Partial characterization of extracellular polysaccharides produced by cyanobacterium Arthrospira platensis

Abstract

In this study, the physico-chemical characteristics of extracellular polysaccharides (EPS) produced by Arthrospira platensis were evaluated. Elemental analysis and a bicinchoninic acid (BCA) reaction indicated that the EPS were heteropolysaccharides that contain carbohydrate (13%) and protein (55%) moieties. Analysis of the infrared spectrum and elemental analysis revealed the presence of a sulfate group (0.5%). The UV-visible spectrum showed high UV absorption at 190~230 nm and a shoulder at 260~280 nm. In addition, this spectrum indicated that EPS can form aggregates with mycosporine-like amino acids and/or scytonemin. Gas chromatography analysis of the carbohydrate portion of the EPS indicated that it was composed of seven neutral sugars: galactose (14.9%), xylose (14.3%), glucose (13.2%), frucose (13.2%), rhamnose (3.7%), arabinose (1%), and mannose (0.3%) and two uronic acids, galacturonic acid (13.5%) and glucuronic acid (0.9%). © KSBB

Isolation, characterization and localization of extracellular polymeric substances from the cyanobacteriumArthrospira platensisstrain MMG-9

“…The relative proportion of fucose in EPS of Nostoc has been associated with the salt resistance of this cyanobacterium (Yoshimura et al, 2012). Xylose has also been reported as an important component of EPS (Filali Mouhim et al, 1993;Trabelsi et al, 2009), supporting our finding of the large proportion of this sugar in the TEPS of A. platensis (23%), although previous studies of TEPS have not reported the rare sugar ribose as was found in this study. Confocal laser scanning microscopy provided information with regard to the localization of EPS and supported the view that the different operational EPS fractions could indeed be related to recognizable structures (Ruas-Madiedo & de los Reyes-Gavilan, 2005).…”

“…One speciality of cyanobacterial EPS is the presence of one to three pentose sugars, which differentiate cyanobacterial EPS from other microbial EPS (Sutherland, 1994). The composition of EPS of A. platensis is complex (Trabelsi et al, 2009). Quantitative analysis of EPS fractions considered in this study, i.e.…”

“…It is one of the best-studied and most widely used cyanobacteria in biotechnology because of its fast growth in open ponds under alkaline conditions that largely exclude contaminating organisms (Ahmed et al, 2010b). Arthrospira platensis has enormous potential for applications in a variety of fields such as food, cosmetics, environmental improvement, aquaculture and in the future perhaps therapeutics (Rossi et al, 2005;Trabelsi et al, 2009). …”

“…As is the case for many other cyanobacteria, Arthrospira secretes a variety of different EPS that can be extracted and used in different fields (Trabelsi et al, 2009), for instance, arthrospiral EPS has been reported to enhance human innate immunity (Lobner et al, 2008;Parages et al, 2012). The objective of the present work was (1) to extract different EPS fractions and characterize them biochemically and (2) to visualize the EPS of A. platensis without disturbing its structural integrity by using various lectins and confocal laser scanning microscopy.…”

Functionalization of Alginate via Phosphorization: Preparation, Characterization.

“…A shoulder was observed around 250-290 nm. Absorbance within the 250-290 nm range is commonly attributed to π − π* electron transitions in aromatic and poly-aromatic compounds [22], which could connect with the impurities such as humic acid, phenol, DNA or proteins [23]. The decrease of the …”