Partial replacement of fishmeal by fermented soybean meal in diets for juvenile white shrimp (Litopenaeus vannamei)

Abstract

As an important feed ingredient, fermented soybean meal (FSM) has been widely used in aquatic animals due to its stable sources and reasonable price. Here, we evaluated the potential for replacement of fishmeal (FM) with FSM in diet of Litopenaeus vannamei. Five isonitrogenous (410 g/kg) and isolipidic (80 g/kg) diets were formulated: a control diet containing 320 g/kg FM and four experimental diets in which FM in control diet was replaced by FSM at 10 (FSM10%), 20 (FSM20%), 30 (FSM30%) and 40% (FSM40%). An 8-week feeding trial was conducted in fifteen fibreglass tanks with 50 shrimps per tank. After 8 weeks trial, FSM20% had significantly enhanced growth performance (p < 0.05). No significant difference was found in body composition and digestive enzyme activities of all groups (p > 0.05). Through real-time quantitative PCR analysis, tor and s6k expression levels of FSM20% were significantly up-regulated (p < 0.05). Results of western blot showed that the phosphorylation of S6K was not significantly affected by different dietary treatments (p > 0.05), which suggested mTOR signalling pathway was not affected by FSM diets. Based on the above data, 20% replacement of FM with FSM was reasonable and advantageous for L. vannamei diet. K E Y W O R D Sdigestive enzymes, fermented soybean meal, fishmeal, growth performance, Litopenaeus vannamei, mTOR signalling pathway

Effects of partial replacement of dietary fish meal by bioprocessed plant protein concentrates on growth performance, hematology, nutrient digestibility and digestive enzyme activities in juvenile Pacific white shrimp, Litopenaeus vannamei

“…Qiu and Davis reported that plant‐based spray‐dried fermented biomass can replace up to 50% of FM, which is in line with the results of the present study. In this study, FE and PER showed the same trends as WG and SGR which is in agreement with the results of Shao et al . Survivability was not affected by the dietary treatments indicating that whiteleg shrimps have resistance to increasing BPC levels in the diets which is line with the findings of Qiu and Davis .…”

“…reported that 50% FM can be replaced in the diet of white shrimp using rice protein concentrate. Recently, Shao et al . found that 20% of FM could be successfully replaced by fermented SBM in the diet of white shrimp which is lower than that of the present study.…”

“…Kokou et al ., Azarm and Lee, Kader et al . and Shao et al . reported that bioprocessing or fermentation of SBM can increase the FM replacement level in fish and shrimp.…”

“…There have been many works executed concerning soy protein or CGM in order to replace FM in the diet of Pacific white shrimp . However, few works have been done on fermented SBM and CGM as substitutes of FM . Researchers are now putting emphasis on producing high‐quality plant‐based protein through solid‐state fermentation which can be cost‐effective, free of ANFs as well as digestible to increase the production of healthy shrimp.…”

Feeding diets with graded levels of fermented soybean meal to Pacific whiteleg shrimp Penaeus vannamei (Boone, 1931): Effect on digestive enzymes, immune responses and carcass composition

“…The scenario of diminished availability and escalating price of fishmeal in recent years prompted the researchers to substitute its considerable quantity by using various alternatives. One among them is fermented oilseed meals/cakes (Shiu et al, 2015;Sun et al, 2015;Sharawy et al, 2016;Jannathulla et al, , 2018a. Though fermented plant proteins are being used as a potential substitute of fishmeal in shrimp feed, its relationship on digestive enzymes and their distribution pattern in P. vannamei is scarce.…”