Parenchymal pressure inconsistency in different brain areas after kaolin injection into subarachnoid space of neonatal rats

Vural, Murat; Cosan, T. Erhan; Uzuner, Kubi̇lay; Erkasap, Nilüfer; Coşan, Didem Turgut; Bal, Cengiz

doi:10.5137/1019-5149.jtn.18072-16.1

Cited by 1 publication

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References 19 publications

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“…Kaolin is known to induce inflammation and foreign body reactions and has been used to induce adhesions in animal models, especially in pulmonary fibrosis (7), hepatic fibrosis (8), and subarachnoid dural adhesion clinical models (9). This study tested the dose-dependent effects of kaolin to induce adhesions in a rat colon abrasion model.…”

Section: Introductionmentioning

confidence: 99%

A Novel Rat Model to Test Intra-Abdominal Anti-adhesive Therapy

et al. 2020

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Background: Adhesion formation after abdominal surgery is considered almost inevitable and a major cause of morbidity. Novel treatments have been proposed, however there is a lack of suitable small animal models for pre-clinical evaluation, mainly due to inconsistency in adhesion formation in positive control animals. Here, we propose a new rat model of abdominal adhesions using Kaolin as the adhesion-inducing agent at an optimized dosage for testing newer agents in respect to their anti-adhesive property.Materials and Methods: Twenty-five adult (8-10 week old) male Wistar albino rats underwent midline laparotomy and caecal abrasion and were randomized to receive topical applications of normal saline or different concentrations and volumes of a Kaolin-based formulation. At day 14 rats were humanely killed, and adhesions graded macroscopically by an investigator blinded to the treatment groups, using pre-determined adhesion scores and microscopically using histopathology.Results: Kaolin at 0.005 g/mL caused consistent adhesions without compromising rat viability. At higher doses significant morbidity and mortality was observed in the animals treated.Conclusions: Kaolin induced adhesion in a rat abdominal surgery model is reliable and can be safely used to test the efficacy of novel anti-adhesive formulations to prevent intra-abdominal adhesions.

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Section: Introductionmentioning

confidence: 99%