Partial loss-of-function alleles reveal a role for<i>GNOM</i>in auxin transport-related, post-embryonic development of<i>Arabidopsis</i>

Geldner, Niko; Richter, Sandra; Vieten, Anne; Marquardt, Sebastian; Torres-Ruiz, Ramón A.; Mayer, Ulríke; Jürgens, Gerd

doi:10.1242/dev.00926

Cited by 263 publications

(304 citation statements)

References 35 publications

(42 reference statements)

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“…6A). Similar to the GNOM function during lateral root initiation, where GNOM is required for PIN1 repolarization and the redirection of auxin flow (Geldner et al, 2004), epidermal GNOM expression in late-globular embryos may also reorient auxin flow for cotyledon initiation. The importance of the epidermis in cotyledon formation has already been implicated by several other studies (Abe et al, 2003;Benkova et al, 2003), but this study is the first direct link between epidermal auxin flow and cotyledon separation.…”

Section: Gnom-dependent Organ Formation and Symmetry Transition In Thmentioning

confidence: 97%

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Coordination of apical and basal embryo development revealed by tissue-specific GNOM functions

et al. 2011

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SUMMARYFlowering-plant embryogenesis generates the basic body organization, including the apical and basal stem cell niches, i.e. shoot and root meristems, the major tissue layers and the cotyledon(s). gnom mutant embryos fail to initiate the root meristem at the early-globular stage and the cotyledon primordia at the late globular/transition stage. Tissue-specific GNOM expression in the gnom mutant embryo revealed that both apical and basal embryo organization depend on GNOM provascular expression and a functioning apical-basal auxin flux: GNOM provascular expression in gnom mutant background resulted in non-cell-autonomous reconstitution of apical and basal tissues which could be linked to changes in auxin responses in those tissues, stressing the importance of apical-basal auxin flow for overall embryo organization. Although reconstitution of apical-basal auxin flux in gnom results in the formation of single cotyledons (monocots), only additional GNOM epidermal expression is able to induce wild-type apical patterning. We conclude that provascular expression of GNOM is vital for both apical and basal tissue organization, and that epidermal GNOM expression is required for radial-to-bilateral symmetry transition of the embryo. We propose GNOM-dependent auxin sinks as a means to generate auxin gradients across tissues.

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Section: Gnom-dependent Organ Formation and Symmetry Transition In Thmentioning

confidence: 97%

“…Similar to the GNOM promoter (see Fig. S1A-D in the supplementary material) (Geldner et al, 2004) the RPS5a promoter is expressed in all tissues during early embryogenesis (see Fig. S1E-F in the supplementary material) (Weijers et al, 2001).…”

Section: Rps5a>>gnom Fully Complements the Gnom Mutant Phenotypementioning

confidence: 99%

Coordination of apical and basal embryo development revealed by tissue-specific GNOM functions

et al. 2011

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“…N-1-naphthylphthalamic acid (NPA) applications on hypocotyls were performed as described (Geldner et al, 2004), during 3 days in light or 4 days in darkness. For lactacystin treatments, 5-day-old seedlings were incubated for 4 hours, in light or in darkness, with 50 M lactacystin or DMSO as mock.…”

Section: Chemical Treatmentsmentioning

confidence: 99%

COP1 mediates the coordination of root and shoot growth by light through modulation of PIN1- and PIN2-dependent auxin transport in Arabidopsis

et al. 2012

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SUMMARYWhen a plant germinates in the soil, elongation of stem-like organs is enhanced whereas leaf and root growth is inhibited. How these differential growth responses are orchestrated by light and integrated at the organismal level to shape the plant remains to be elucidated. Here, we show that light signals through the master photomorphogenesis repressor COP1 to coordinate root and shoot growth in Arabidopsis. In the shoot, COP1 regulates shoot-to-root auxin transport by controlling the transcription of the auxin efflux carrier gene PIN-FORMED1 (PIN1), thus appropriately tuning shoot-derived auxin levels in the root. This in turn directly influences root elongation and adapts auxin transport and cell proliferation in the root apical meristem by modulating PIN1 and PIN2 intracellular distribution in the root in a COP1-dependent fashion, thus permitting a rapid and precise tuning of root growth to the light environment. Our data identify auxin as a long-distance signal in developmental adaptation to light and illustrate how spatially separated control mechanisms can converge on the same signaling system to coordinate development at the whole plant level.

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“…Vesicles carrying auxin efflux carriers of the PIN family are retargeted in a polar manner to a specific side of the cell membrane (Boutté et al, 2006). In this process of retrograde endocytosis, GNOM (ADP-ribosylation factor -guanine nucleotide exchange factor) acts specifically at the assembly of vesicles containing PIN1 (Geldner et al, 2004), and GNL1 (GNOM-LIKE1) controls the trafficking of PIN2 (Kleine-Vehn et al, 2008). Both GNOM and GNL1 control auxin polar transport and at the same time are auxin-dependent Nick, Han, and An, 2009).…”

Section: Actin Cytoskeleton Organizationmentioning

confidence: 99%

Visualization and analysis of actin cytoskeleton organization in plants

Pozhvanov

2018

BioComm

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The plant cytoskeleton is a highly dynamic system that consists of two components: microfilaments and microtubules. Actin microfilaments are essential for polar growth, cytoplasmic streaming, directing polar growth, anchoring the nucleus, gravity sensing, signalling pathway integration and a number of other functions. Actin morphology and dynamics are orchestrated by a variety of small actin binding proteins, and some of them have become a source of actin interaction domains widely used as markers for microfilaments in fusions with fluorescent reporter proteins. However, older techniques are still employed for actin visualization. In this short review, we will focus on the diversity of fluorescent reporter fusions for F-actin and on approaches and existing free software for the analysis of cytoskeleton organization, mainly in Arabidopsis.

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Partial loss-of-function alleles reveal a role forGNOMin auxin transport-related, post-embryonic development ofArabidopsis

Cited by 263 publications

References 35 publications

Coordination of apical and basal embryo development revealed by tissue-specific GNOM functions

Coordination of apical and basal embryo development revealed by tissue-specific GNOM functions

COP1 mediates the coordination of root and shoot growth by light through modulation of PIN1- and PIN2-dependent auxin transport in Arabidopsis

Visualization and analysis of actin cytoskeleton organization in plants

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