Partial expression of monoaminergic (serotoninergic) properties by the multipotent hypothalamic cell line F7. An example of learning at the cellular level

Vitry, F. De; Catelon, J.; Dubois, M. P.; Thibault, Jean; Barritault, Denis; Courty, José; Bourgoin, S.; Hamon, Michel

doi:10.1016/0197-0186(86)90030-6

Cited by 11 publications

(6 citation statements)

References 36 publications

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“…These results also support and extend the conclusion of our studies (4) that showed that under appropriate culture conditions, repetitive treatment with a 5-HT agonist and eyederived growth factor, irreversibly induced AA decarboxylase activity in the primitive hypothalamic cell line F7: such induction, reinforcement, and stabilization of the commitment of one cell have been defined as a "learning" process at the cellular level (4). The capacity of embryonic nerve cells to synthesize and respond to their own neurotransmitter, and the stimulatory effect of a neurotransmitter, such as 5-HT, on the neuron commitment to a given neurochemical specificity, could be an important concept in determining the functional link between neurotransmitters and brain development.…”

Section: Discussionsupporting

confidence: 79%

“…Antibodies to formaldehyde-linked 5-HT-bovine serum albumin conjugates were raised in rabbits and purified as described (12). The 5-HT antiserum presently used exhibited no crossreactivity with 5-HTP, 8-OH-DPAT, dopamine, norepinephrine, or numerous other related molecules (4,12). Before use, the 5-HT antiserum was preadsorbed for 1 day with 0.2% bovine serum albumin.…”

Section: Methodsmentioning

confidence: 99%

“…Identification of serotoninergic neuron-like cells. For brain stem cultures, 5-HT immunoreactivity was detected using the immunoperoxidase technique as described (4). In the case of hypothalamic cultures, cells were first treated with 1 ,uM 5-HTP for 2 hr at 37°C, then fixed and assayed for 5-HT immunoreactivity.…”

Section: Methodsmentioning

confidence: 99%

“…Initially we used the pluripotent embryonic hypothalamic nerve cell line F7 to initiate distinct neuronal or glial functions by establishing appropriate external signals in the culture environment (3, 4). Thus, it was possible to induce serotonin (5-hydroxytryptamine or 5-HT) synthesis from 5-hydroxytryptophan, L-form, (5-HTP) by repetitive treatment ofthe F7 cell line with a 5-HT agonist and eye-derived growth factor in appropriate culture conditions (4). In this particular case, an autocrine-like mechanism seemed to be involved in nerve cell differentiation.…”

mentioning

confidence: 99%

“…Direct biochemical measurement of 5-HTP decarboxylation in cultures exposed to 8-OH-DPAT (as that performed with the primitive nerve cell line F7, ref. 4) was not possible as the substrate 3H-labeled 5-HTP is no longer available from any commercial source. Nevertheless, immunocytochemical data were highly reproducible using cultures from ED 14 embryos, and the same developmental stage was chosen for further characterization of the possible effects of 8-OH-DPAT upon 5-HT uptake and accumulation in hypothalamic cells.…”

mentioning

confidence: 99%

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Serotonin initiates and autoamplifies its own synthesis during mouse central nervous system development.

Vitry¹,

Hamon²,

Catelon³

et al. 1986

Proc. Natl. Acad. Sci. U.S.A.

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Some cells from cultured embryonic mouse hypothalamus were found to express aromatic-L-amino acid decarboxylase (EC 4.1.1.28) activity and serotonin uptake and storage. These neuron-like cells differed from serotoninergic neurons in cultured embryonic mouse brain stem since they did not contain tryptophan hydroxylase. We studied the effect of the serotonin agonist 8-hydroxy-2-[di-(n-propyl)amino]tetralin on neuronal differentiation of hypothalamic cells from 12-to 15-day embryos. Repeated treatment of cultures with the serotonin agonist for 10 days resulted in an increased number of serotonin cells containing high levels of decarboxylase activity. Both the increase in cell numbers and the elevated decarboxylase activity could be suppressed by the addition of the serotonin antagonist metergoline to the culture medium. These data show that serotonin (or an agonist), acting on specific receptors, can initiate and amplify its own synthesis in embryonic hypothalamic neurons, as observed in the primitive hypothalamic nerve cell line F7

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Section: Discussionsupporting

confidence: 79%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

See 3 more Smart Citations

Serotonin initiates and autoamplifies its own synthesis during mouse central nervous system development.

Vitry¹,

Hamon²,

Catelon³

et al. 1986

Proc. Natl. Acad. Sci. U.S.A.

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Development and lesion induced reorganization of the cortical representation of the rat's body surface as revealed by immunocytochemistry for serotonin

Rhoades

Bennett‐Clarke

Chiaia

et al. 1990

J of Comparative Neurology

130

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Immunocytochemistry with an antiserum directed against serotonin (5-HT) was used to assess the development of the representation of the body surface in the rat's primary somatosensory cortex (S-I). Within 1 hour of birth (P-O), 5-HT-positive fibers were present in the marginal zone, the cortical plate, and developing layers V and VI. Immunoreactivity in the marginal zone consisted of a thin band of coarse fibers oriented parallel to the pia. Only a small number of isolated fibers were visible in the cortical plate. A denser network of both coarse and fine fibers could be seen in presumptive layers V and VI. By the first hour of P-I, 5-HT-positive axons in the deeper cortical plate were organized into a crude representation of the rat's body surface. At this age, aggregates of fibers corresponding to the head, lower jaw, trunk, and forepaw could be clearly distinguished. These regions of dense 5-HT immunoreactivity consisted primarily of fine caliber axons that had invaded the lower part of the cortical plate. Dense aggregates of fine caliber axons were also visible in developing layers V and VI. Coarse 5-HT-positive fibers were visible in all layers, but they did not appear to contribute to the pattern that corresponded to the body surface. By the first hour of P-2, the map of the body surface in S-I was more refined and a row-related organization of 5-HT-immunoreactive fibers was visible in the portion of the cortex representing the vibrissa pad. The laminar distributions of coarse and fine caliber serotoninergic axons at this age were essentially the same as on P-I. By P-2.5 (60 hours after birth), patches of 5-HT-positive fibers corresponding to individual vibrissa follicles were clearly evident. These consisted of dense aggregates of fine caliber axons that were centered in presumptive layer IV, but which also extended above and below this lamina. Over the next 3 days, the pattern continued to mature. By P-4, dense 5-HT labelling was also visible in the secondary somatosensory cortex (S-II). By the beginning of P-5, clusters of fibers corresponding to more rostral facial hairs and individual digits within the forepaw representation could also be discerned. By P-12, the differential distribution of 5-HT fibers in S-I was no longer visible. Thus, immunocytochemistry for serotonin showed a representation in S-I homeomorphic with the body surface prior to the age at which it can be discerned with other methods thought to reveal thalamocortical axons. Transection of the infraorbital nerve (ION) on the day of birth altered the organization of the vibrissal representation in the contralateral cortex from the earliest age at which it could be detected by 5-HT immunocytochemistry in normal animals. However, the departure from the normal organization was gradual. Row-related organization was clearly visible in the cortices of rats sacrificed on P-3, but not in those of rats that were killed on P-5. These results suggested that the organization of the 5-HT innervation of the cortex may be guided by thalamic afferents an...

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Serotonergic innervation of the lateral geniculate nucleus of the rat during postnatal development: A light and electron microscopic immunocytochemical analysis

Dinopoulos

Dori

Parnavelas

1995

J of Comparative Neurology

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The serotonergic innervation of the developing lateral geniculate nucleus of the rat was studied with immunocytochemical techniques at the light and electron microscope levels. A relatively small number of thick serotonergic fibers were observed at the time of birth, distributed more densely in the ventral portion of the nucleus and in the intergeniculate leaflet than in the dorsal lateral geniculate nucleus. By the end of the first postnatal week, this distribution pattern was more clearly established, but the number of immunoreactive fibers was increased. Thereafter, and until the adult pattern was established at the end of the third postnatal week, serotonergic fibers increased further in number and changed morphologically (e.g., they became finer and more ramified with closely spaced varicosities), but their pattern of distribution remained unchanged. Electron microscopical analysis of the dorsal lateral geniculate nucleus revealed that the vast majority of serotonin varicosities formed asymmetrical synapses with dendritic shafts; axosomatic synapses were a feature of the nucleus only at the time of birth. The proportion of serotonin varicosities forming synapses increased gradually from birth to reach a peak at the end of the second postnatal week, then declined markedly in the following week before increasing again at a later stage. It may be speculated that synapses formed during the first two weeks of life may be related to the involvement of serotonin in the morphogenesis of the lateral geniculate nucleus, whereas those formed later in development may be involved in the mediation of neurotransmitter effects.

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Partial expression of monoaminergic (serotoninergic) properties by the multipotent hypothalamic cell line F7. An example of learning at the cellular level

Cited by 11 publications

References 36 publications

Serotonin initiates and autoamplifies its own synthesis during mouse central nervous system development.

Serotonin initiates and autoamplifies its own synthesis during mouse central nervous system development.

Development and lesion induced reorganization of the cortical representation of the rat's body surface as revealed by immunocytochemistry for serotonin

Serotonergic innervation of the lateral geniculate nucleus of the rat during postnatal development: A light and electron microscopic immunocytochemical analysis

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