Participation of Plant Hormones in Determination and Progression of Somatic Embryogenesis

García, Víctor Jiménez; Thomas, Clément

doi:10.1007/7089_034

Cited by 38 publications

(28 citation statements)

References 75 publications

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“…In other systems, ethylene has been shown to have a role in cotyledon development of B. napus microspore embryos (Hays et al, 2000) and in the development and conversion of alfalfa somatic embryos (Kepczynska et al, 2009;Kepczynska and Zielinska, 2011). In some situations, a suppression of embryogenesis with ethylene is observed (for review, see Jiménez and Thomas, 2006), and a number of systems show enhanced SE by the addition of AgNO 3 to medium (for review, see Kumar et al, 2009). Thus, ethylene effects on SE vary with species and culture system.…”

Section: Stress Ethylene and Somatic Embryogenesismentioning

confidence: 99%

AGAMOUS-Like15 Promotes Somatic Embryogenesis in Arabidopsis and Soybean in Part by the Control of Ethylene Biosynthesis and Response

2013

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Many of the regulatory processes occurring during plant embryogenesis are still unknown. Relatively few cells are involved, and they are embedded within maternal tissues, making this developmental phase difficult to study. Somatic embryogenesis is a more accessible system, and many important regulatory genes appear to function similar to zygotic development, making somatic embryogenesis a valuable model for the study of zygotic processes. To better understand the role of the Arabidopsis (Arabidopsis thaliana) MADS factor AGAMOUS-Like15 (AGL15) in the promotion of somatic embryogenesis, direct target genes were identified by chromatin immunoprecipitation-tiling arrays and expression arrays. One potential directly up-regulated target was At5g61590, which encodes a member of the ethylene response factor subfamily B-3 of APETALA2/ETHYLENE RESPONSE FACTOR transcription factors and is related to Medicago truncatula SOMATIC EMBRYO-RELATED FACTOR1 (MtSERF1), which has been shown to be required for somatic embryogenesis in M. truncatula. Here, we report confirmation that At5g61590 is a directly expressed target of AGL15 and that At5g61590 is essential for AGL15's promotion of somatic embryogenesis. Because At5g61590 is a member of the ETHYLENE RESPONSE FACTOR family, effects of ethylene on somatic embryogenesis were investigated. Precursors to ethylene stimulate somatic embryogenesis, whereas inhibitors of ethylene synthesis or perception reduce somatic embryogenesis. To extend findings to a crop plant, we investigated the effects of ethylene on somatic embryogenesis in soybean (Glycine max). Furthermore, we found that a potential ortholog of AGL15 in soybean (GmAGL15) upregulates ethylene biosynthesis and response, including direct regulation of soybean orthologs of At5g61590/MtSERF1 named here GmSERF1 and GmSERF2, in concordance with the M. truncatula nomenclature.

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Section: Stress Ethylene and Somatic Embryogenesismentioning

confidence: 99%

AGAMOUS-Like15 Promotes Somatic Embryogenesis in Arabidopsis and Soybean in Part by the Control of Ethylene Biosynthesis and Response

2013

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“…Also, it is important to mention that the hormonal endogenous substances play important roles in the somatic embryogenic process. Out of the above mentioned GRS, auxins are the most important components in the induction of the process (Dodeman et al, 1997;Jiménez, 2005;Jiménez & Thomas, 2006;Rao, 1996;Feher, 2006). Somatic cells need the signal for the cell polarization and the asymmetric division given by auxins as it happens in their zygotic counterparts (Gutiérrez-Mora et al, 2004;Pagnussat et al, 2009).…”

Section: Somatic Embryogenesismentioning

confidence: 99%

Plant Somatic Embryogenesis: Some Useful Considerations

Gutiérrez-Mora¹,

González-Gutiérrez²,

Rodrı́guez-Garay³

et al. 2012

Embryogenesis

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“…In particular, enhanced synthesis of galanthamine accompanied development of somatic embryos [17]. Somatic embryogenesis may be influenced by many factors [18], including ethylene production [12] and growth regulators like auxins. Embryogenic callus is usually formed in auxin-supplemented media, and the most commonly used auxin for this purpose is 2,4-D [19].…”

Section: Introductionmentioning

confidence: 99%

Influence of auxins on somatic embryogenesis and alkaloid accumulation in Leucojum aestivum callus

et al. 2013

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In vitro cultures of Leucojum aestivum are considered as an alternative for the production of galanthamine, which is used for the symptomatic treatment of Alzheimer’s disease. We studied the effects of auxins 2,4-dichlorophenoxyacetic acid (2,4-D), 4-amino-3,5,6-trichloropicolinic acid (picloram), 3,6-dichloro-o-anisic acid (dicamba) at concentrations of 25 and 50 µM on the induction of embryogenic callus and its capacity to induce somatic embryogenesis and alkaloid accumulation. The embryogenic response of the explants was from 30% for 25 µM of dicamba to 100% for picloram (for both 25 and 50 µM). 2,4-D (50 µM) stimulated greater callus proliferation and somatic embryo induction as compared to the other auxins. Polyethylene glycol (PEG) stimulated somatic embryo maturation. Callus grown on media containing 50 µM of auxins produced fewer phenolic compounds as compared with callus grown on media containing 25 µM of auxins. GC-MS analyses showed seven alkaloids in the in vivo bulbs and two to four in callus culture. Galanthamine was detected in callus cultivated with 2,4-D (25, 50 µM), picloram (25 µM), and dicamba (50 µM). Other alkaloids, trisphaeridine, tazettine, and 11-hydroxyvittatine were accumulated only in callus growing on medium with picloram (50 µM).

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Participation of Plant Hormones in Determination and Progression of Somatic Embryogenesis

Cited by 38 publications

References 75 publications

AGAMOUS-Like15 Promotes Somatic Embryogenesis in Arabidopsis and Soybean in Part by the Control of Ethylene Biosynthesis and Response

AGAMOUS-Like15 Promotes Somatic Embryogenesis in Arabidopsis and Soybean in Part by the Control of Ethylene Biosynthesis and Response

Plant Somatic Embryogenesis: Some Useful Considerations

Influence of auxins on somatic embryogenesis and alkaloid accumulation in Leucojum aestivum callus

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