Partial Excision of the Chromosomal Cassette Containing the Methicillin Resistance Determinant Results in Methicillin-Susceptible<i>Staphylococcus aureus</i>

Donnio, Pierre Yves; Oliveira, Duarte C.; Faria, Natália; Wilhelm, Nathalie; Coustumier, Alain Le; Lencastre, Hermı́nia de

doi:10.1128/jcm.43.8.4191-4193.2005

Cited by 77 publications

(54 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The outbreak strain differs from USA300 MRSA only by an approximately 30-kb deletion consistent with loss of SCCmec IV. Our isolate is similar to USA300 MSSA strains previously described in nonoutbreak-associated settings (3,10,13).…”

supporting

confidence: 50%

Outbreak of Skin Infections in College Football Team Members Due to an Unusual Strain of Community-Acquired Methicillin-Susceptible Staphylococcus aureus

et al. 2010

View full text Add to dashboard Cite

show abstract

supporting

confidence: 50%

Outbreak of Skin Infections in College Football Team Members Due to an Unusual Strain of Community-Acquired Methicillin-Susceptible Staphylococcus aureus

et al. 2010

View full text Add to dashboard Cite

show abstract

“…In the latter case, some elements are left behind at the ISS. Partial excision of SCCmec from the chromosome of a HA-MRSA strain may result in MSSA with a retained resistance to antibiotics other than β-lactam antibiotics [75]. This phenomenon has previously been described in studies where a high proportion of the investigated MSSA isolates were considered to derive from MRSA with partial excision of SCCmec [76][77].…”

Section: Sccmecmentioning

confidence: 89%

Epidemiological and molecular biological studies of multi-resistant methicillin-susceptible Staphylococcus aureus

Lindqvist¹

2014

View full text Add to dashboard Cite

Antibiotic resistance is increasingly recognised as a major problem and threat. During the last decades Gram-positive bacteria in general, and methicillin-resistant Staphylococcus aureus (MRSA) in particular, have been in focus both concerning matters of antibiotic resistance and as pathogens causing health care-associated (nosocomial) infections. In contrast to MRSA, studies on clonal distribution of methicillin-susceptible S. aureus (MSSA) are scarce.However, interest in MSSA has increased since it was shown that MRSA emerges from susceptible backgrounds by acquisition of a staphylococcal cassette chromosome element, carrying the mecA gene encoding methicillin-resistance (SCCmec).In an outbreak investigation of MRSA in Östergötland County, Sweden, in 2005, a high incidence of MSSA isolates with concomitant resistance to erythromycin, clindamycin and tobramycin (ECT-R) was detected. Analysis showed that 91 % of the investigated isolates were genetically related (clonal). The ECT-R clone was divided into four different but closely related patterns with pulsed-field gel electrophoresis (PFGE), and was designated spa type t002. Whole genome sequencing revealed that the ECT-R clone carried a pseudo-SCC element estimated to be 12 kb in size, showing a resemblance of more than 99 % with the SCCmec type II element of MRSA strain N315 (New York/Japan clone). This suggested a probable derivation from a highly successful MRSA strain, which had partially excised its SCCmec. The clonal outbreak was concentrated in eight hospital departments and two primary care centres, all located in the city of Linköping. Despite a high exchange of patients with the hospitals in the neighbouring counties in southeast Sweden (Jönköping-and Kalmar County), the ECT-R clone seemed to be limited to Östergötland County. However, a tobramycin-resistant clone predominated by isolates of spa type t084 was found in all three counties in southeast Sweden, and in particular among newborns, suggesting inter-hospital transmission.The ECT-R clone has survived as an abundant MSSA clone for a decade in Östergötland County, which indicates an insufficiency in the maintenance of basic hygiene guidelines, and that the clone probably possesses mechanisms of virulence and transmission that are yet to be discovered. Sammanfattning på svenska

show abstract

“…Baddour (2007) confirmed in his study that MSRA strains have been implicated in serious infections and nosocomial infectious, the strains being detected with several conventional methods. Following the affirmation that MRSA is considered the main cause of nosocomial infections prevalence, Donnio (2005) demonstrated that in French hospitals, MRSA strains increased until that time of the study. As well as Dickinson (2000) confirmed that Staphylococcus aureus clinical isolates tend to be either very susceptible or very resistant to methicillin.…”

Section: Clinical Isolatesmentioning

confidence: 99%

“…Accessory genome or dispensable genetic material of staphylococci bacteria contains virulence factors such as chromosomal cassettes (Donnio et al, 2005), pathogenicity islands (Che et al, 2014), genomic islands (Juhas et al, 2008), bacteriophages (Orlova, 2012), plasmids and transposons (Bennet, 2008). These contain the responsible genes for resistance to antibiotics, like mecA gene which is responsible for resistance to methicillin (Singh et al, 2006;Ferry et al, 2009).…”

Section: Clinical Isolatesmentioning

confidence: 99%

“…These contain the responsible genes for resistance to antibiotics, like mecA gene which is responsible for resistance to methicillin (Singh et al, 2006;Ferry et al, 2009). Transmission of these mobile genetic elements of a staphylococci strain to another one is realized through horizontal gene transfer as described by Donnio et al (2005). Methicillin resistant Staphylococcus (MRS) is considered the main cause of nosocomial infections prevalence.…”

Section: Clinical Isolatesmentioning

confidence: 99%

See 1 more Smart Citation

Molecular Level Investigation of Staphylococci’s Resistance Mechanisms to Antibiotics

Pruteanu¹,

Jäntschi

Crăciunaş

2017

Not Sci Biol

View full text Add to dashboard Cite

Polymerase chain reaction (PCR) techniques development allows the elaboration of many assays for identification of bacteria's resistance mechanisms to antibiotics. Following this idea, the results of molecular level investigation of bacteria's resistance mechanisms to antibiotics may give many opportunities to find more rapid methods for identifying the genes which are responsible for antibiotic resistance induction. The aim of the current study was to investigate antibiotic resistance genes in Staphylococcus bacteria on molecular level. Among classes of antibiotics, macrolides-lincosamides-streptogramin B (MLSB) and beta-lactams were used. In the proposed study, the bacterial strains were represented by 50 isolates of Staphylococcus. The bacterial strains were analyzed using polymerase chain reaction to identify the nuc, tuf, tst, sea, pathogenic activity genes. Thereafter, the bacteria were tested for ermA, ermB, ermC genes and for mecA, femA which are involved in the resistance to macrolides, lincosamides, streptogramin B and to beta-lactams, respectively. The presence or the absence of these genes confirmed that tested strains are resistant to specific antibiotic or not. Bacteria pathogenic activity was emphasized by genes as follows: sea (enterotoxin) which was found at all isolates, tst (toxic shock toxin) gene was not detected in any of the isolates and tuf gene (elongation factor) was obtained with one pair of primers. Resistance to beta-lactams was evidenced by the presence of mecA in all isolates and femA in some strains. Each of ermC, ermA and ermB, macrolides-lincosamides-streptogramin B resistance genes, was detected.

show abstract

Partial Excision of the Chromosomal Cassette Containing the Methicillin Resistance Determinant Results in Methicillin-SusceptibleStaphylococcus aureus

Cited by 77 publications

References 27 publications

Outbreak of Skin Infections in College Football Team Members Due to an Unusual Strain of Community-Acquired Methicillin-Susceptible Staphylococcus aureus

Outbreak of Skin Infections in College Football Team Members Due to an Unusual Strain of Community-Acquired Methicillin-Susceptible Staphylococcus aureus

Epidemiological and molecular biological studies of multi-resistant methicillin-susceptible Staphylococcus aureus

Molecular Level Investigation of Staphylococci’s Resistance Mechanisms to Antibiotics

Contact Info

Product

Resources

About