“…4 However, information on the development of the thresholds and effects of different numbers of larvae is sparse. 32,33,[66][67][68] Our results show that there was no significant yield effect when plants were infested with larvae at GS11-12 at current European action thresholds of five larvae or lower. However, introducing high numbers of larvae (i.e., 25, but with a projected mean infestation rate of 6.5 larvae per plant with a range between 5 and 8), did significantly reduce plant height, flower and pod production, the number of seeds produced, and their quality (lower oil content and higher concentrations of glucosinolate), suggesting that the physiological injury level, the level of injury where a plant begins to ensure yield penalties, 69 for P. chrysocephala larvae is between five and 25 larvae/plant in this experimental set up.…”