Partial Characterization of a Virus Associated with Citrus Ringspot

Derrick, K. S.

doi:10.1094/phyto-78-1298

Cited by 60 publications

(47 citation statements)

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“…The B component of CtRSV-4 was partially purified from C. quinoa leaves bearing local lesions, as described by Derrick et al (1988). Preparations were treated with 0 .…”

Section: Purification Of Ctrsv Dsrna For Cloningmentioning

confidence: 99%

“…Partial characterization of various isolates in the USA (Derrick et al, 1988(Derrick et al, , 1991, Spain (NavasCastillo & Moreno, 1993, Israel (da Graça et al 1993), and Argentina (Garcia et al, 1991a,b) indicates that at least two sedimenting components (top or T and bottom or B) are required for infectivity, detected as local lesions in C. quinoa. T and B particles have similar morphology and consist of supercoiled circular filaments similar to those of Tenuiviruses (Garcia et al, 1994;Ramirez & Haenni, 1994;Milne et al, 1997) except that the contour length of B particles is much greater; T and B particles appear to share the same coat protein (Garcia et al, 1991b;da Graça et al, 1993).…”

Section: Introductionmentioning

confidence: 99%

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Detection of citrus psorosis‐ringspot virus using RT‐PCR and DAS‐ELISA

et al. 1997

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Psorosis, sometimes also associated with ringspot symptoms, is a widespread and damaging disease of citrus in many parts of the world including South America and the Mediterranean basin. We describe the application of RT‐PCR and DAS‐ELISA diagnostics to an isolate of citrus ringspot virus (CtRSV‐4) and other virus isolates associated with this disease. Fragments of cDNA from bottom‐component RNA of CtRSV‐4 were cloned and sequenced, and PCR primers were designed, 5′ACAATAAGCAAGACAAC upstream, and 5′CCATGTCACTTCTATTC downstream. RT‐PCR experiments using these primers allowed detection of CtRSV‐4 in infected citrus leaves down to a tissue dilution of 1/12 800 representing 2 μg of tissue, and less sensitive detection of the related citrus psorosis‐associated virus (CPsAV90‐1‐1) and four other psorosis isolates from Argentina and the USA. In addition, CtRSV‐4 particles were partially purified from local lesions in Chenopodium quinoa, and the preparations used to raise a rabbit antiserum. The antiserum was absorbed with extracts of healthy C. quinoa leaves, and a DAS‐ELISA kit was prepared and tested for detection of CtRSV‐4, CPsAV90–1‐1, and other psorosis isolates from Argentina, the USA, Italy and Spain. The ELISA detected CtRSV‐4 down to a tissue dilution of 1/1600, and most other psorosis isolates down to dilutions of 1/200–1/800. Three of a total of 20 heterologous isolates were consistently negative. Comparison of the PCR and ELISA results suggests that both methods can be used for detection of a range of psorosis isolates, but that variation of the viruses in the field might cause problems for any one diagnostic test.

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“…The B component of CtRSV-4 was partially purified from C. quinoa leaves bearing local lesions, as described by Derrick et al (1988). Preparations were treated with 0 .…”

Section: Purification Of Ctrsv Dsrna For Cloningmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Detection of citrus psorosis‐ringspot virus using RT‐PCR and DAS‐ELISA

et al. 1997

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“…Immunosorbent electron microscopy Antisera have been used to detect CPsV particles using immunosorbent electron microscopy (ISEM) [9,10,19,36,43]. ISEM technique was also applied for the efficient detection of CPsV in cross-protected citrus plants [33].…”

Section: Tissue Blot Immunoassaymentioning

confidence: 99%

“…CPsV virions are kinked filaments, 3-4 nm in diameter [19] that separate into two components during sucrose gradient centrifugation [9,18]. They consists of three single-stranded RNA (ssRNA) of negative polarity (vRNA) and a coat protein (CP) of * 48 kDa [5,58].…”

Section: Assessment Of the Biological Activitymentioning

confidence: 99%

Recent advances in Citrus psorosis virus

2014

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“…A partir do uso de clones nucelares, a sorose A, única forma descrita em nossas condições, deixou de ser problemática para a citricultura, embora surtos eventuais possam ocorrer, principalmente associados ao uso de borbulhas originadas de clones velhos. Dois componentes virais de morfologia diferenciada e com capa protéica, unidades de capsídeo de aproximadamente 48 KDa, têm sido associados a diferentes isolados de CtRSV e de sorose (Derrick et al, 1988;Garcia et al, 1994). Mais recentemente, considerando a similaridade entre partículas virais encontradas em diferentes fontes de sorose que causa forte descamação, estas foram descritas em um novo gênero Ophiovirus, como Citrus psorosis virus (CPsV) (Milne et al, 1996).…”

Section: Abstract Psorosis Virus Complex Elimination From Citrus By Sunclassified

Eliminação de vírus do complexo sorose dos citros por microenxertia associada a termoterapia

Carvalho¹,

Santos²,

Machado³

2002

Fitopatol. bras.

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A microenxertia de ápices caulinares tem sido utilizada com 100% de sucesso na eliminação do vírus da tristeza (Citrus tristeza virus) e dos viróides da exocorte (Citrus exocortis viroid - CEVd) e cachexia-xiloporose de materiais do Banco Ativo de Germoplasma de Citros do Centro de Citricultura Sylvio Moreira CCSM-IAC. Para o complexo da sorose, entretanto, esta técnica tem apresentado somente 60% de eficiência, indicando a necessidade de sua associação com termoterapia para garantir a eliminação viral. Para tanto, mudas originadas de borbulhas infetadas com sorose foram mantidas em câmara climática com 16 h de luz a 38 ºC e 8 h no escuro a 32 ºC e utilizadas para a obtenção dos ápices caulinares empregados na microenxertia. Após o pegamento, o conjunto micro porta-enxerto e brotação foi sobre-enxertado em limoeiro (Citrus limonia) 'Cravo' com sete meses de idade e mantido em condições de casa de vegetação. Clones de laranjeiras doces (Citrus sinsensis) 'Lima', 'Rubi', 'Piralima', 'Salustiana', 'João Nunes', 'Rosa' e 'Pêra Caire' tratados desta maneira, comprovaram eficiência de 100% de eliminação do complexo sorose, conforme indexação realizada empregando-se laranjeira 'Do Céu' como planta indicadora.

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Partial Characterization of a Virus Associated with Citrus Ringspot

Cited by 60 publications

References 0 publications

Detection of citrus psorosis‐ringspot virus using RT‐PCR and DAS‐ELISA

Detection of citrus psorosis‐ringspot virus using RT‐PCR and DAS‐ELISA

Recent advances in Citrus psorosis virus

Eliminação de vírus do complexo sorose dos citros por microenxertia associada a termoterapia

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