ParaSight™-F test results in cerebral malaria patients before and after treatment in Chittagong Medical College Hospital, Bangladesh

Faiz, Abul; Rashid, Rehnuma; Palit, Rajib; Rahman, Ridwanur; BinYunus, Emran; Hussain, Amir; Rahman, Ekhlasur; Talukdar, Krishna; Bangali, A Mannan; Montanari, Richard

doi:10.1016/s0035-9203(00)90439-5

Cited by 10 publications

(5 citation statements)

References 3 publications

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“…In particular, test methods based on HRP-2 antigen capture have been reported to detect persistent peripheral antigenemia for upwards of 28 days after cure of P. falciparum (12,14) and in aparasitemic women with placental malaria (21). The consequence of persistent assay positivity for clinical utility and patient management remains to be determined, but it is not likely to be a major factor in the diagnosis of malaria in nonimmune travelers or other populations with limited exposure, such as deployed military forces.…”

Section: Discussionmentioning

confidence: 99%

Malaria Rapid Diagnostic Devices: Performance Characteristics of the Para Sight F Device Determined in a Multisite Field Study

et al. 2001

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Microscopic detection of parasites has been the reference standard for malaria diagnosis for decades. However, difficulty in maintaining required technical skills and infrastructure has spurred the development of several nonmicroscopic malaria rapid diagnostic devices based on the detection of malaria parasite antigen in whole blood. The ParaSight F test is one such device. It detects the presence of Plasmodium falciparum-specific histidine-rich protein 2 by using an antigen-capture immunochromatographic strip format. The present study was conducted at outpatient malaria clinics in Iquitos, Peru, and Maesod, Thailand. Duplicate, blinded, expert microscopy was employed as the reference standard for evaluating device performance. Of 2,988 eligible patients, microscopy showed that 547 (18%) had P. falciparum, 658 (22%) had P. vivax, 2 (0.07%) had P. malariae, and 1,750 (59%) were negative for Plasmodium. Mixed infections (P. falciparum and P. vivax) were identified in 31 patients (1%). The overall sensitivity of ParaSight F for P. falciparum was 95%. When stratified by magnitude of parasitemia (no. of asexual parasites per microliter of whole blood), sensitivities were 83% (>0 to 500 parasites/l), 87% (501 to 1,000/l), 98% (1,001 to 5,000/l), and 98% (>5,000/l). Device specificity was 86%.

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Section: Discussionmentioning

confidence: 99%

Malaria Rapid Diagnostic Devices: Performance Characteristics of the Para Sight F Device Determined in a Multisite Field Study

et al. 2001

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“…2 There are only limited data on posttreatment persistence of HRP2 antigenemia with the immunoglobulin (Ig) M monoclonal antibody to HRP2 used in the ICT Pf 6 and Pf/Pv tests, 16,18,20 but these suggest rates of persistence at least as high as those found with the IgG HRP2 monoclonal antibody used in the ParaSight-F test. [3][4][5][7][8][9][10]13,14 In the Sumbanese population described above, we found persistence of HRP2 in 29 and 10% of those negative for asexual parasites by microscopy on Days 7 and 14, respectively. 20 The causes of posttreatment persistence of HRP2 with both the ICT test and ParaSight-F tests are unclear; they may vary with drug treatment used and geographic region.…”

Section: Discussionmentioning

confidence: 53%

“…2 However, there are only limited data on the ability of antigen detection tests to detect and predict TF when used during or after the completion of therapy. Histidine rich protein 2 (HRP2) antigen is known to persist after resolution of clinical symptoms and clearance of viable asexual parasitemia, [3][4][5][6][7][8][9][10][11][12][13][14] which may limit its ability to reliably predict recrudescent parasitemia. Although some studies have suggested rapid antigen detection tests may be potentially useful for monitoring the response to antimalarial therapy or predicting TF, [7][8][9]12,15 concern has been raised by other studies that persistence of HRP2 antigen after clearance of parasitemia may lead to confusing results when used for this purpose.…”

Section: Introductionmentioning

confidence: 99%

“…Although some studies have suggested rapid antigen detection tests may be potentially useful for monitoring the response to antimalarial therapy or predicting TF, [7][8][9]12,15 concern has been raised by other studies that persistence of HRP2 antigen after clearance of parasitemia may lead to confusing results when used for this purpose. 2,14,16 Moreover, none of the studies that have addressed this issue [7][8][9]12,[14][15][16] have used the 1997 World Health Organization (WHO) protocol for assessment of the therapeutic efficacy of antimalarial drugs. 17 The ICT Malaria Pf/Pv test is based on the immunochromatographic detection of the P. falciparum-specific HRP2 antigen and a panmalarial antigen found in asexual stages of P. falciparum and P. vivax and sexual stages of P. falciparum.…”

Section: Introductionmentioning

confidence: 99%

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Detection of histidine rich protein 2 and panmalarial ICT Malaria Pf/Pv test antigens after chloroquine treatment of uncomplicated falciparum malaria does not reliably predict treatment outcome in eastern Indonesia.

Tjitra

Suprianto

Dyer³

et al. 2001

The American Journal of Tropical Medicine and Hygiene

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Abstract. In regions with drug-resistant malaria, the ability to rapidly detect or predict treatment failure (TF) soon after a course of standard therapy for Plasmodium falciparum malaria would facilitate the prompt institution of secondline therapy. We thus evaluated longitudinally the ability of the ICT Malaria Pf/Pv immunochromatographic test to predict treatment outcome. Sixty-six Sumbanese Indonesians with uncomplicated falciparum malaria were treated with chloroquine and followed for 28 days by use of 1997 World Health Organization criteria for assessment of therapeutic efficacy of antimalarial drugs. The ICT Pf/Pv testing could be compared with microscopy in approximately half of the patients on each day of follow-up. Although strongly positive histidine rich protein 2 (HRP2) line intensities (equal to or greater than the control band) in convalescence were highly predictive of TF, any degree of positivity for the HRP2 and panmalarial antigens in convalescence was only moderately predictive of TF. Positive predictive values of the HRP2 and panmalarial antigens for TF were 76.9% and 87.0%, respectively, on Day 3, 82.4% and 87.5% on Day 7, and 78.9% and 78.9% on Day 14. Negative HRP2 and panmalarial antigen results in convalescence were even less predictive of an adequate clinical response, and false-negative HRP2 and panmalarial antigen test results were found in one-sixth (6 of 37) of recrudescent infections diagnosed by microscopy among patients with late treatment failure. To reliably predict treatment outcome with rapid antigen tests, further development appears necessary to improve sensitivity for viable asexual parasites while avoiding detection of both gametocytes and persistent antigen in convalescence.

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“…3 Alternative immunochromatography tests have been developed, particularly for Plasmodium falciparum that showed to be effective, quick and easy to use. 1 Subjects seen at the Evandro Chagas Institute, Belém, from January to March 2000 with symptoms and signs suggestive of malaria and later had a positive slide test for P. vivax malaria were randomly tested using ICT Pf/Pv TM . Informed consent was obtained and symptoms details were registered.…”

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confidence: 99%

Performance of an immunochromatography test for vivax malaria in the Amazon region, Brazil

et al. 2003

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The study was carried out to evaluate the diagnostic performance of the ICT malaria Pf/Pv TM test for vivax malaria diagnosis in Belém, Amazon region, Brazil. The results of blood malaria parasites examination using an immunochromatography test were compared with thick blood film (TBF) examination. It was also evaluated the performance of this test storaged at three different temperatures (25°C, 30°C, and 37°C) for 24 hours before use. Overall sensitivity of ICT Pf/Pv TM was 61.8% with a specificity of 100%, positive and negative predictive value of 100% and 71.8%, respectively and accuracy of 80.6%. The test sensitivity was independent of the parasite density. This test needs to be further reviewed in order to have better performance for P. vivax malaria diagnosis. Resumo

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ParaSight™-F test results in cerebral malaria patients before and after treatment in Chittagong Medical College Hospital, Bangladesh

Cited by 10 publications

References 3 publications

Malaria Rapid Diagnostic Devices: Performance Characteristics of the Para Sight F Device Determined in a Multisite Field Study

Malaria Rapid Diagnostic Devices: Performance Characteristics of the Para Sight F Device Determined in a Multisite Field Study

Detection of histidine rich protein 2 and panmalarial ICT Malaria Pf/Pv test antigens after chloroquine treatment of uncomplicated falciparum malaria does not reliably predict treatment outcome in eastern Indonesia.

Performance of an immunochromatography test for vivax malaria in the Amazon region, Brazil

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