Parentage determination in maize hybrids using the arbitrarily primed polymerase chain reaction (AP-PCR)

Welsh, John; Honeycutt, Rhonda J.; McClelland, Michael; Sobral, Bruno W. S.

doi:10.1007/bf00588601

Cited by 159 publications

(60 citation statements)

References 11 publications

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“…Esta técnica tem se mostrado eficiente na identificação da variabilidade genética em diversos grupos de plantas e pode ser usada como uma ferramenta auxiliar em programas de melhoramento, encontrando também aplicação na obtenção de mapas genéticos (Williams et al, 1990;Reiter et al, 1992;Philipp et al, 1994;Paillard et al, 1996) e identificação de marcadores moleculares úteis na seleção assistida (Michelmore et al, 1991;Ohmore et al, 1995;Tartarini, 1996) entre outras. Marcadores RAPD foram usados para acessar a variabilidade genética contida em coleções de milho (Welsh et al, 1991), rosa (Gallego & Martinez, 1996), centeio (Huff, 1997), sorgo (Menkir et al, 1997) e arroz (Fuentes et al, 1999). Poucos exemplos do uso de RAPD têm sido mostrados na literatura em frutíferas.…”

Section: Introductionunclassified

Uso de marcadores moleculares na análise da variabilidade genética em acerola (Malpighia emarginata D.C.)

Salla

Ruas

et al. 2002

Rev. Bras. Frutic.

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RESUMO -A acerola (Malpighia emarginata) é uma frutífera tropical encontrada nativa na América Central e no Norte da América do Sul, sendo de grande importância econômica e social devido ao seu alto conteúdo de vitamina C (ácido ascórbico). Pomares de acerola têm sido preferencialmente estabelecidos por métodos de propagação vegetiva. No entanto, a propagação sexuada por sementes é igualmente utilizada e permite revelar um alto grau de polimorfismo na cultura, possibilitando a identificação de genótipos portadores de características de interesse agronômico. Vinte e quatro acessos de acerola, pertencentes ao Banco Ativo de Germoplasma da Universidade Estadual de Londrina, foram analisados, usando marcadores RAPD (Random amplified Polymorphic DNA) e obtidos com iniciadores (primers) de seqüência simples repetidas (SSRs). Um total de 164 e 73 marcadores foram obtidos com primers de RAPD e SSR, respectivamente. Os marcadores obtidos foram analisados, usando o método de agrupamentos UPGMA. A análise comparativa dos dendrogramas gerados com os primers de RAPD e com os primers SSR mostrou que, enquanto alguns acessos se associaram em grupos diferentes, outros apresentaram a mesma associação. Entretanto, maior polimorfismo entre acessos foi detectado com os primers de RAPD. A análise dos resultados revelou a alta variabilidade contida na coleção, permitindo associar o grau de similaridade genética, obtido por marcadores de DNA, com caracteres morfológicos compartilhados entre os acessos.Termos para indexação: Acerola, Malpighia emarginata, marcadores RAPD e SSR, variabilidade genética. THE USE OF MOLECULAR MARKERS IN THE GENETIC VARIABILITY ANALYSIS OF ACEROLA (Malphighia emarginata)ABSTRACT -Acerola (Malpighia emarginata) is a tropical fruit native from Central America and north of South America. It has shown an increasing economic and social importance due to its high vitamin C (ascorbic acid) content. Vegetative propagation is the preferable method used to establish acerola plantations. However, propagation by seeds has also been used allowing the identification and selection of genotypes that carry characteristics of agronomic interest. Twenty-four acerola accesses, of the Active Germplasm Bank of the Universidade Estadual de Londrina, were analyzed using molecular markers obtained with random amplified polymorphic DNA (RAPD) and simple sequence repeated (SSRs) primers. A total of 164 and 73 markers were obtained with RAPD and SSR primers, respectively. Polymorfic markers were scored as present or absent and analyzed using the UPGMA cluster analysis. The results presented reveal high levels of polymorphism in the studied collection. Comparative analysis of the phenograms, generated with both RAPD and SSR primers, revealed that while some accesses clustered in different groups other accesses presented the same association. However, there was large RAPD variation among the accesses. The associations observed with molecular markers were, for many accesses, the same of those determined on the basis of morphological char...

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Section: Introductionunclassified

Uso de marcadores moleculares na análise da variabilidade genética em acerola (Malpighia emarginata D.C.)

Salla

Ruas

et al. 2002

Rev. Bras. Frutic.

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“…Åkerman et al (1995) successfully used RAPDs in parentage confirmation of breeding progeny of European birch, but suggested that the information content of RAPD markers is not sufficient for identifying parents from many candidates, and co-dominant markers were recommended for such complex cases. In most cases, one cannot directly discriminate the dominant heterozygote from the dominant homozygote at RAPD loci, because of the dominant nature of these markers (Williams et al 1990, Carlson et al 1991, Welsh et al 1991. Therefore, it has been suggested that RAPDs would give less information than co-dominant markers for paternity analysis (Roy et al 1992).…”

Section: Introductionmentioning

confidence: 99%

Identification of the Male Parents of Half-sib Progeny from Japanese Black Pine (Pinus thunbergii Parl.) Clonal Seed Orchard using RAPD Markers.

2002

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Random amplified polymorphic DNA (RAPD) markers were used to identify the male parents of the half-sib progeny from a single clone within a Japanese black pine (Pinus thunbergii Parl.) clonal seed orchard consisting of 16 pinewood-nematode [Bursaphelenchus xylophilus (Steiner et Buhrer) Nickle] resistant clones. Twenty-one primers, with 28 polymorphic and reproducible fragments, were selected from 320 primers examined. The genotypes of orchard clones at each fragment were fingerprinted by RAPD analyses of both diploid needles and haploid tissues of megagametophytes from each clone. We used all 28 RAPD markers to identify the male parents of the half-sib progeny of a single orchard clone, Tanabe-(t)54. By comparing the genotype of orchard clones with the phenotype in the seedling at each RAPD locus, we were able to identify the male parents of 82 out of 85 seedlings. Pollen contamination and natural selfing occurred at rates of only 2.4 % and 1.2 %, respectively. Mating with the clone deviated significantly from panmixia. More than half of the progeny of the clone were produced through fertilization by only two clones. Seven clones did not contribute at all to fertilization as the male parent. Deviations from panmixia have been caused by a difference in the distance between clones, variation in the amounts of pollen, and phenological synchrony between clones. The possibility and limitations of RAPD markers for paternity analysis in conifer seed orchards are discussed.

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“…The applications of DNA markers include DNA ®ngerprinting for cultivar identi®cation and protection of plant variety rights, phylogenetic and diversity analysis, hybrid con®rmation, genome mapping and gene tagging for marker assisted selections. These marker assays especially, RAPD and ISSR-PCR markers have been shown to be useful in genetic ®ngerprinting of raspberry cultivars [9], maize [10], genetic diversity analysis in Brassica [11], grapevine [12], AzollaAnabaena [13], peanut [14], and celery cultivars [15]. These techniques have also been used for the genetic mapping of Capsicum species, pepper [16].…”

Section: Introductionmentioning

confidence: 99%

DNA profiling of disputed chilli samples (Capsicum annum) using ISSR-PCR and FISSR-PCR marker assays

Kumar

Kathirvel

Rao

et al. 2001

Forensic Science International

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A case of marketing of spurious seeds of chilli, Capsicum annum in the brand name of an elite variety referred to us from an Indian court of law, for identi®cation is described here. The highly reproducible molecular marker assays, inter simple sequence repeat polymerase chain reaction [ISSR-PCR] and FISSR-PCR (for¯uorescent ISSR-PCR) were used for differentiating the four disputed chilli samples. A total number of 17 ISSR anchored primers, which included nine di-, and eight tri-nucleotide primers were used for the analysis. The ISSR-PCR products were separated on a 2% agarose gel. A total of 212 and 288 bands were resolved by seven di-and eight tri-nucleotide primers, respectively, with an average of 30 bands per primer. Five out of nine dinucleotide primers and four out of eight trinucleotide primers could unambiguously differentiate all the four disputed chilli samples. The sensitivity and informativeness of the ISSR-PCR assay were further enhanced by the use of FISSR-PCR technique. The FISSR-PCR assay revealed a total number of 566 bands using three tri-and one di-nucleotide primers with an average of 141 bands per primer. These four primers could reliably distinguish all the four disputed samples unambiguously. In developing countries like India, violation of Plant Breeder's Rights is a major concern of law. The present report is, therefore, a step to protect the Plant Breeder's Rights by making use of reliable and modern DNA technologies. #

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Parentage determination in maize hybrids using the arbitrarily primed polymerase chain reaction (AP-PCR)

Cited by 159 publications

References 11 publications

Uso de marcadores moleculares na análise da variabilidade genética em acerola (Malpighia emarginata D.C.)

Uso de marcadores moleculares na análise da variabilidade genética em acerola (Malpighia emarginata D.C.)

Identification of the Male Parents of Half-sib Progeny from Japanese Black Pine (Pinus thunbergii Parl.) Clonal Seed Orchard using RAPD Markers.

DNA profiling of disputed chilli samples (Capsicum annum) using ISSR-PCR and FISSR-PCR marker assays

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