Parthenogenetic embryonic stem cells derived from cryopreserved newborn mouse ovaries: a new approach to autologous stem cell therapy

Xing, Fengying; Fang, Zhenfu; Qin, Bolin; Yao, Li; Hou, Jian; Chen, Xuejin

doi:10.1016/j.fertnstert.2008.01.054

Cited by 3 publications

(5 citation statements)

References 11 publications

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“…Similar to paAFSCs, paASCs were found to possess the expression of major pluripotency genes such as Oct4 and Nanog, but Sox2 had an apparently reduced expression in both cell lines. However, paASCs were found to have the capability to form teratomas and also contributed to E10.5 chimaeras, which confirmed their developmental pluripotency in vivo, consistent with previous studies [7,11,26,27]. Haploid embryonic stem cells from monogenetic gametes have been established as having a high expression of pluripotent genes and contributing to chimaera, though flow sorting every few passages for haploid cells is inevitable [28,29].…”

Section: Discussionsupporting

confidence: 88%

“…However, whether the low expression of Sox2 triggered the increased expression of Cdx2 and whether this gene expression pattern is related to imprinting genes in paAFSCs and paASCs require further exploration. Moreover, the expression of Sox2 in haploid stem cells is same as that in normal mouse ESCs [5,28,29,31], but no study of parthenogenetic diploid stem cells has shown the expression status of Sox2 [7,26,27]. Thus, whether the low expression of Sox2 is a common property of parthenogenetic diploid stem cells also needs further research.…”

Section: Discussionmentioning

confidence: 99%

“…Embryonic stem cells representative of naive (ESCs) and primed (EpiSCs) pluripotency have been established and converted to each other [23][24][25]. The parthenogenetic pluripotency ESCs (paESCs) has been derived from blastocysts stage; parthenogenetic EpiSCs (paEpiSCs) have only been generated post-implantation, though the culture conditions used for all paESCs and paEpiSCs included serum or feeder [7,9,26,27] and there have no reports on the conversion of paEpiSCs to paESCs. The findings of this study have revealed that paAFSCs in a primed state can be derived from blastocysts in a chemically defined medium, as well as that primed paAFSCs can be converted to naive paASCs.…”

Section: Discussionmentioning

confidence: 99%

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Derivation of Mouse Parthenogenetic Advanced Stem Cells

Wei

Zhang

Liu

et al. 2021

IJMS

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Parthenogenetic embryos have been widely studied as an effective tool related to paternal and maternal imprinting genes and reproductive problems for a long time. In this study, we established a parthenogenetic epiblast-like stem cell line through culturing parthenogenetic diploid blastocysts in a chemically defined medium containing activin A and bFGF named paAFSCs. The paAFSCs expressed pluripotent marker genes and germ-layer-related genes, as well as being alkaline-phosphatase-positive, which is similar to epiblast stem cells (EpiSCs). We previously showed that advanced embryonic stem cells (ASCs) represent hypermethylated naive pluripotent embryonic stem cells (ESCs). Here, we converted paAFSCs to ASCs by replacing bFGF with bone morphogenetic protein 4 (BMP4), CHIR99021, and leukemia inhibitory factor (LIF) in a culture medium, and we obtained parthenogenetic advanced stem cells (paASCs). The paASCs showed similar morphology with ESCs and also displayed a stronger developmental potential than paAFSCs in vivo by producing chimaeras. Our study demonstrates that maternal genes could support parthenogenetic EpiSCs derived from blastocysts and also have the potential to convert primed state paAFSCs to naive state paASCs.

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Section: Discussionsupporting

confidence: 88%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Derivation of Mouse Parthenogenetic Advanced Stem Cells

Wei

Zhang

Liu

et al. 2021

IJMS

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“…For example, mouse pESC lines that have been derived from cryopreserved ovaries, express ESC-specific markers and differentiate into embryoid bodies in vitro and teratomas in vivo. 123 Another study has also demonstrated the ability to produce parthenogenetic human blastocysts from cryopreserved oocytes at high efficiency rates. 124 Together these studies provide promise for cryopreserved eggs in the future as a potential source for pESCs.…”

Section: Parthenogenetic Stem Cellsmentioning

confidence: 99%

Current protocols in the generation of pluripotent stem cells: theoretical, methodological and clinical considerations

Swelstad

Kerr

2009

SCCAA

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Pluripotent stem cells have been derived from various embryonic, fetal and adult sources. Embryonic stem cells (ESCs) and parthenogenic ESCs (pESCs) are derived from the embryo proper while embryonic germ cells (EGCs), embryonal carcinoma cells (ECCs), and germ-line stem cells (GSC) are produced from germ cells. ECCs were the first pluripotent stem cell lines established from adult testicular tumors while EGCs are generated in vitro from primordial germ cells (PGCs) isolated in late embryonic development. More recently, studies have also demonstrated the ability to produce GSCs from adult germ cells, known as spermatogonial stem cells. Unlike ECCs, the source of GSCs are normal, non-cancerous adult tissue. The study of these unique cell lines has provided information that has led to the ability to reprogram somatic cells into an ESC-like state. These cells, called induced pluripotent stem cells (iPSCs), have been derived from a number of human fetal and adult origins. With the promises pluripotent stem cells bring to cell-based therapies there remain several considerations that need to be carefully studied prior to their clinical use. Many of these issues involve understanding key factors regulating their generation, including those which define pluripotency. In this regard, the following article discusses critical aspects of pluripotent stem cell derivation and current issues about their therapeutic potential.

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“…Mouse ES cells or their derivatives have been widely studied in the field of developmental biology, drug discovery and regenerative medicine [1][2][3] . Small molecules offer distinct advantages for the differentiation of mouse ES cells and are useful tools to facilitate therapeutic applications.…”

Section: Introductionmentioning

confidence: 99%

Promoting effects of isobavachin on neurogenesis of mouse embryonic stem cells were associated with protein prenylation

Wang

Kong

et al. 2011

Acta Pharmacol Sin

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Aim: Some small molecules can induce mouse embryonic stem (ES) cells to differentiate into neuronal cells. Here, we explored the effect of isobavachin (IBA), a compound with a prenyl group at position 8 of ring A, on promoting neuronal differentiation and the potential role of its protein prenylation. Methods: The hanging drop method was employed for embryonic body (EB) formation to mimic embryo development in vivo. The EBs were treated with IBA at a final concentration of 10 -7 mol/L from EB stage (d 4) to d 8+10. Geranylgeranyltransferase I inhibitor GGTI-298 was subsequently used to disrupt protein prenylation. Neuronal subtypes, including neurons and astrocytes, were observed by fluorescence microscopy. Gene and protein expression levels were detected using RT-PCR and Western blot analysis, respectively. Results: With IBA treatment, nestin was highly expressed in the neural progenitors generated from EBs (d 4, d 8+0). EBs then further differentiated into neurons (marked by β-tubulin III) and astrocytes (marked by GFAP), which were both up-regulated in a timedependent manner on d 8+5 and d 8+10. Co-treatment with GGTI-298 selectively abolished the IBA-induced neuronal differentiation. Moreover, in the MAPK pathway, p38 and JNK phosphorylation were down-regulated, while ERK phosphorylation was up-regulated after IBA treatment at different neuronal differentiation passages. Conclusion: IBA can facilitate mouse ES cells differentiating into neuronal cells. The mechanism involved protein prenylation and, subsequently, phos-ERK activation and the phos-p38 off pathway.

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Parthenogenetic embryonic stem cells derived from cryopreserved newborn mouse ovaries: a new approach to autologous stem cell therapy

Cited by 3 publications

References 11 publications

Derivation of Mouse Parthenogenetic Advanced Stem Cells

Derivation of Mouse Parthenogenetic Advanced Stem Cells

Current protocols in the generation of pluripotent stem cells: theoretical, methodological and clinical considerations

Promoting effects of isobavachin on neurogenesis of mouse embryonic stem cells were associated with protein prenylation

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