Parasediminibacterium paludis gen. nov., sp. nov., isolated from wetland

Abstract

A novel orange-pigmented bacterial strain, designated HME6815 T , was isolated from wetland in Jeju Island, Republic of Korea. The cells were Gram stain-negative, non-motile, strictly aerobic and rod-shaped. Optimal growth occurred at 30 8C and pH 7.0 on R2A agar. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain HME6815 T formed a distinct phyletic lineage within the family Chitinophagaceae and was most closely related to members of the genera Sediminibacterium, Vibrionimonas, Hydrobacter, Hydrotalea and Asinibacterium with 92.3-94.3 % 16S rRNA gene sequence similarity. The major cellular fatty acids were iso-C 15 : 0 , iso-C 17 : 0 3-OH, summed feature 3 (C 16 : 1 v7c and/or C 16 : 1 v6c) and iso-C 13 : 0 . The only respiratory quinone was MK-7.Polar lipid analysis revealed the presence of phosphatidylethanolamine, four unidentified aminolipids, one unidentified aminophospholipid and three unidentified polar lipids. The DNA G+C content was 38.4 mol%. On the basis of the evidence presented in this study, strain HME6815 T represents a novel species of a new genus in the family Chitinophagaceae, for which the name Parasediminibacterium paludis gen. nov., sp. nov. is proposed. The type strain of the type species is HME6815 T (5KCTC 23736 T 5CECT 8010 T ).

Analysis of 1,000 Type-Strain Genomes Improves Taxonomic Classification of Bacteroidetes

“…When H. penzbergensis was proposed, V. magnilacihabitans was not considered; as shown in Table 3, H. penzbergensis is a later heterotypic synonym of V. magnilacihabitans . These two genera as well as Asinibacterium (Lee D.-G. et al, 2013) and even Hydrotalea (Kämpfer et al, 2011b; Albuquerque et al, 2012) and Parasediminibacterium (Kang et al, 2016) display phenotypic features similar to those of Sediminibacterium , the only known differences being presence or absence of motility, which may well be homoplastic given the findings reported above (Table 1), and a negative response for oxidase and catalase activities reported for Asinibacterium (Supplementary Table 1). Consequently, it is proposed that Asinibacterium lactis and Vibrionimonas magnilacihabitans be classified within Sediminibacterium .…”

Pseudobacter ginsenosidimutans gen. nov., sp. nov., isolated from ginseng cultivating soil

“…Similarly, some affiliates of this family show diphosphatidylglycerol (DPG) and phosphatidylethanolamine (PE) as the major polar lipids. At the time of writing, the family Chitinophagaceae contained 31 genera including the following recently described genera with validly published names: Hydrobacter (Eder et al, 2015), Parasediminibacterium (Kang et al, 2016), Flaviaesturariibacter (Kang et al, 2015), Arachidicoccus (Madhaiyan et al, 2015), Thermoflavifilum (Anders et al, 2014), Vibrionimonas (Albert et al, 2014) and Cnuella (Zhao et al, 2014). Based on 16S rRNA gene sequence similarity, the family Chitinophagaceae possesses not only a large number of genera but also shows wide phylogenetic extent between species (minimum 90 %) in the family (Hanada et al, 2014).…”

Microbial communities in the estuarine water areas of the rivers in the southeastern part of Lake Baikal

“…The identified bacteria of the genera Flavobacterium and Fluviicola are capable of decomposing plant polysaccharides, such as starch, cellulose, xylans and pectins, as well as photosynthesizing due to the presence of rhodopsins in the cells (Humphry et al, 2001;Martinez-Garcia et al, 2011;Feng et al, 2015;Park et al, 2017). Additionally, bacteria of the genera Arcicella and Parasediminibacterium play an important role in protein metabolism (Sheu et al, 2010;Kang et al, 2016), whereas the Paludibacter members ferment sugars (Ueki, 2006). Microbiome in the Solzan River had a wider range of bacterial taxa ( Fig.…”