Partially Fertile Line with Apospory Obtained from Tissue Culture of Male Sterile Plant of Sorghum (Sorghum bicolor L. Moench)

Elkonin, L. A.; Enaleeva, N. Kh.; Tsvetova, M. I.; Belyaeva, E. V.; Ishin, A. G.

doi:10.1006/anbo.1995.1108

Cited by 10 publications

(6 citation statements)

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“…It also suggests that the gene(s) controlling apomixis have diploid origin(s), with apospory mapping to a distinct subgenome in buffelgrass. Even though expression is quite low, reports of apomixis in diploid Sorghum bicolor (L.) Moench (Rao and Narayana, 1968; Elkonin et al, 1995) and Brachiaria decumbens (Stapf) R. D. Webster (Naumova et al, 1999) also suggest the presence of apomixis gene(s) in diploid genomes. These findings indicate potential to clone the apomixis gene(s) from bacterial artificial chromosome (BAC) libraries of the relatively small genomes in these species.…”

Section: Discussionmentioning

confidence: 99%

Disomic Inheritance, Suppressed Recombination, and Allelic Interactions Govern Apospory in Buffelgrass as Revealed by Genome Mapping

et al. 2002

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lecular markers within mapping populations (Wendel and Parks, 1984;Torres et al., 1985; Molecular tools have not identified the gene(s) governing apomixis Paterson et al., 1988Paterson et al., , 1991Saito et al., 1991; Lyt-nor have they been used to successfully transfer the trait to important, sexually reproducing food crops. Several molecular studies addressing tle, 1991;Schon et al., 1991; Zivy et al., 1992; Causse et apomixis in grasses have used interspecific and intergeneric hybrids. al., 1994; Chittenden et al., 1994). This loss of specific The failure to recover specific F 1 genotypes from these wide crosses genotypes from the progeny may be due to interactions can be caused by unfavorable interactions between the gametes, zybetween genes of the two species within gametes, zygote, embryo, endosperm, and/ or maternal tissue. These interactions gote, embryo, endosperm, or maternal tissue (Hadley can eliminate recombinant genotypes with valuable information toand Openshaw, 1980). Differential viability of offspring wards linkage analyses of the trait. Buffelgrass [Pennisetum ciliare because of allelic interactions has been reported in (L.) Link syn. Cenchrus ciliaris L.], a polymorphic species with intermaize (Zea mays L.)-Tripsacum hybrids (Maguire, fertile apomictic and sexual genotypes, offers an opportunity to genetically map apomixis by means of intraspecific hybrids with euploid 1963), tomato, Lycopersicon esculentum Mill. (Rick, genomes. This study reports a linkage map of the apospory region in 1966), wheat, Triticum aestivum L. (Manabe et al., 1999), buffelgrass. Apospory, classified by progeny testing and cytologically and rice,

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Section: Discussionmentioning

confidence: 99%

Disomic Inheritance, Suppressed Recombination, and Allelic Interactions Govern Apospory in Buffelgrass as Revealed by Genome Mapping

et al. 2002

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“…The line originates from a regenerant that was grown from a callus obtained from a plant with cytoplasmic male sterility (CMS) and was characterized by a rever sion to partial male fertility [19]. Plants were grown on experimental plots of the Plant Breeding Center of (Agricultural Research Institute for the Southeastern Region, Saratov) from 2000 to 2009.…”

Section: Methodsmentioning

confidence: 99%

“…We have previously identified a grain sorghum line with elements of apomixis [19]. Cytoembryological analysis was performed for the male sterile plants of the line that set seeds on panicles preserving "fresh" stigmata for 10-12 days after starting flowering, and the plants were found to have aposporous ESs (apo ESs), initial cells of apo ESs, and autonomously developing proembryos.…”

Section: Introductionmentioning

confidence: 99%

Expression of the apomictic potential and selection for apomixis in sorghum line AS-1a

2012

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Expression of elements of apomixis was studied for ten seasons in sorghum line AS 1a and its backcross hybrids in the 9E and A3 sterile cytoplasms. Cytoembryological analysis revealed aposporous embryo sacks (apo ESs), their initial cells, and, rare, parthenogeneic proembryos in ovules of line AS 1a and its BC 2 and BC 3 hybrids on the 9E cytoplasm. The A3 sterile cytoplasm suppressed the development of par thenogenetic proembryos, but did not affect the apo ES formation. The frequency of apomictic elements increased in seasons with high daily temperatures and total precipitation deficiency in the period of ovule and megagametophyte development (r = -0.805, p < .01). Selection was used to isolate the families where the fre quency of ovules with apo ESs was 28% and the frequency of parthenogenetic proembryos was 14%. Emas culated panicles of line AS 1a were pollinated with pollen of line Volzhskoe 4v, which carried the Rs marker dominant gene, responsible for the anthocyan color of coleoptyles and leaves in seedlings. Plants of the maternal type were found in the progenies of these crosses at a frequency of 1.4-28.1%. The genetic structure of the endosperm in grains with maternal type seedlings was inferred from the electrophoretic patterns of storage proteins (kafirins). The kafirin spectra of grains producing maternal type seedlings was similar to the spectrum of line AS 1a and differed from the spectra of grains producing hybrid seedlings, indicating that the endosperm developed autonomously when apomictic grains formed in line AS 1a. The results showed that lines with facultative apomixis can be constructed in functionally diploid plants.

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“…It was found that young anthers produced more callus as compared to mature anthers. A number of reports are available on studies concerning haploid sorghum culture and culture of male sterile lines (Rose et al, 1986a, b;Elkonin et al, 1987Elkonin et al, , 1993Elkonin et al, , 1994Elkonin et al, , 1995Elkonin, 1995;Elkonin andEnaleeva, 1997, 1998). Cytological analysis of meiosis in sterile plants by Elkonin and Enaleeva (1998) revealed that the tissue culture-induced mutation caused significant abnormalities in chromosome synapsis during prophase-I that resulted in the formation of multiple micronuclei during cell division.…”

Section: In Vitro Plant Regeneration Haploidy Polyploidy and Somaclmentioning

confidence: 96%

Biotechnology: Genetic improvement of sorghum (Sorghum bicolor (L.) Moench)

Maqbool

Devi

Sticklen

2001

In Vitro Cell.Dev.Biol.-Plant

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This report reviews the contributions to the improvement of sorghum (Sorghum bicolor (L.) Moench) through traditional approaches with emphasis on the application of biotechnological methods. Strategies include breeding for higher yield, improved grain quality, and biotic and abiotic stress tolerance. Hybrid development and polyploidy breeding are also discussed. Plant breeders, working in concert with biotechnologists, have developed new powerful tools for plant genetic manipulation and genotype evaluation that will significantly improve the efficiency of plant breeding. Improving sorghum through biotechnology is the latest in a long series of technologies that have been applied to this crop. Five basic tools of technology have been developed for sorghum improvement: (1) in vitro protocols for efficient plant regeneration; (2) molecular markers; (3) gene identification and cloning; (4) genetic engineering and gene transfer technology to integrate desirable traits into the sorghum genome; and (5) genomics and germplasm databases. Reports on studies involving the problems, progress, and prospects for utilizing the biotechnological methods for sorghum improvement are discussed.

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Partially Fertile Line with Apospory Obtained from Tissue Culture of Male Sterile Plant of Sorghum (Sorghum bicolor L. Moench)

Cited by 10 publications

References 0 publications

Disomic Inheritance, Suppressed Recombination, and Allelic Interactions Govern Apospory in Buffelgrass as Revealed by Genome Mapping

Disomic Inheritance, Suppressed Recombination, and Allelic Interactions Govern Apospory in Buffelgrass as Revealed by Genome Mapping

Expression of the apomictic potential and selection for apomixis in sorghum line AS-1a

Biotechnology: Genetic improvement of sorghum (Sorghum bicolor (L.) Moench)

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