Parameters Affecting the Performance of Immobilized Enzyme

Abstract

Enzyme immobilization has been investigated to improve lipase properties over the past few decades. Different methods and various carriers have been employed to immobilize enzyme. However, the application of enzymatic technology in large scale is rarely seen during the industrial process. The main obstacles are a high cost of the immobilization and the poor performance of immobilized lipase. This review focuses on the current status of enzyme immobilization, which aims to summarize the latest research on the parameters affecting the performance of immobilized enzyme. Particularly, the effect of immobilization methods, immobilization carriers, and enzyme loading has been discussed.

Development of a Magnetic Microbead Affinity Selection Screen (MagMASS) Using Mass Spectrometry for Ligands to the Retinoid X Receptor-α

“…Furthermore, immobilization through random lysine and arginine residues, especially through multipoint attachment to the support, can change the functionality of the protein. Although immobilization of receptors and enzymes in some orientations can block access of ligands to the active site [20], selective protein immobilization can retain activity while enhancing stability such as resistance to denaturation [21, 22]. …”

Enzyme Immobilization for Organic Synthesis

“…Some other important parameters such as surface area and pore size can be modulated [36,37]. The sol-gel process involves hydrolyzing silane precursors (tetra-alkoxysilanes and alkoxysilane) followed by their polycondensation to form ceramic materials [38]. Conventional sol-gel matrices have tight pores of approximately 1.5 nm diameter [28,39].…”

High expression and biosilica encapsulation of alkaline-active carbonic anhydrase for CO2 sequestration system development

“…4A, the effect of TMOS amount on immobilization efficiency and residual activity of HCA(SP-)@silica was investigated. An excess amount of TMOS can reduce the residual activity of encapsulated HCA(SP-), due to either steric hindrance of arbitrarily formed silica on the CA active site or inward mass transfer of the substrate (Zhang et al, 2013). However, the use of a low amount of TMOS led to leaching of the encapsulated HCA(SP-).…”