Generation and application of mammalian haploid embryonic stem cells

Bai, Meizhu; Wu, Yuxuan; Li, J.

doi:10.1111/joim.12503

Cited by 22 publications

(13 citation statements)

References 57 publications

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“…Mammalian parthenogenetic embryos are now regarded as a less controversial source of ESCs, because of their inherent inability to form a new individual (Taupin, 2011;Bai et al, 2016). To date, PESCs have been successfully derived from several mammalian species including human, mouse, bovine, porcine, and rabbit (Bevacqua et al, 2011;De Sousa et al, 2016;Intawicha et al, 2016;Kim et al, 2016;Zhong et al, 2016).…”

Section: Application Of Pescsmentioning

confidence: 99%

Non‐coding RNAs: emerging regulatory factors in the derivation and differentiation of mammalian parthenogenetic embryonic stem cells

Cui

Xie

2017

Cell Biology International

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Parthenogenetic embryonic stem cells (PESCs) are ESCs derived from early parthenogenetic embryos. Haploid PESCs, containing haploid DNA, originate from a single sperm or occyte, while, diploid PESCs originate from two fused occytes. Most PESC lines used so far are diploid. PESCs exhibit representative pluripotent stem cell features, such as the capacity for self-renewal and the pariticular molecular signatures. Whereas, PESCs display distinctive properties, such as differential regulation of X-chromosome inactivation (XCI) and divergent monitor of genes involved in multiple biological processes. PESCs are considered promising in the regeneration medicine and developmental biology. Non-coding RNAs (ncRNAs), especially miRNAs and lncRNAs, have garnered increasing attention over the past 2 decades. They are now known to be involved in almost all cellular processes due to their full-range regulation of gene expression. Numerous studies have indicated that embryonic stem cells (ESCs) displayed distinct signatures of ncRNA genes, which play key roles in the pluripotency and self renewal of ESCs. However, the expression pattern of ncRNAs in PESCs and their roles in the derivation and differentiation of PESCs were rarely reported. In this paper, we reviewed recent research on the derivation and differentiation of PESCs and describe the emerging role of ncRNAs in these processes.

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Section: Application Of Pescsmentioning

confidence: 99%

Non‐coding RNAs: emerging regulatory factors in the derivation and differentiation of mammalian parthenogenetic embryonic stem cells

Cui

Xie

2017

Cell Biology International

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“…Remarkably, these ahESCs can produce viable and fertile progenies after intracytoplasmic injection into mature oocytes. The production of fertile adult mice bearing haESC-carried genetic traits further shows that the genetic information in haESCs is functionally complete and stable, which significantly enhances the merits of haploid stem cells as a new tool to quickly generate genetic models (Li et al, 2012;Yang et al, 2012;Bai et al, 2016).…”

Section: Introductionmentioning

confidence: 98%

Current advances in haploid stem cells

Cui

Zhou

et al. 2019

Protein Cell

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Diploidy is the typical genomic mode in all mammals. Haploid stem cells are artificial cell lines experimentally derived in vitro in the form of different types of stem cells, which combine the characteristics of haploidy with a broad developmental potential and open the possibility to uncover biological mysteries at a genomic scale. To date, a multitude of haploid stem cell types from mouse, rat, monkey and humans have been derived, as more are in development. They have been applied in high-throughput genetic screens and mammalian assisted reproduction. Here, we review the generation, unique properties and broad applications of these remarkable cells.

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“…Uniparental haploid embryos are efficient models for genome imprinting research and enable studies on the contribution of the paternal and maternal genome to early embryonic development. Moreover, haploid embryos have been used to derive embryonic stem cells and hold great promise for functional genetic studies and animal biotechnology (Panneerdoss et al, 2012; Kokubu and Takeda, 2014; Bai et al, 2016; Bai et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

Dysregulated gene expression of imprinted and X-linked genes: a link to poor development of bovine haploid androgenetic embryos

Águila

Therrien

Suzuki

et al. 2020

Preprint

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Mammalian uniparental embryos are efficient models for genome imprinting research and allow studies on the contribution of the paternal and maternal genome to early embryonic development. In this study, we analyzed different methodologies for production of bovine haploid androgenetic embryos (hAE) to elucidate the causes behind their poor developmental potential. The results showed that hAE can be efficiently generated by using intracytoplasmic sperm injection and oocyte enucleation at telophase II. Although haploidy does not disturb early development up to around the 3rd mitotic division, androgenetic development is disturbed after the time of zygote genome activation those that reach the morula stage are less capable to become a blastocyst. Analysis of gene expression indicated abnormal levels of methyltransferase 3B and key long non-coding RNAs involved in X-chromosome inactivation and genomic imprinting of the KCNQ1 locus, which is associated to the methylation status of imprinted control regions of XIST and KCNQ1OT1. Thus, our results seem to exclude micromanipulation consequences and chromosomal abnormalities as major factors in developmental restriction, suggesting that their early developmental constraint is regulated at an epigenetic level.

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Generation and application of mammalian haploid embryonic stem cells

Abstract: Content List -Read more articles from the symposium: Human models and technology for regenerative medicine.

Cited by 22 publications

References 57 publications

Non‐coding RNAs: emerging regulatory factors in the derivation and differentiation of mammalian parthenogenetic embryonic stem cells

Non‐coding RNAs: emerging regulatory factors in the derivation and differentiation of mammalian parthenogenetic embryonic stem cells

Current advances in haploid stem cells

Dysregulated gene expression of imprinted and X-linked genes: a link to poor development of bovine haploid androgenetic embryos

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