Partial purification and some biochemical properties of acid phosphatase in germinating chickpea (<i>Cicer arietinum</i>) seeds

Angosto, Trinidad; González, F.; Matilla, Ángel J.

doi:10.1111/j.1399-3054.1988.tb02042.x

Cited by 12 publications

(7 citation statements)

References 25 publications

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“…5). The optimum pH of the Apases purified from plant tissues differed depending on the plant species and tissues, with value ranging from pH 4 to pH 6 (Hasegawa et al 1976;Mclachlan 1980;Dracup et al 1984;Angosto et al 1988;Tanaka et al 1990). The optimum pH of Apase-T also appeared to be in this range and was very similar to the optimum pH of Apase purified from cultured tobacco cells (Ninomiya et al 1977).…”

Section: 5mentioning

confidence: 99%

“…Apase-L showed a higher relative activity for UTP, CTP, and lysolecithin than Apase-T, whereas, Apase-T showed a higher relative activity for D-myo-inositol-l,4,5-tris-phosphate than Apase-L. Glycerolphosphate, glucose and fructose phosphate, NADP, and inositol-l,3,4,5-tetraphosphate were not hydrolyzed by both Apases. It has been documented that the Apases purified from plant tissues display a broad substrate specificity, which enabled to hydrolyze nucleotides, phospholipids and sugar phosphates, but not phytin (Shaw 1966;Ching et al 1987;Dua and Amma 1987;Angosto et al 1988;Kaneko et al 1990). The secretory Apase also showed a similar broad substrate specificity.…”

Section: 5mentioning

confidence: 99%

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Comparison of Characteristics of Acid Phosphatases Secreted from Roots of Lupin and Tomato

Li¹,

Tadano²

1996

Soil Science and Plant Nutrition

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Section: 5mentioning

confidence: 99%

Section: 5mentioning

confidence: 99%

Comparison of Characteristics of Acid Phosphatases Secreted from Roots of Lupin and Tomato

Li¹,

Tadano²

1996

Soil Science and Plant Nutrition

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“…The optimum pH was 4.3, which is ecologically significant because it indicates that the enzyme performs well in low pH soils where the availability of phosphate is mostly limited. As the optimum pH of acid phosphatase of the plants reported ranged from 4.5 to 6.0 (Basha 1984;Ching et al 1987;Angosto et al 1988;Kaneko et al 1990;Ullah and Gibson 1988;Tanaka et al 1990;Yoshimoto et al 1992), secreted acid phosphatase from lupin roots appeared to be adapted to much lower pH conditions.…”

Section: H a R A C T E R I S T I C S Of S E C R E T E D A C I D P Hmentioning

confidence: 99%

Purification and properties of acid phosphatase secreted from lupin roots under phosphorus-deficiency conditions

Ozawa

Osaki

Matsui

et al. 1995

Soil Science and Plant Nutrition

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“…2A, inset). This suggests that the axis exercises some sort of control over ACC-synthase production, just as it does in other metabolic activities (2,21). Supraoptimal temperatures appear to stimulate ACC-synthase activity (Fig.…”

Section: Discussionmentioning

confidence: 99%

Ethylene Production and 1-Aminocyclopropane-1-Carboxylic Acid Conjugation in Thermoinhibited Cicer arietinum L. Seeds

Gallardo¹,

Delgado²,

Sánchez‐Calle³

et al. 1991

Plant Physiol.

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The effect of supraoptimal temperatures (300C, 350C) on germination and ethylene production of Cicer arietinum (chick-pea) seeds was measured. Compared with a 250C control, these temperatures inhibited both germination and ethylene production. The effect of supraoptimal temperatures could be alleviated by treating the seeds with ethylene. It was concluded that one effect of high temperature on germination was due to its negative effect on ethylene production. This inhibitory effect of high temperature was due to increased conjugation of 1-aminocyclopropane-1-carboxylic acid to 1-(malonylamino)cyclopropane-1-carboxylic acid and to an inhibition of ethylene-forming enzyme activity.Seeds germinate at a certain optimum temperature, above which germination may be delayed or inhibited, a phenomenon known as thermoinhibition (1). This can be overcome by various phytohormones, one of which is ethylene, and indeed small quantities of this phytohormone are produced in some thermoinhibited seeds (1, 18).The optimum temperature for the germination of Cicer arietinum seeds is 25°C. At 30°C and above the emergence of the radicle is delayed and the capacity for ion exchange in the embryonic axis (1 1), genetic expression (26), and cytokinin levels (25) are all affected. At 25°C ethylene production is related to cell growth (28). In other tissues high temperatures appear to inhibit the conversion of ACC2 into ethylene rather than interfere with the SAM to ACC step (8).In plants ethylene is synthesized from methionine via adenosyl-methionine and ACC (30). The conversion of SAM or adenosyl-methionine into ACC is catalyzed in the cytosol by ACC-synthase and is the principal point ofcontrol for biosynthesis ofethylene (29). The mechanism for the transformation of ACC into ethylene is less well understood. However, EFE activity has been associated with the vacuole membranes (10) and other compartments of the cell (15).ACC is also metabolized into MACC in the cytosol (4 (14), in some tissues the possibility exists that MACC is converted into ACC via MACC-acylase. MATERIALS AND METHODS Vegetable Materials and Seed GerminationSeeds of Cicer arietinum cv Castellana (chick-pea) were bought commercially and stored at 4°C until required.The seeds were washed in sterile, double-distilled water and incubated in batches of 50 at 25°C, 30°C or 35C, for a minimum of 6 h and a maximum of 36 h, in plastic trays containing 175 mL sterile distilled water. The percentage of germination was measured for all the times and temperatures, taking the emergence of the radicle as the definition of germination.The embryonic axes and cotyledons were aseptically removed after incubation to measure ethylene production, levels of free and conjugated ACC, and activity of ACC-synthase and EFE. Ethylene MeasurementsTwenty five (500 mg) isolated embryonic axes and 10 (4.5 g) cotyledons were aseptically transferred to 50 mL flasks containing 0.5 mL distilled water. The flasks were sealed with silicone-rubber stoppers and incubated in darkness at 25°C. After ...

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Partial purification and some biochemical properties of acid phosphatase in germinating chickpea (Cicer arietinum) seeds

Cited by 12 publications

References 25 publications

Comparison of Characteristics of Acid Phosphatases Secreted from Roots of Lupin and Tomato

Comparison of Characteristics of Acid Phosphatases Secreted from Roots of Lupin and Tomato

Purification and properties of acid phosphatase secreted from lupin roots under phosphorus-deficiency conditions

Ethylene Production and 1-Aminocyclopropane-1-Carboxylic Acid Conjugation in Thermoinhibited Cicer arietinum L. Seeds

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