Partial hepatectomy and mediators of inflammation decrease the expression of liver α₂‐HS glycoprotein gene in rats

Abstract

Liver mRNA levels of two acute phase reactant (APR) proteins, %-HS glycoprotein (a major negative APR) and q-acid glycoprotein (a major positive APR) were measured in male rats at different times after the administration of turpentine, of tumor necrosis factor, or following partial hepatectomy. In every case, a marked decrease in mRNA levels of a;-HS glycoprotein was observed which reached a maximum at 24 h. A concomitant increase of q-acid glycoprotein mRNA levels was observed under the same conditions. These results indicate that the decreasedlevels of d;-HS glycoprotein induced by the acute-phase response following inflammatory mediators and partial hepatectomy are due to a down-regulation of the gene expression of this protein in rat liver.

Fetuin-B, a second member of the fetuin family in mammals

“…A time-course study of these hepatic mRNA levels following a TO challenge is shown in Figure 3. The fetuin-A mRNA level was transiently down-regulated as expected from former reports [23,36] while the mRNA level for a positive APP used as a control, namely the IαIH4P protein of the Inter-α-Inhibitor family, was transiently up-regulated as described previously [37]. The level of fetuin-B mRNA was down-regulated as early as 9 h post-TO, reached a 2-fold decrease at days 1-2 and returned to a normal value beyond 1 week, which on the whole is similar to the variations seen for the fetuin-A mRNA level.…”

“…The hepatic level of fetuin-A mRNA is down-regulated during an APR in rat [23,26] and our preliminary data indicated that the hepatic level of fetuin-B mRNA also varies following an inflammatory challenge in rat [27]. Therefore, we compared in more detail the changes of both mRNA levels of both genes in liver during an APR in rat.…”

“…Indeed, human AHSG, rat pp63 and guinea pig fetuin belong to the group of so-called negative acute-phase proteins (APPs) that are down-regulated during APR, whereas mouse and cattle fetuins are up-regulated, i.e. are positive APPs [8,[23][24][25]. Given the numerous suggested functions of fetuins, it is important to have a complete view of this protein family.…”

cDNA Sequencing of Guinea Pig a2-HS Glycoprotein, Its Expression in Various Tissues and Acute Phase Expression

“…Protein and/or DNA sequences have been reported for human (Lee et al, 1987), cow (Dziegielewska et al, 1990), sheep and pig , rat (Ohnishi et al, 1993), mouse (Yamamoto and Sinohara, 1993;Yang et al, 1992), Mongolian gerbil (Goto et al, 1997), rabbit (Osawa et al, 1997), and Habu snake (Yamakawa and Omori-Satoh, 1992) fetuins. AHSG is also one of the few negative acute phase reactants in humans (Lebreton et al, 1979) and rats (Daveau et al, 1990), whereas fetuin is a positive acute phase protein in cattle and mice (Yang et al, 1992). To clarify the physiological role of AHSG, the structure and properties should be studied in more animal species and compared.…”

Analysis of human serum glycoproteins in patients with acute coronary syndrome

“…Nồng độ của AHSG trong huyết thanh thường giảm đi khi xuất hiện các triệu chứng viêm nhiễm hay các khối u ác tính [2]. Các nghiên cứu sử dụng phương pháp phân tích hóa sinh truyền thống đã cho thấy, sự giảm của nồng độ protein này ở bệnh nhân động mạch vành [10].…”