Comparison of Characteristics of Acid Phosphatases Secreted from Roots of Lupin and Tomato

Li, Minggang; Tadano, Toshiaki

doi:10.1080/00380768.1996.10416623

Cited by 32 publications

(23 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This is consistent with the observation of Basha (1983), who purified a 240-kD APase from peanut seeds that was found to be composed of six identical 42.5-kD subunits. APases secreted by tomato and lupine roots were purified and characterized by Li and Tadano (1996) and were found to be of 68 and 72 kD, respectively. Both of these secreted enzymes were homodimers consisting of two identical subunits.…”

Section: Discussionmentioning

confidence: 99%

“…Plants respond to low levels of bioavailable P by increased root growth, higher expression of P-transporters (Muchhal et al, 1996;Leggewie et al, 1997;Liu et al, 1998;Burleigh and Harrison, 1999), and by alterations in metabolism including the induction of RNAses (Beriola et al, 1994). In addition, secretion of acid phosphatases from roots (APases; EC 3.1.3.2) is a notable consequence of P deficiency (Goldstein et al, 1988;Duff et al, 1991;Li and Tadano, 1996). The levels of induction of APase production and secretion in roots can be dramatic.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Characterization of Arabidopsis Acid Phosphatase Promoter and Regulation of Acid Phosphatase Expression

et al. 2000

View full text Add to dashboard Cite

The expression and secretion of acid phosphatase (APase) was investigated in Indian mustard (Brassica juncea L. Czern.) plants using sensitive in vitro and activity gel assays. Phosphorus (P) starvation induced two APases in Indian mustard roots, only one of which was secreted. Northern-blot analysis indicated transcriptional regulation of APase expression. Polymerase chain reaction and Southern-blot analyses revealed two APase homologs in Indian mustard, whereas in Arabidopsis, only one APase homolog was detected. The Arabidopsis APase promoter region was cloned and fused to the ␤-glucuronidase (GUS) and green fluorescent protein (GFP) reporter genes. GUS expression was first evident in leaves of the P-starved Arabidopsis plants. In P-starved roots, the expression of GUS initiated in lateral root meristems followed by generalized expression throughout the root. GUS expression diminished with the addition of P to the medium. Expression of GFP in P-starved roots also initiated in the lateral root meristems and the recombinant GFP with the APase signal peptide was secreted by the roots into the medium. The APase promoter was specifically activated by low P levels. The removal of other essential elements or the addition of salicylic or jasmonic acids, known inducers of gene expression, did not activate the APase promoter. This novel APase promoter may be used as a plant-inducible gene expression system for the production of recombinant proteins and as a tool to study P metabolism in plants.

show abstract

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

Characterization of Arabidopsis Acid Phosphatase Promoter and Regulation of Acid Phosphatase Expression

et al. 2000

View full text Add to dashboard Cite

show abstract

“…Several PSI-secreted APases have been molecularly characterized in higher plants, including those in white lupin (Lupinus albus; Ozawa et al, 1995;Li and Tadano, 1996;Miller et al, 2001), tomato (Solanum lycopersicum; Bozzo et al, 2002Bozzo et al, , 2006, common bean (Phaseolus vulgaris; Liang et al, 2010), tobacco (Nicotiana tabacum; Lung et al, 2008), and Arabidopsis (Tran et al, 2010b). Some of these secreted enzymes are PAPs, which are purple in water solution because of a Tyr-to-Fe(III) charge transfer transition (Olczak et al, 2003).…”

mentioning

confidence: 99%

The Arabidopsis Purple Acid Phosphatase AtPAP10 Is Predominantly Associated with the Root Surface and Plays an Important Role in Plant Tolerance to Phosphate Limitation

et al. 2011

View full text Add to dashboard Cite

Induction of secreted acid phosphatase (APase) is a universal response of higher plants to phosphate (Pi) limitation. These enzymes are thought to scavenge Pi from organophosphate compounds in the rhizosphere and thus to increase Pi availability to plants when Pi is deficient. The tight association of secreted APase with the root surface may make plants more efficient in the utilization of soil Pi around root tissues, which is present in organophosphate forms. To date, however, no systematic molecular, biochemical, and functional studies have been reported for any of the Pi starvation-induced APases that are associated with the root surface after secretion. In this work, using genetic and molecular approaches, we identified Arabidopsis (Arabidopsis thaliana) Purple Acid Phosphatase10 (AtPAP10) as a Pi starvation-induced APase that is predominantly associated with the root surface. The AtPAP10 protein has phosphatase activity against a variety of substrates. Expression of AtPAP10 is specifically induced by Pi limitation at both transcriptional and posttranscriptional levels. Functional analyses of multiple atpap10 mutant alleles and overexpressing lines indicated that AtPAP10 plays an important role in plant tolerance to Pi limitation. Genetic manipulation of AtPAP10 expression may provide an effective means for engineering new crops with increased tolerance to Pi deprivation.

show abstract

“…Indeed, roots of living plants are known to secrete proteins. For example, large amounts of acid phosphatase are released from the roots of many plants during phosphate deficiency (40). We attempted to ''rhizosecrete'' the following three heterologous proteins of different origins from Nicotiana tabacum L.; green fluorescent protein (GFP) of the jellyfish Aequorea victoria, human placental secreted alkaline phosphatase (SEAP), and xylanase from the thermophylic bacterium Clostridium thermocellum.…”

mentioning

confidence: 99%

Use of plant roots for phytoremediation and molecular farming

Gleba

Borisjuk

et al. 1999

Proc. Natl. Acad. Sci. U.S.A.

229

View full text Add to dashboard Cite

Alternative agriculture, which expands the uses of plants well beyond food and fiber, is beginning to change plant biology. Two plant-based biotechnologies were recently developed that take advantage of the ability of plant roots to absorb or secrete various substances. They are (i) phytoextraction, the use of plants to remove pollutants from the environment and (ii) rhizosecretion, a subset of molecular farming, designed to produce and secrete valuable natural products and recombinant proteins from roots. Here we discuss recent advances in these technologies and assess their potential in soil remediation, drug discovery, and molecular farming.Biotechnology is transforming world agriculture, adding new traits to crop plants at a greatly accelerated rate. Plants are becoming more efficient producers of food, fiber, medicines, and construction materials. In addition to these conventional uses, biotechnology opens doors to unique uses of plants that are gaining greater acceptance from the public and attention from the scientific community. These so-called ''value-added'' uses include phytoremediation, the use of plants to remove pollutants from the environment or to render them harmless (1), and molecular farming (phytomanufacturing), the use of plants for the production of valuable organic molecules and recombinant proteins (2, 3). Because of the growing number of commercially successful applications and the lack of serious environmental concerns, both technologies are gaining acceptance from the scientific community, the general public, and regulators.With the exception of root crops, plant roots are less utilized and studied than shoots. However, this situation may be changing because of the emerging biotechnologies described below that exploit the ability of plants to transport valuable molecules into and out of their roots. These root-based technologies include metal phytoextraction, a subset of phytoremediation, which uses plants to remove toxic heavy metals from soil; and rhizosecretion, a subset of molecular farming, which relies on the ability of plant roots to exude valuable compounds. Both technologies exploit plants' innate biological mechanisms for human benefit.Phytoextraction. Giant underground networks formed by the roots of living plants function as solar-driven pumps that extract and concentrate essential elements and compounds from soil and water. Absorbed substances are used to support reproductive function and carbon fixation within shoots. Metal phytoextraction relies on metal-accumulating plants to transport and concentrate polluting metals, such as lead, uranium, and cadmium, from the soil into the harvestable aboveground shoots (1, 4, 5). Hydroponically grown plant roots can also directly absorb, precipitate, and concentrate toxic metals from polluted effluents in a process termed rhizofiltration (6).Chelate-assisted phytoextraction (1) has been successfully used to remove lead from contaminated soils using specially selected varieties of Indian mustard (Brassica juncea L.). These v...

show abstract

Comparison of Characteristics of Acid Phosphatases Secreted from Roots of Lupin and Tomato

Cited by 32 publications

References 32 publications

Characterization of Arabidopsis Acid Phosphatase Promoter and Regulation of Acid Phosphatase Expression

Characterization of Arabidopsis Acid Phosphatase Promoter and Regulation of Acid Phosphatase Expression

The Arabidopsis Purple Acid Phosphatase AtPAP10 Is Predominantly Associated with the Root Surface and Plays an Important Role in Plant Tolerance to Phosphate Limitation

Use of plant roots for phytoremediation and molecular farming

Contact Info

Product

Resources

About