. The advantages and limitations of these technologies are summarized and challenges to establish the integrated microfluidic biosensing systems for real‐time single‐cell secretomics are discussed. Recent efforts on integrated platforms for on‐chip single‐cell protein assays are highlighted and some perspectives on future directions in this field are provided.

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“…The flow rate and direction can be controlled by controlling the spinning speed of the microfluidic CD. By implementing this pumping method, only a compact and accurately-controlled motor is required, without the need for external syringe pumps [13]. This can also eliminate environmental contamination and minimize air bubbles that are usually introduced with the utilization of external pumping mechanisms.…”

Section: Introductionmentioning

confidence: 99%

Development of Active Centrifugal Pump for Microfluidic CD Platforms

et al. 2020

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The continuous emerging of microfluidic compact disc (CD) platforms for various real-life applications motivates researchers to explore new innovative ideas towards more integrated active functions. However, microfluidic CDs have some drawbacks, including the unidirectional flow that limits the usable space for multi-stepped biological and chemical assays. In this work, a novel active and bidirectional centrifugal pump is developed and integrated on microfluidic CDs. The design of the developed pump partially replicates the designs of the conventional centrifugal pumps with a modification in the connecting channels’ positions that allow the developed pump to be reversible. The main advantage of the proposed centrifugal pump is that the pumping speed can be accurately controlled during spinning or while the microfluidic CD is stationary. Performance tests show that the pumping speed can reach up to 164.93 mm3/s at a pump rotational speed (impellers speed) of 4288 rpm. At that speed, 1 mL of water could be pumped in 6.06 s. To present a few of the potential applications of the centrifugal pump, flow reciprocation, bidirectional pumping, and flow switching were performed and evaluated. Results show that the developed centrifugal pump can pump 1096 µL of liquid towards the CD center at 87% pumping efficiency while spinning the microfluidic CD at 250 rpm. This novel centrifugal pump can significantly widen the range of the applicability of microfluidic CDs in advanced chemical processes and biological assays.

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Particle/cell separation on microfluidic platforms based on centrifugation effect: a review

Cited by 96 publications

References 79 publications

Recent advances in direct current electrokinetic manipulation of particles for microfluidic applications

Recent advances in direct current electrokinetic manipulation of particles for microfluidic applications

Recent Advances and Perspectives in Microfluidics‐Based Single‐Cell Biosensing Techniques

Development of Active Centrifugal Pump for Microfluidic CD Platforms

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