PARK7 and Nucleoside Diphosphate Kinase A as Plasma Markers for the Early Diagnosis of Stroke

Abstract

Background: Plasma markers for stroke could be useful in diagnosis and prognosis and in prediction of response of stroke patients to therapy. PARK7 and nucleoside diphosphate kinase A (NDKA) are increased in human postmortem cerebrospinal fluid (CSF), a model of global brain insult, suggesting that measurement in CSF and, more importantly, in plasma may be useful as a biomarker of stroke. Methods: We used ELISA to measure PARK7 and NDKA in plasma in 3 independent European and North American retrospective studies encompassing a total of 622 stroke patients and 165 control individuals. Results: Increases in both biomarkers were highly significant, with sensitivities of 54%-91% for PARK7 and 70%-90% for NDKA and specificities of 80%-97% for PARK7 and 90%-97% for NDKA. The concentrations of both biomarkers increased within 3 h of stroke onset. Conclusions: PARK7 and NDKA may be useful plasma biomarkers for the early diagnosis of stroke. In addition, this study demonstrated the utility of analysis of postmortem CSF proteins as a first step in the discovery of plasma markers of ischemic brain injury.

DJ-1 degrades transthyretin and an inactive form of DJ-1 is secreted in familial amyloidotic polyneuropathy

“…Furthermore, it has been reported that DJ-1 was secreted into the cerebrospinal fluid of patients with stroke and that DJ-1 may be used as a plasma marker for the early diagnosis of stroke (41). These findings together with our present study suggest that in addition to Parkinson's disease, DJ-1 is related to oxidative stress-related neurodegenerative disorders.…”

Proteomic Analysis of Cerebrospinal Fluid Changes Related to Postmortem Interval

“…Increases in nucleoside-diphosphate kinase 1 have been reported in blood (5 ), and increases in creatine kinase B have been reported in both blood (29 ) and CSF (30,31 ).…”

“…Almost all of these proteins were intracellular proteins, suggesting that they were released into the CSF upon cell death. In subsequent studies, those authors demonstrated that at least 2 of the proteins (nucleoside diphoshphate kinase A and posttranslationally modified prostaglandin D-2 synthase) have an altered serum concentration after stroke (5,6 ).…”

The Role of D-Dimer, Fibrinogen and C-Reactive Protein as Plasma Biomarkers in Acute Ischemic Stroke

“…Inflammation mediators such as CRP also had been investigated as markers for the diagnosis of stroke [3]. D-dimer, a marker of activation of the coagulation and fibrinolytic system has gained increasing interest over the recent years.…”