A Novel Rat Model to Test Intra-Abdominal Anti-adhesive Therapy

Sundaresan, Rajan; Bennett, Catherine; Bassiouni, Ahmed; Smith, Matthew H.; Finnie, John; Trochsler, Markus; Psaltis, Alkis James; Vreugde, Sarah; Wormald, Peter John

doi:10.3389/fsurg.2020.00012

Cited by 6 publications

(6 citation statements)

References 18 publications

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“…As a next step, our goal was to quantify the extent of adhesion formation. Rats were euthanized at endpoint (POD 42), and the surgical sites were excised for further macroscopic observations, based on previous findings regarding formation of adhesions ( Rodgers and diZerega, 1993 ; Chegini et al, 2002 ; Ergul and Korukluoglu, 2008 ; Maciver et al, 2011 ; Coccolini et al, 2013 ; Fang et al, 2018 ; Vediappan et al, 2020 ) after tissue trauma. We performed a macroscopic assessment of adhesion formation and envisioned a scoring system raging from 0 to 3 ( Figure 5 ).…”

Section: Resultsmentioning

confidence: 99%

Bioglass/ceria nanoparticle hybrids for the treatment of seroma: a comparative long-term study in rats

Pais,

Papanikolaou,

Hoyos

et al. 2024

Front. Bioeng. Biotechnol.

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Background: Seroma formation is a common postoperative complication. Fibrin-based glues are typically employed in an attempt to seal the cavity. Recently, the first nanoparticle (NP)-based treatment approaches have emerged. Nanoparticle dispersions can be used as tissue glues, capitalizing on a phenomenon known as ‘nanobridging’. In this process, macromolecules such as proteins physically adsorb onto the NP surface, leading to macroscopic adhesion. Although significant early seroma reduction has been shown, little is known about long-term efficacy of NPs. The aim of this study was to assess the long-term effects of NPs in reducing seroma formation, and to understand their underlying mechanism.Methods: Seroma was surgically induced bilaterally in 20 Lewis rats. On postoperative day (POD) 7, seromas were aspirated on both sides. In 10 rats, one side was treated with NPs, while the contralateral side received only NP carrier solution. In the other 10 rats, one side was treated with fibrin glue, while the other was left untreated. Seroma fluid, blood and tissue samples were obtained at defined time points. Biochemical, histopathological and immunohistochemical assessments were made.Results: NP-treated sides showed no macroscopically visible seroma formation after application on POD 7, in stark contrast to the fibrin-treated sides, where 60% of the rats had seromas on POD 14, and 50% on POD 21. At the endpoint (POD 42), sides treated with nanoparticles (NPs) exhibited significant macroscopic differences compared to other groups, including the absence of a cavity, and increased fibrous adhesions. Histologically, there were more macrophage groupings and collagen type 1 (COL1) deposits in the superficial capsule on NP-treated sides.Conclusion: NPs not only significantly reduced early manifestations of seroma and demonstrated an anti-inflammatory response, but they also led to increased adhesion formation over the long term, suggesting a decreased risk of seroma recurrence. These findings highlight both the adhesive properties of NPs and their potential for clinical therapy.

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Section: Resultsmentioning

confidence: 99%

Bioglass/ceria nanoparticle hybrids for the treatment of seroma: a comparative long-term study in rats

Pais,

Papanikolaou,

Hoyos

et al. 2024

Front. Bioeng. Biotechnol.

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“…Admittedly, among the limitations of the currently employed model is that this study looks at a solitary point in time for determining the adhesion promotion or inhibition of these products, and two weeks were chosen for the evaluation of adhesion during an early postoperative period which might not correlate with the degree of adhesion formation that proceeds after longer intervals. However, this appears to be a standard timeframe for evaluating adhesions in animal care and the limitations of the model we used [ 53 ]. Therefore, further studies are demanded to investigate the influence of colchicine, the combination of (MP + DPH) and prednisolone on adhesion formation at later stages associated with clinical practice.…”

Section: Discussionmentioning

confidence: 99%

Comparison of the preventive effect of colchicine versus diphenhydramine, prednisolone, and a combination therapy on intraperitoneal adhesion bands: an experimental study in rats

et al. 2023

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Background Peritoneal adhesion formation is an inevitable consequence of abnormal repair of the peritoneum following different peritoneal injuries of intra-abdominal operations with the subsequent morbidity that they represent. Vast efforts have been made to elucidate the cause and prevent the development of abdominal adhesions. The aim of our study is to compare the capability of colchicine versus diphenhydramine (DPH) and methylprednisolone (MP), and also prednisolone in adhesion prevention. Methods Sixty-one male Wistar stock rats were divided into four groups. The first group attended as the control group. Groups 2, 3, and 4 received oral combination of MP + DPH solution (20 mg/kg), colchicine (0.02 mg/kg), and prednisolone (1 mg/ kg), respectively. Adhesion bands were induced by standardized abrasion of the peritoneum through a midline laparotomy. All rats were sacrificed on the 15th-day post medication administration and the subjects underwent an exploratory laparotomy. The presence of adhesions was evaluated with the modified using Nair's classification. Results The proportion of the control group with substantial adhesion bands (73.3%) was significantly higher than that of the MP + DPH (13.3%), colchicine (33.3%), and prednisolone (31.3%) groups. There were significant differences between the scores of the control and the MP + DPH, colchicine, and prednisolone groups (P = 0.001, 0.028, and 0.019, respectively). There was no statistically significant difference to favor colchicine against MP + DPH (P = 0.390) or MP + DPH against prednisolone (P = 0.394). Conclusions Both colchicine and combination of DPH + MP prevented postoperative abdominal adhesions separately in our study. However, the lowest adhesion formation rate was observed in the DPH + MP group, even lower than the prednisolone group.

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“…Rats underwent a laparotomy and a colon abrasion [ 16 ] or a colon abrasion with enterotomy [ 21 ]. Briefly, the abdomen was shaved and prepared with alcohol.…”

Section: Methodsmentioning

confidence: 99%

“…The caecum was delivered and kept moist with saline-soaked gauze whilst a dry gauze was used to rub the caecum repeatedly until sub-serosal bleeding occurred over an area of 1 cm 2 ( Fig 1B & 1C ). 2 ml 0.005 g/mL Kaolin in saline was instilled over the abrasion [ 21 ]. The caecum was then returned to the abdomen and the abdominal wall closed in layers with a 3–0 Polyglactin suture.…”

Section: Methodsmentioning

confidence: 99%

Prevention of adhesions post-abdominal surgery: Assessing the safety and efficacy of Chitogel with Deferiprone in a rat model

et al. 2021

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Introduction Adhesions are often considered to be an inevitable consequence of abdominal and pelvic surgery, jeopardizing the medium and long-term success of these procedures. Numerous strategies have been tested to reduce adhesion formation, however, to date, no surgical or medical therapeutic approaches have been successful in its prevention. This study demonstrates the safety and efficacy of Chitogel with Deferiprone and/or antibacterial Gallium Protoporphyrin in different concentrations in preventing adhesion formation after abdominal surgery. Materials and methods 112 adult (8–10 week old) male Wistar albino rats were subjected to midline laparotomy and caecal abrasion, with 48 rats having an additional enterotomy and suturing. Kaolin (0.005g/ml) was applied to further accelerate adhesion formation. The abrasion model rats were randomized to receive saline, Chitogel, or Chitogel plus Deferiprone (5, 10 or 20 mM), together with Gallium Protoporphyrin (250μg/mL). The abrasion with enterotomy rats were randomised to receive saline, Chitogel or Chitogel with Deferiprone (1 or 5 mM). At day 21, rats were euthanised, and adhesions graded macroscopically and microscopically; the tensile strength of the repaired caecum was determined by an investigator blinded to the treatment groups. Results Chitogel with Deferiprone 5 mM significantly reduced adhesion formation (p<0.01) when pathologically assessed in a rat abrasion model. Chitogel with Deferiprone 5 mM and 1 mM also significantly reduced adhesions (p<0.05) after abrasion with enterotomy. Def-Chitogel 1mM treatment did not weaken the enterotomy site with treated sites having significantly better tensile strength compared to control saline treated enterotomy rats. Conclusions Chitogel with Deferiprone 1 mM constitutes an effective preventative anti-adhesion barrier after abdominal surgery in a rat model. Moreover, this therapeutic combination of agents is safe and does not weaken the healing of the sutured enterotomy site.

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A Novel Rat Model to Test Intra-Abdominal Anti-adhesive Therapy

Cited by 6 publications

References 18 publications

Bioglass/ceria nanoparticle hybrids for the treatment of seroma: a comparative long-term study in rats

Bioglass/ceria nanoparticle hybrids for the treatment of seroma: a comparative long-term study in rats

Comparison of the preventive effect of colchicine versus diphenhydramine, prednisolone, and a combination therapy on intraperitoneal adhesion bands: an experimental study in rats

Prevention of adhesions post-abdominal surgery: Assessing the safety and efficacy of Chitogel with Deferiprone in a rat model

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