Parathyroid hormone-related peptide is involved in protection against invasion of tooth germs by bone via promoting the differentiation of osteoclasts during tooth development

Liu, Ji Guang; Tabata, M.; Fujii, Takafumi; Ohmori, Tomoharu; Abe, Makoto; Ohsaki, Yasuyoshi; Kato, Jun; Wakisaka, Satoshi; Iwamoto, Masahiro; Kurisu, Kojiro

doi:10.1016/s0925-4773(00)00366-x

Cited by 30 publications

(13 citation statements)

References 42 publications

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“…Therefore, we have used the antisense technique and examined cultured tooth germs of incisors to analyze the function of dHAND in incisor development. The antisense technique has been shown to be an effective method for investigating the function of molecules involved in tooth development, including epidermal gowth factor (Kronmiller et al 1991;Shum et al 1993), amelogenin (Diekwisch et al 1993), transforming growth factor-2 (Chai et al 1994), hepatocyte growth factor (Tabata et al 1996), and parathyroid hormone-related peptide (Liu et al 2000). Using an in ovo culture system, Srivastava et al (1995) have shown that treatment with antisense ODNs against dHAND and eHAND simultaneously affect heart looping.…”

Section: Discussionmentioning

confidence: 99%

“…Organ culture of incisor tooth germs of mice Explants containing incisor tooth germs of the lower incisor were obtained from mandibles of E14.5 mice and cultured in a modified Trowell's system for periods of up to 11 days as described elsewhere (Tabata et al 1996;Liu et al 2000). Explants were cultured in BGJb medium (Fitton-Jackson's modified BGJ; Gibco BRL, Gaithersburg, USA), containing 100 µg/ml ascorbic acid (Nakai Chemical, Kyoto, Japan) and 100 U/ml penicillin-streptomycin (Gibco BRL).…”

Section: Animalsmentioning

confidence: 99%

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Tooth-type specific expression of dHAND/Hand2: possible involvement in murine lower incisor morphogenesis

Abe

Tamamura

Yamagishi

et al. 2002

Cell and Tissue Research

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dHAND/Hand2 is a basic helix-loop-helix transcription factor required for the development of the heart, pharyngeal arches, and vasculature and is expressed during embryogenesis. However, there are no reports on the involvement of the dHAND gene in tooth development. In the present study, the expression of dHAND was examined in developing tooth germs of mice. The dHAND gene was expressed in the mesenchyme of the presumptive incisor region of the lower jaw at an early stage and in the mesenchyme of the lower incisor tooth germ at a later stage. However, the dHAND gene was not expressed in the upper incisor region or the upper and lower molar regions during jaw development. Treatment of tooth germ explants of lower incisors with antisense oligodeoxinucleotide (ODN) against dHAND prevented the differentiation of tooth germ cells, including ameloblasts and odontoblasts, the formation of dentin and enamel, and the proliferation of tooth germ cells and increased the apoptosis of tooth germ cells, suggesting that dHAND is essential for these cells during development. On the other hand, the treatment of tooth germ explants of upper incisor and upper or lower molars did not induce severe effects on their development. Treatment of the explants with basic fibroblast growth factor in association with antisense ODN partially rescued them from the effects of antisense ODN. The present results suggest that the dHAND gene plays important roles in type-specific development of lower incisors, and that basic fibroblast growth factor is involved downstream of the dHAND pathway in tooth germ cells.

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Section: Discussionmentioning

confidence: 99%

Section: Animalsmentioning

confidence: 99%

Tooth-type specific expression of dHAND/Hand2: possible involvement in murine lower incisor morphogenesis

Abe

Tamamura

Yamagishi

et al. 2002

Cell and Tissue Research

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“…In humans, abnormal crown patterns in patients with congenital syphilis have been ascribed to result from infection of the tooth follicle (3). Likewise, loss of function of parathyroid hormone-related protein (PTHrP) leads to loss of alveolar bone resorption and deformation of the tooth crown in the mouse (15)(16)(17)(18)(19). A lethal human syndrome called Blomstrand chondrodysplasia, which involves a loss of function of the receptor for PTHrP, results in a severe deformation of the base of a tooth crown due to an excess of bone formation (20).…”

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confidence: 99%

Mechanical constraint from growing jaw facilitates mammalian dental diversity

Renvoisé

Kavanagh

Lazzari

et al. 2017

Proc. Natl. Acad. Sci. U.S.A.

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Much of the basic information about individual organ development comes from studies using model species. Whereas conservation of gene regulatory networks across higher taxa supports generalizations made from a limited number of species, generality of mechanistic inferences remains to be tested in tissue culture systems. Here, using mammalian tooth explants cultured in isolation, we investigate self-regulation of patterning by comparing developing molars of the mouse, the model species of mammalian research, and the bank vole. A distinct patterning difference between the vole and the mouse molars is the alternate cusp offset present in the vole. Analyses of both species using 3D reconstructions of developing molars and jaws, computational modeling of cusp patterning, and tooth explants cultured with small braces show that correct cusp offset requires constraints on the lateral expansion of the developing tooth. Vole molars cultured without the braces lose their cusp offset, and mouse molars cultured with the braces develop a cusp offset. Our results suggest that cusp offset, which changes frequently in mammalian evolution, is more dependent on the 3D support of the developing jaw than other aspects of tooth shape. This jaw-tooth integration of a specific aspect of the tooth phenotype indicates that organs may outsource specific aspects of their morphology to be regulated by adjacent body parts or organs. Comparative studies of morphologically different species are needed to infer the principles of organogenesis.T he conservation of gene regulatory networks in the development of homologous organs is well exemplified by dentitions. Teeth in fish, reptiles, and mammals have been found to use largely shared developmental gene networks (1, 2). Furthermore, tooth development, similar to organ development in general, results from sequential events that appear to be relatively independent from the development of other organs. In particular, mammalian teeth have been considered highly selfregulatory, as they form inside the dental follicle buffered from the surrounding environment (3-5). The self-regulatory aspect of teeth is further supported by the reiterative use of the same signaling pathways and signaling centers, called enamel knots, present within each growing tooth (2). Experiments on growing teeth have revealed how an activator-inhibitor balance of signaling molecules within a developing tooth and between adjacent teeth regulate dental patterning (6-12).Despite the evidence from mice indicating self-regulation of teeth, several observations suggest an intriguing link between jaw bone and tooth crown formation (3,13,14). In humans, abnormal crown patterns in patients with congenital syphilis have been ascribed to result from infection of the tooth follicle (3). Likewise, loss of function of parathyroid hormone-related protein (PTHrP) leads to loss of alveolar bone resorption and deformation of the tooth crown in the mouse (15)(16)(17)(18)(19). A lethal human syndrome called Blomstrand chondrodysplasia, which invol...

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“…Cultured explants were fixed with 4% paraformaldehyde in a 0.1 M cacodylate buffer (pH 7.3) at 4 for 30 min -overnight, dehydrated through up-graded alcohols, deposited (Tabata et al, 1996a(Tabata et al, , 2002Liu et al, 2000;Shibaguchi et al, 2003).…”

Section: Light Microscopymentioning

confidence: 99%

Introduction of a three-dimensional and layered (TDL) culture, a novel primary co-culture method for ameloblasts and pulp-derived cells

Notani

Tabata

Iseki

et al. 2009

Archives of Histology and Cytology

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polarity and well-developed intercellular junctions, had PAS positive material in their cytoplasm, and expressed a distinct immunoreactivity for cyotokeratin 14 and amelogenins. Pulpal cells in the gel displayed a strong ALP activity throughout the 3D gel. The current observations have clearly shown that the structural and functional features reminiscent of early secretory ameloblasts could be restored in the enamel organ-derived cells in a TDL culture.

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Parathyroid hormone-related peptide is involved in protection against invasion of tooth germs by bone via promoting the differentiation of osteoclasts during tooth development

Cited by 30 publications

References 42 publications

Tooth-type specific expression of dHAND/Hand2: possible involvement in murine lower incisor morphogenesis

Tooth-type specific expression of dHAND/Hand2: possible involvement in murine lower incisor morphogenesis

Mechanical constraint from growing jaw facilitates mammalian dental diversity

Introduction of a three-dimensional and layered (TDL) culture, a novel primary co-culture method for ameloblasts and pulp-derived cells

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