Participation of mesenchymal precursor cells in wound healing on skin flap model

Abstract

We studied the state of different pools of mesenchymal precursor cells in the bone marrow, peripheral blood, and wound surface after modeling of tissue damage by skin flap removal. The participation of regional and circulating stromal precursors in the healing of skin defect and the absence of compensatory reaction of the regeneratory process deep reserve, mesenchymal stem cells of the bone marrow, was demonstrated.

Dopamine Mobilizes Mesenchymal Progenitor Cells Through D2-Class Receptors and Their PI3K/AKT Pathway

“…Hematopoietic and endothelial progenitors increase transitorily their numbers in PB after a traumatic event. In addition, recent reports suggested that mesenchymal cells would be mobilized in humans after injuries such as wound healing , hip fracture , or cardiac and/or respiratory failure . In this regard, we previously described a similar result in acute skeletal muscle injury in subjects running a long distance race .…”

Skin regeneration with bone marrow-derived cell populations

“…Wound healing is still an actual problem of regenerative medicine. The investigation of the processes occurring during the regeneration of skin tissue will open new possibilities for cell therapy [14]. The objective of our study was the investigation of the effect of bone marrow-derived stem cells for the regeneration of skin tissue in BALB/c mouse in a full-thickness wound model.…”

Wound healing properties of the protein kinase A inhibitor and the mechanisms of their development

“…Step 8: The study of mesenchymal progenitors content in the wound, their functional activity, and production of growth factors by stromal cells from the surface of the wound. Goldberg et al, 2006;Zyuz'kov et al, 2012 Reference (video) Öz et al, 2016;Qureshi et al, 2019 Liability Company Assortiment Firm, Sergiev Posad city, Russia), water ad libitum. To exclude seasonal fluctuations of studied parameters, all the experiments were performed in the autumn-winter period.…”

“…The pool of CFU in the S-phase of the cell cycle was determined according to the formula: N = [(a-b)/a] × 100%, where a is the average for the group the number of CFU-F from cells not treated with hydroxyurea; b -the average for the group the number of CFU-F from cells treated with hydroxyurea. The intensity of the processes of specialization of progenitors was determined by calculating the ratio of the corresponding clusterforming (ClFU-F, clusters containing 20-30 cells) to CFU -F (differentiation index) (Goldberg et al, 2006, Zyuz'kov et al, 2012.…”

“…These products should not only speed up the process of tissue repair but also lead to the formation of full-fledged skin (Dehkordi et al, 2019). At the same time, it is believed that achieving such a result is possible due to the pharmacological activation of resident progenitor cells functions of the skin and the underlying tissues (Driskell et al, 2013;Motegi et al, 2017), as well as the mobilization and migration of multipotent stem cells (SC) from their tissue-depots (primarily from bone marrow) (Goldberg et al, 2006;Mishra et al, 2017;Fu et al, 2019).…”