Journal of Chromatography A 2012 DOI: 10.1016/j.chroma.2012.02.052 View full text
Eriko Fukushima, Yuki Yagi, Sachio Yamamoto, Yumi Nakatani, Kazuaki Kakehi, Takao Hayakawa, Shigeo Suzuki

Abstract: An online preconcentration technique, large-volume sample stacking with an electroosmotic flow pump (LVSEP) was combined with partial filling affinity capillary electrophoresis (PFACE) to realize highly sensitive analysis of the interaction of glycoprotein-derived oligosaccharides with some plant lectins. Oligosaccharides derivatized with 8-aminopyrene-1,3,6-trisulfonic acid (APTS) were delivered to an entire neutrally-coated capillary and then lectin solution was hydrodynamically introduced from the outlet of…

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