Paramyxovirus RNA Synthesis and P Gene Expression

Kolakofsky, Daniel; Vidal, S; Curran, Joseph

doi:10.1007/978-1-4615-3790-8_7

Cited by 23 publications

(20 citation statements)

References 59 publications

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“…The paramyxovirus genome is used with great efficiency, with over 95 % of the RNA encoding the viral proteins. An apparently universal feature of paramyxoviruses is the use of alternative reading frames and RNA editing within their P genes resulting in increased coding capacity of the genome (for review, see Kolakofsky et al, 1990). The translation products of mRNAs transcribed from alternative open reading frames (ORFs) within the P gene appear to be non-structural viral proteins present in the infected cell but absent from the virus particle.…”

Section: Introductionmentioning

confidence: 99%

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RNA editing in Newcastle disease virus

Steward

Vipond

Millar

et al. 1993

Journal of General Virology

288

201

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The co-transcriptional editing of the Newcastle disease virus (NDV) P gene has been studied by sequence analysis of cloned viral genomic RNA and mRNA. Evidence has been obtained for the specific insertion of non-templated G nucleotides, the consequence of which is the generation of three populations of P gene-derived mRNAs. The three populations encode proteins (P, V and W) which have a common N-terminal region, but which utilize three different reading frames at their C termini. Paradoxically, NDV edits its P gene mRNA by the insertion of non-templated G residues in a manner similar to Sendai and measles viruses (P-~ V editing) despite its apparent closer evolutionary relationship to the simian virus type 5, mumps and related group of viruses which edit a V genomic sequence to generate an mRNA to encode a functional P protein (V -~ P editing).

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Section: Introductionmentioning

confidence: 99%

“…Non-templated G residues are inserted at a specific site within the P gene, the consequence of which is the utilization of alternative reading frames during translation (Kolakofsky et aL, 1990). Furthermore, the editing has been found to occur in two distinct patterns.…”

Section: Introductionmentioning

confidence: 99%

RNA editing in Newcastle disease virus

Steward

Vipond

Millar

et al. 1993

Journal of General Virology

288

201

View full text Add to dashboard Cite

show abstract

“…A similar phenomenon occurred in SV5, although in this instance, editing by the addition of two extra G residues was required to generate the P protein, the V protein being encoded by an mRNA faithfully transcribed from the genome. The insertion is thought to occur as a result of the viral polymerase "stuttering" on a particular sequence within the template [6,20,21]. The 36 kDa…”

mentioning

confidence: 99%

The Newcastle disease virus V protein binds zinc

Steward

Samson

Errington

et al. 1995

Archives of Virology

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The V protein of Newcastle disease virus (NDV) is produced by the insertion of a single nontemplated G residue at a specific point during transcription of the phosphoprotein (P) gene, accessing a new reading frame upon translation. The V protein, in common with its counterpart in other paramyxoviruses contains a highly cysteine rich motif near the carboxyl terminus, suggestive of a zinc-binding domain. By constructing E. coli overexpression plasmids for the NDV P and V proteins, and monitoring the binding of 65ZnCl2 to proteins electroblotted onto nitrocellulose membranes, we have demonstrated that the V protein strongly binds zinc.

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“…Some viral mRNAs encode multiple proteins in distinct reading frames. Among the most striking examples is the PIC gene of paramyxoviruses, which encodes as many as nine distinct polypeptide products in overlapping reading frames that are accessed by alternative initiation codons as well as by RNA editing (Kolakofsky et al, 1991).…”

Section: Protein Synthesis and Modificationmentioning

confidence: 99%