Parthenogenetic haploid embryonic stem cells efficiently support mouse generation by oocyte injection

Zhong, Cuiqing; Xie, Zhenfei; Yin, Qi; Dong, Rui; Yang, Suming; Wu, Yuxuan; Yang, Li; Li, Jinsong

doi:10.1038/cr.2015.132

Cited by 40 publications

(41 citation statements)

References 12 publications

(14 reference statements)

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“…Whether sperm RNAs are necessary for early embryonic development remains controversial 129–131 , particularly given the recent generation of parthenogenetic mice with appreciable survival rates 132,133 ; nonetheless, their potential synergistic action with maternal RNAs have been discussed with interest 134 . Thus far, the most significant biological functions identified for sperm RNAs are their involvement in non-Mendelian inheritance in mammals, such as the paramutation phenomenon in mice (BOX 2), and their contribution to the intergenerational inheritance of paternally acquired traits, including mental and nutritional stresses 9,24–26 .…”

Section: Figurementioning

confidence: 99%

Epigenetic inheritance of acquired traits through sperm RNAs and sperm RNA modifications

2016

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Once deemed heretical, emerging evidence now supports the notion that the inheritance of acquired characteristics can occur through ancestral exposures or experiences and that certain paternally acquired traits can be ‘memorized’ in the sperm as epigenetic information. The search for epigenetic factors in mammalian sperm that transmit acquired phenotypes has recently focused on RNAs and, more recently, RNA modifications. Here, we review insights that have been gained from studying sperm RNAs and RNA modifications, and their roles in influencing offspring phenotypes. We discuss the possible mechanisms by which sperm become acquisitive following environmental–somatic–germline interactions, and how they transmit paternally acquired phenotypes by shaping early embryonic development.

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Section: Figurementioning

confidence: 99%

Epigenetic inheritance of acquired traits through sperm RNAs and sperm RNA modifications

2016

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“…Surprisingly, when we attempted to identify the difference between PG-haESCs and AG-haESCs by performing RNA-seq analysis, we found that both cells showed high correlation based on all genes and all imprinted genes. Bisulphite-sequencing analysis of DMRs of typical imprinted genes indicated that maternal imprinted genes in PG-haESCs quickly lost the methylation imprint during the process of haESC derivation and exhibited a similar imprinted pattern to that of AG-haESCs [54]. Finally, we showed that PG-haESCs, after removal of H19 and Gtl2 DMRs, could gain the ability to produce live SC mice at an efficiency of 15% of transferred embryos [54].…”

Section: Potential Gamete Replacementmentioning

confidence: 67%

“…Recently, we further investigated the feasibility of PG‐haESCs for generation of SC mice via oocyte injection. We found that PG‐haESCs failed to support the full‐term development of the resulting SC embryos , probably because PG‐haESC‐derived SC embryos, consisting of two copies of female genomes, are actually PG diploid embryos that cannot develop to term in vivo . Surprisingly, when we attempted to identify the difference between PG‐haESCs and AG‐haESCs by performing RNA‐seq analysis, we found that both cells showed high correlation based on all genes and all imprinted genes.…”

Section: Characteristic Of Haescsmentioning

confidence: 89%

Generation and application of mammalian haploid embryonic stem cells

Bai

2016

J Intern Med

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“…; Zhong et al . ). In our previous study, the expression of imprinting genes in the blastocysts derived from two 8‐cell stage parthenogenetic embryo aggregations were better than that in the single parthenogenetic blastocysts (Shan et al .…”

Section: Discussionmentioning

confidence: 97%

“…Previous studies indicated that every embryo had a special imprint pattern, which was determined by the cell fate during development, the spatial origin of individual blastomeres affect the fate and spatial allocation of their progeny cells, especially in the first fate decision (Bruce & Zernicka-Goetz 2010). The ICM progenitor cells formed inner cells and outer cells during cell division at the 8-16-cell stage transition, the outer cells become TE and the inner cells become ICM reported that parthenogenetic haploid embryonic stem cells derived from a single oocyte contained only one set of the maternal genome, and the imprinting gene expression was different from each other Zhong et al 2016). In our previous study, the expression of imprinting genes in the blastocysts derived from two 8-cell stage parthenogenetic embryo aggregations were better than that in the single parthenogenetic blastocysts (Shan et al 2012).…”

Section: Discussionmentioning

confidence: 99%

Embryos aggregation improves development and imprinting gene expression in mouse parthenogenesis

et al. 2016

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Mouse parthenogenetic embryonic stem cells (PgESCs) could be applied to study imprinting genes and are used in cell therapy. Our previous study found that stem cells established by aggregation of two parthenogenetic embryos at 8-cell stage (named as a 2 PgESCs) had a higher efficiency than that of PgESCs, and the paternal expressed imprinting genes were observably upregulated. Therefore, we propose that increasing the number of parthenogenetic embryos in aggregation may improve the development of parthenogenetic mouse and imprinting gene expression of PgESCs. To verify this hypothesis, we aggregated four embryos together at the 4-cell stage and cultured to the blastocyst stage (named as 4aPgB). qPCR detection showed that the expression of imprinting genes Igf2, Mest, Snrpn, Igf2r, H19, Gtl2 in 4aPgB were more similar to that of fertilized blastocyst (named as fB) compared to 2aPgB (derived from two 4-cell stage parthenogenetic embryos aggregation) or PgB (single parthenogenetic blastocyst). Post-implantation development of 4aPgB extended to 11 days of gestation. The establishment efficiency of GFP-a 4 PgESCs which derived from GFP-4aPgB is 62.5%. Moreover, expression of imprinting genes Igf2, Mest, Snrpn, notably downregulated and approached the level of that in fertilized embryonic stem cells (fESCs). In addition, we acquired a 13.5-day fetus totally derived from GFP-a 4 PgESCs with germline contribution by 8-cell under zona pellucida (ZP) injection. In conclusion, four embryos aggregation improves parthenogenetic development, and compensates imprinting genes expression in PgESCs. It implied that a 4 PgESCs could serve as a better scientific model applied in translational medicine and imprinting gene study.

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Parthenogenetic haploid embryonic stem cells efficiently support mouse generation by oocyte injection

Cited by 40 publications

References 12 publications

Epigenetic inheritance of acquired traits through sperm RNAs and sperm RNA modifications

Epigenetic inheritance of acquired traits through sperm RNAs and sperm RNA modifications

Generation and application of mammalian haploid embryonic stem cells

Embryos aggregation improves development and imprinting gene expression in mouse parthenogenesis

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