Introduction of a three-dimensional and layered (TDL) culture, a novel primary co-culture method for ameloblasts and pulp-derived cells

Abstract

polarity and well-developed intercellular junctions, had PAS positive material in their cytoplasm, and expressed a distinct immunoreactivity for cyotokeratin 14 and amelogenins. Pulpal cells in the gel displayed a strong ALP activity throughout the 3D gel. The current observations have clearly shown that the structural and functional features reminiscent of early secretory ameloblasts could be restored in the enamel organ-derived cells in a TDL culture.

Epithelial Cell Coculture Models for Studying Infectious Diseases: Benefits and Limitations

“…Using this architecture, the proximity of different cell types can be assayed for morphological changes to cell structure, or migration of cells [23]. In dentistry, the study of early tooth germ cell structure has been observed through the use of 3D and layered cultures that utilise dental tooth pulp and enamel epithelial cells suspended in layers that are then floated in media [68]. This resulted in a cuboidal morphology of enamel epithelial cells, which correlates with developmental effects in vivo.…”

“…This resulted in a cuboidal morphology of enamel epithelial cells, which correlates with developmental effects in vivo. Here, the result would have been a simple monolayer if the epithelial cells were studied in monoculture [68]. 3D matrix gels have also demonstrated migration and differentiation when cocultured in layers.…”