Paraoxonase prevents accumulation of lipoperoxides in low‐density lipoprotein

Mackness, Michael I.; Arrol, Sharon; Durrington, Paul N.

doi:10.1016/0014-5793(91)80962-3

Cited by 897 publications

(613 citation statements)

References 15 publications

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“…PON is an antioxidant enzyme found in the liver, arterial wall, and plasma, where it travels attached to HDL (41). It has an important action in preventing LDL oxidation by peroxynitrite, in turn preventing the generation of oxidized LDL in plasma (12). Apo A-I stabilizes the enzyme (41), hence, interference with Apo A-I or with the HDL molecule itself could cause a reduction in the activity of the enzyme.…”

Section: Discussionmentioning

confidence: 99%

“…Paraoxonase (PON) is an enzyme with antioxidant activity, which circulates in plasma attached to HDL. Its function is to prevent oxidation of low-density lipoprotein (LDL), accounting thus for the antioxidant effect of HDL and explaining why HDL has a protective effect against atherosclerosis (12,13). PON activity has been shown to decrease with age (14) and increase with intake of lipid-lowering drugs (15).…”

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confidence: 99%

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Antibodies to high‐density lipoprotein and β₂‐glycoprotein I are inversely correlated with paraoxonase activity in systemic lupus erythematosus and primary antiphospholipid syndrome

Alves

Ames

Donohue

et al. 2002

Arthritis & Rheumatism

185

129

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Objective. To determine the prevalence of antihigh-density lipoprotein (anti-HDL) antibodies and to establish a possible relationship between anti-HDL, anticardiolipin antibodies (aCL), anti-␤ 2 -glycoprotein I (anti-␤ 2 GPI), and paraoxonase (PON) activity in patients with systemic lupus erythematosus (SLE) and primary antiphospholipid syndrome (APS).Methods. Thirty-two patients with SLE and 36 with primary APS were enrolled in a cross-sectional study. Twenty age-and sex-matched healthy subjects were used as controls. Serum levels of IgG and IgM aCL, anti-␤ 2 GPI, and antiprothrombin antibodies and IgG anti-HDL were measured by enzyme-linked immunosorbent assay. Total cholesterol, HDL cholesterol, HDL 2 , and HDL 3 were determined by standard enzymatic techniques. PON activity was assessed by quantification of nitrophenol formation, and total antioxidant capacity (TAC) by chemiluminescence.Results. Levels of total HDL, HDL 2 , and HDL 3 were reduced in patients with SLE compared with controls (mean ؎ SD 0.51 ؎ 0.3, 0.37 ؎ 0.3, and 0.14 ؎ 0.1 mmoles/liter, respectively, versus 1.42 ؎ 0.9, 1.01 ؎ 0.7, and 0.40 ؎ 0.2). Patients with SLE and primary APS had higher titers of anti-HDL antibodies and lower PON activity than controls. In the SLE population, PON activity was inversely correlated with IgG anti-HDL titers (r ‫؍‬ ؊0.48, P ‫؍‬ 0.005) whereas in the primary APS population, IgG anti-␤ 2 GPI was the only independent predictor of PON activity (r ‫؍‬ ؊0.483, P ‫؍‬ 0.003). In the SLE group, anti-HDL was inversely correlated with TAC (r ‫؍‬ ؊0.40, P < 0.02), and PON activity was positively correlated with TAC (r ‫؍‬ 0.43, P < 0.02).Conclusion. IgG anti-HDL and IgG anti-␤ 2 GPI antibodies are associated with reduced PON activity in patients with SLE and primary APS. Since the physiologic role of PON is to prevent low-density lipoprotein oxidation with its attendant atherogenic effects, the reported interactions may be relevant to the development of atherosclerosis in SLE and primary APS.

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

Antibodies to high‐density lipoprotein and β₂‐glycoprotein I are inversely correlated with paraoxonase activity in systemic lupus erythematosus and primary antiphospholipid syndrome

Alves

Ames

Donohue

et al. 2002

Arthritis & Rheumatism

185

129

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“…In the past few decades, it has become evident that high density lipoprotein (HDL) has antioxidant properties that protect LDL and HDL particles from oxidation. During this process, the HDLassociated enzyme paraoxonase1 (PON1) has been suggested as a possible mechanistic cause of this phenomenon [3,28,29,33,49]. PON1 belongs to the paraoxonase (PON) gene family which is located on chromosome 7q21.3-22.1 [37].…”

Section: Introductionmentioning

confidence: 99%

“…PON1 has protective action against atherosclerosis in both in vitro and in vivo models. Indeed, PON1 protects LDL particles against copper-induced lipid oxidation in vitro [3,28,29] and transgenic mice overexpressing the human PON1 gene have reduced atherosclerotic lesions [48]. Moreover, PON1 knockout mice display accelerated atherosclerosis progression and increased lipid oxidation [40,42,43].…”

Section: Introductionmentioning

confidence: 99%

Effect of atorvastatin on paraoxonase1 (PON1) and oxidative status

Nagila

Permpongpaiboon

Tantrarongroj

et al. 2009

Pharmacological Reports

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Abstract:It has been proposed that paraoxonase1 (PON1), a high density lipoprotein (HDL)-associated esterase/lactonase, has antiatherosclerotic properties. The activity of PON1 is influenced by PON1 polymorphisms. However, the influence of PON1 polymorphisms on PON1 activity and oxidative stress in response to lipid-lowering drugs remains poorly understood. The objective of the present study was to investigate the effects of atorvastatin on PON1 activity and oxidative status. The influence of PON1 polymorphisms on PON1 activity and oxidative status in response to atorvastatin treatment was also evaluated. In total, 22 hypercholesterolemic patients were treated with atorvastatin at a dose of 10 mg/day for 3 months. Lipid profile, lipid oxidation markers (malondialdehyde (MDA), conjugated diene (CD), total peroxides (TP)), total antioxidant substance (TAS), oxidative stress index (OSI), and paraoxonase1 activity were determined before and after treatment. L55M, Q192R, and T(-107)C PON1 polymorphisms were also determined. Atorvastatin treatment significantly reduced the levels of total cholesterol (24.5%), low density lipoprotein (LDL) cholesterol (25.4%), triglycerides (24.4%), CD (4.4%), MDA (15.2%), TP (13.0%) and OSI (24.0%), and significantly increased the levels of TAS (27.3%), and PON1 activity (14.0%). Interestingly, the increase in PON1 activity and the reduction in oxidative stress in response to atorvastatin were influenced only by the PON1 T-107C polymorphism. Atorvastatin treatment improved the lipid profile, lipid oxidation, and oxidative/antioxidative status markers including the activity of PON1 towards paraoxon. These beneficial effects may be attributed to the antioxidant properties of statins and the increase in PON1 activity. The increase in PON1 activity was enhanced by the PON1 T-107C polymorphism.

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“…The oxidation of NADPH was monitored at 340 nm and the activity was expressed as nmol oxidized NADPH/min/mg protein. The paraoxonase (PON1) activity was determined based from the method described by Mackness et al (1991). Briefly, 50 µl of serum was added to 1 ml Tris/HCl buffer (100 mM, pH 8.0) containing 2 mM CaCl 2 and 5.5 mM paraoxon.…”

Section: Determination Of Hepatic Lipid-regulating and Antioxidant Enmentioning

confidence: 99%

Enhancement of Lipid Metabolism and Antioxidant Defense Status in Mice Fed with High Fat Diet Supplemented with Antheraea pernyi Silk Fibroin Powder

Um¹,

Rico²,

Kweon³

et al. 2011

International Journal of Industrial Entomology

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The effect of diet supplementation of Antheraea pernyi (A. pernyi) silk fibroin on the lipid metabolism and antioxidant defense status in high fat-fed mice was investigated. The animals were given normal control diet (NC group), high fat diet (HF group), or high fat diet supplemented with A. pernyi silk fibroin powder (HFS group) for 7 weeks. After the experimental period, the HF group showed significant increase in body weight, plasma and hepatic total cholesterol levels, and hepatic triglyceride concentration, and decreased activities of hepatic antioxidant enzymes relative to NC group. However, the HFS group exhibited marked reduction in body weight, plasma cholesterol and hepatic triglyceride levels, hepatic lipogenic enzyme activities, and lipid peroxidation rate, and higher high-density lipoprotein (HDL)-cholesterol level, fecal triglyceride content, and antioxidant enzymes activities compared with that of HF group. These findings demonstrate that dietary feeding of A. pernyi silk fibroin could improve the lipid metabolism and antioxidant defense system via regulation of hepatic antioxidant and lipogenic enzymes activities. Hence, this silk fibroin may be beneficial as a functional biomaterial for the development of therapeutic agent against high fat diet-induced hyperlipidemia and its related diseases.

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Paraoxonase prevents accumulation of lipoperoxides in low‐density lipoprotein

Cited by 897 publications

References 15 publications

Antibodies to high‐density lipoprotein and β₂‐glycoprotein I are inversely correlated with paraoxonase activity in systemic lupus erythematosus and primary antiphospholipid syndrome

Antibodies to high‐density lipoprotein and β₂‐glycoprotein I are inversely correlated with paraoxonase activity in systemic lupus erythematosus and primary antiphospholipid syndrome

Effect of atorvastatin on paraoxonase1 (PON1) and oxidative status

Enhancement of Lipid Metabolism and Antioxidant Defense Status in Mice Fed with High Fat Diet Supplemented with Antheraea pernyi Silk Fibroin Powder

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Paraoxonase prevents accumulation of lipoperoxides in low‐density lipoprotein

Cited by 897 publications

References 15 publications

Antibodies to high‐density lipoprotein and β2‐glycoprotein I are inversely correlated with paraoxonase activity in systemic lupus erythematosus and primary antiphospholipid syndrome

Antibodies to high‐density lipoprotein and β2‐glycoprotein I are inversely correlated with paraoxonase activity in systemic lupus erythematosus and primary antiphospholipid syndrome

Effect of atorvastatin on paraoxonase1 (PON1) and oxidative status

Enhancement of Lipid Metabolism and Antioxidant Defense Status in Mice Fed with High Fat Diet Supplemented with Antheraea pernyi Silk Fibroin Powder

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Product

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Antibodies to high‐density lipoprotein and β₂‐glycoprotein I are inversely correlated with paraoxonase activity in systemic lupus erythematosus and primary antiphospholipid syndrome

Antibodies to high‐density lipoprotein and β₂‐glycoprotein I are inversely correlated with paraoxonase activity in systemic lupus erythematosus and primary antiphospholipid syndrome