Partial protection against classical swine fever virus elicited by dendrimeric vaccine-candidate peptides in domestic pigs

Tarradas, Joan; Monsò, Marta; Muñoz, Marta; Rosell, Rosa; Fraile, Lorenzo; Frı́as, Maria Teresa; Domingo, Mariano; Andreu, David; Sánchez‐Madrid, Francisco; Ganges, Llilianne

doi:10.1016/j.vaccine.2011.03.095

Cited by 46 publications

(35 citation statements)

References 36 publications

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“…The presence of antibodies in animals immunized with the peptide fragment with the ability to react against the whole E2 recombinant protein was seen, to our knowledge, for the first time in pigs. Conversely, very recent reports have suggested the use of this fragment in combination with others in order to increase the protective efficacy [73], [74]. Other authors for instance, give attention to peptides covering amino acids 829 to 837 or 844 to 865 used under more immunogenic presentations [72], [73], [75].…”

Section: Discussionmentioning

confidence: 99%

“…Whether this fragment could be the most immunogenic or fully protective against CSFV will require challenge trials in which more experimental groups, fragment combinations and displaying strategies are considered. One interesting issue is that with the use of one of these epitopes, cell-mediated specific immune responses appeared to correlate with partial protection in pigs in the absence of neutralizing antibodies [74]. Thus, an ideal strategy would consider one or more B-cell (and T-cell) epitope for presentation.…”

Section: Discussionmentioning

confidence: 99%

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Conformational and Thermal Stability Improvements for the Large-Scale Production of Yeast-Derived Rabbit Hemorrhagic Disease Virus-Like Particles as Multipurpose Vaccine

et al. 2013

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Recombinant virus-like particles (VLP) antigenically similar to rabbit hemorrhagic disease virus (RHDV) were recently expressed at high levels inside Pichia pastoris cells. Based on the potential of RHDV VLP as platform for diverse vaccination purposes we undertook the design, development and scale-up of a production process. Conformational and stability issues were addressed to improve process control and optimization. Analyses on the structure, morphology and antigenicity of these multimers were carried out at different pH values during cell disruption and purification by size-exclusion chromatography. Process steps and environmental stresses in which aggregation or conformational instability can be detected were included. These analyses revealed higher stability and recoveries of properly assembled high-purity capsids at acidic and neutral pH in phosphate buffer. The use of stabilizers during long-term storage in solution showed that sucrose, sorbitol, trehalose and glycerol acted as useful aggregation-reducing agents. The VLP emulsified in an oil-based adjuvant were subjected to accelerated thermal stress treatments. None to slight variations were detected in the stability of formulations and in the structure of recovered capsids. A comprehensive analysis on scale-up strategies was accomplished and a nine steps large-scale production process was established. VLP produced after chromatographic separation protected rabbits against a lethal challenge. The minimum protective dose was identified. Stabilized particles were ultimately assayed as carriers of a foreign viral epitope from another pathogen affecting a larger animal species. For that purpose, a linear protective B-cell epitope from Classical Swine Fever Virus (CSFV) E2 envelope protein was chemically coupled to RHDV VLP. Conjugates were able to present the E2 peptide fragment for immune recognition and significantly enhanced the peptide-specific antibody response in vaccinated pigs. Overall these results allowed establishing improved conditions regarding conformational stability and recovery of these multimers for their production at large-scale and potential use on different animal species or humans.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Conformational and Thermal Stability Improvements for the Large-Scale Production of Yeast-Derived Rabbit Hemorrhagic Disease Virus-Like Particles as Multipurpose Vaccine

et al. 2013

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“…Peptide vaccination is a key role of combining a good desirable immune response and a minimal immunological side effect. There are many peptide vaccines under development, such as vaccine for human immunodeficiency virus (HIV), hepatitis C virus (HCV), malaria, foot and mouth disease, swine fever, influenza, anthrax, human papilloma virus (HPV), therapeutic anti-cancer vaccines, pancreatic cancer, melanoma, non-small cell lung cancer, advanced hepatocellular carcinoma, cutaneous T-cell lymphoma and B-Cell chronic lymphocytic leukaemia [54][55][56][57][58][59][60][61][62][63][64][65][66][67].…”

Section: Discussionmentioning

confidence: 99%

Multi Epitope Peptide Vaccine Prediction against Sudan Ebola Virus Using Immuno-Informatics Approaches

Abu-haraz¹,

Abd-elrahman²,

Ibrahim³

et al. 2017

Adv Tech Biol Med

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Sudan Ebola virus is single stranded negative sense RNA genome belonging to Filovirus Filoviridae family that causes hemorrhagic fever. There is no treatment or vaccine for it, thus the aim of this study is to design a peptide vaccine using immuoinformatics approaches to analyse the glycoprotein of the all strain of SUDV, to determine the conserved region which is further studied to predict all possible epitopes that can be used as a peptide vaccine. A total of 21 Sudan Ebola virus glycoprotein retrieved from NCBI database were aligned to determine the conservancy and to predict the epitopes using IEDB analysis resource. Three epitopes predicted as a peptide vaccine for B cell (PPPPDGVR, ETFLQSPP, LQSPPIRE). For T cell four epitopes showed high affinity to MHC class I (FLYDRLAST, IIIAIIALL, MHNQNALVC and RTYTILNRK) and high coverage against Sudan and the whole world population. Also in MHC class II, Four epitopes that interact with most frequent MHC class II alleles (FAEGVIAFL, FLRATTELR, FLYDRLAST and FVWVIILFQ) with high coverage against Sudan and the whole world population. We recommend in vivo and in vitro study to prove the effectiveness of these predicted epitopes as a peptide vaccine.

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“…Peptide vaccines under various phases of trial and development, the vast majority of them related to cancers. [52][53][54][55][56][57][58][59][60][61][62][63][64].…”

Section: Discussionmentioning

confidence: 99%

Prediction and Conservancy Analysis of Multiepitope Based Peptide Vaccine Against Merkel Cell Polyomavirus: An Immunoinformatics Approach

Awad-Elkareem¹,

Osman²,

Mohamed³

et al. 2017

Immunome Res

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Merkel cell Polyomavirus is non-enveloped, dsDNA virus belonging to Polyomaviridae family linked to an uncommon aggressive skin malignancy. The poor prognosis and limited understanding of disease pathogenesis warrants innovative treatment. In this current study we aim to predict TB cell immunogenic epitopes from the VP1 protein of all merkel cell polyomavirus strain which will aid in effective epitope based vaccine design using immuoinformatics approaches. We retrieved 423 full-length VP1 protein sequences of merkel cell polyomavirus species from the NCBI database. These sequences were analyzed to determine the conserved region and were used to predict the epitopes using the IEDB immunoinformatics algorithms. For B cell three epitope were predicted as peptide vaccine (QEKTVY, KTVYPK, and QEKTVYP). For T cell the predicted Class-I peptides (SLFSNLMPK, LQMWEAISV and LLVKGGVEV) were found to cover the maximum number of MHC I alleles. The highest scoring Class II MHC binding peptides were (IELYLNPRM, ISSLINVHY and INSLFSNLM). Further experiments will need to be undertaken to confirm the potential of these predicted epitopes in a future efficacious vaccine development.

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Partial protection against classical swine fever virus elicited by dendrimeric vaccine-candidate peptides in domestic pigs

Cited by 46 publications

References 36 publications

Conformational and Thermal Stability Improvements for the Large-Scale Production of Yeast-Derived Rabbit Hemorrhagic Disease Virus-Like Particles as Multipurpose Vaccine

Conformational and Thermal Stability Improvements for the Large-Scale Production of Yeast-Derived Rabbit Hemorrhagic Disease Virus-Like Particles as Multipurpose Vaccine

Multi Epitope Peptide Vaccine Prediction against Sudan Ebola Virus Using Immuno-Informatics Approaches

Prediction and Conservancy Analysis of Multiepitope Based Peptide Vaccine Against Merkel Cell Polyomavirus: An Immunoinformatics Approach

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