Partial filling affinity capillary electrophoresis using large-volume sample stacking with an electroosmotic flow pump for sensitive profiling of glycoprotein-derived oligosaccharides

Fukushima, Eriko; Yagi, Yuki; Yamamoto, Sachio; Nakatani, Yumi; Kakehi, Kazuaki; Hayakawa, Tetsuo; Suzuki, Shigeo

doi:10.1016/j.chroma.2012.02.052

Cited by 30 publications

(17 citation statements)

References 42 publications

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“…43 Fukushima et al combined LVSEP with partial filling affinity capillary electrophoresis (PFACE) to realize a highly sensitive analysis of the interaction of glycoprotein-derived oligosaccharides with some plant lectins. 46 By injecting lectin from the anodic end as a short plug for PFACE, followed by the injection of a sample solution to the entire capillary for LVSEP, anionic APTS-labeled oligosaccharides were continuously moved and stacked in the lectin plug until completion of the preconcentration, which would enhance the interaction efficiency of oligosaccharides with lectins. In PFACE-LVSEP, a PDMS-coated capillary and a BGS containing hydroxypropylcellulose (HPC) was used to suppress the EOF and analytes/lectin surface adsorption.…”

Section: Lvsep Combined With Cd-and Affinity-based Separation Modesmentioning

confidence: 99%

On-line Sample Preconcentration by Large-volume Sample Stacking with an Electroosmotic Flow Pump (LVSEP) in Microscale Electrophoresis

2013

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Section: Lvsep Combined With Cd-and Affinity-based Separation Modesmentioning

confidence: 99%

On-line Sample Preconcentration by Large-volume Sample Stacking with an Electroosmotic Flow Pump (LVSEP) in Microscale Electrophoresis

2013

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“…Kawai et al examined the influence of the addition of CD to the BGE on the LVSEP pre‐concentration of ibuprofen in urine, which was desalted by SPE with a C18 column. LVSEP has also been used for the analysis of oligosaccharides and in combination with partial‐filling affinity CE (PF‐ACE) for profiling of glycoprotein‐derived oligosaccharides . In microchips, Kawai et al used LVSEP for the determination of natural polyphenols in plant extracts and were able to achieve a sensitivity enhancement of up to 2200‐ to 2900‐fold when compared to conventional microchip electrophoresis.…”

Section: Stackingmentioning

confidence: 99%

Recent advances in enhancing the sensitivity of electrophoresis and electrochromatography in capillaries and microchips (2010–2012)

et al. 2013

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CE has been alive for over two decades now, yet its sensitivity is still regarded as being inferior to that of more traditional methods of separation such as HPLC. As such, it is unsurprising that overcoming this issue still generates much scientific interest. This review continues to update this series of reviews, first published in Electrophoresis in 2007, with updates published in 2009 and 2011 and covers material published through to June 2012. It includes developments in the field of stacking, covering all methods from field amplified sample stacking and large volume sample stacking, through to isotachophoresis, dynamic pH junction and sweeping. Attention is also given to online or inline extraction methods that have been used for electrophoresis.

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“…This technique can be used to specifically detect oligosaccharides in a complex mixture. [40,41] 'Large-volume sample stacking with an electroosmotic flow pump' (LVSEP) is a preconcentration technique used for the analysis of analytes introduced in the whole of a separation capillary [42]. The method seems useful in PFACE for the analysis in minute amount of glycans in complex mixture.…”

Section: Multiple-injection Analysesmentioning

confidence: 99%

Highly Sensitive Methods Using Liquid Chromatography and Capillary Electrophoresis for Quantitative Analysis of Glycoprotein Glycans

Suzuki

2014

Chromatography

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This review summarizes our recent works concerned on the analysis of glycoprotein glycans. Glycoprotein glycans are highly complicated and have variations that number into the thousands. Therefore the method for their analyses should be carefully chosen according to the complexity and quantity of glycans in samples. To shorten the time for the preparation of labeled glycans from glycoprotein, we proposed two methods: PNGase F/NBD-F method enables the preparation of fluorescently labeled glycans from glycoproteins about 2 hr. A combination of a neutrally coated capillary and borate buffer eliminates the purification steps for the removal of excess reagents from the glycan samples for capillary electrophoresis. Partial filling affinity capillary electrophoresis using glycan-recognizing proteins such as lectins, glycosidases and immunoglobulins, was used for the structural analysis of glycans. The method was applied to the specific detection of NeuGc and α-Gal containing glycans known as the heterogenic antigens. Sensitivity of the detection of glycoprotein glycans separated by microchip-based electrophoresis was enhanced by in situ fabricated sufonate-type polyacrylamide gel. This method enhances the sensitivity by a factor of 10 5 . In situ fabrication of lectin impregnated gels enables specific entrapment and analysis of glycans. Conversion reaction from fluorescently labeled glycans (1-amino-1-alditols) to free oligosaccharides will be helpful for the functional analysis of glycans.

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Partial filling affinity capillary electrophoresis using large-volume sample stacking with an electroosmotic flow pump for sensitive profiling of glycoprotein-derived oligosaccharides

Cited by 30 publications

References 42 publications

On-line Sample Preconcentration by Large-volume Sample Stacking with an Electroosmotic Flow Pump (LVSEP) in Microscale Electrophoresis

On-line Sample Preconcentration by Large-volume Sample Stacking with an Electroosmotic Flow Pump (LVSEP) in Microscale Electrophoresis

Recent advances in enhancing the sensitivity of electrophoresis and electrochromatography in capillaries and microchips (2010–2012)

Highly Sensitive Methods Using Liquid Chromatography and Capillary Electrophoresis for Quantitative Analysis of Glycoprotein Glycans

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